Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	916,848	A→G	T126A (ACA→GCA)	ybjD →	DUF2813 domain‑containing protein YbjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	916,848	0	A	G	100.0%	151.3 / NA	42	T126A (ACA→GCA)	ybjD	DUF2813 domain‑containing protein YbjD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/20);  total (22/20)

CCGCGAATCGCTGCCAGGCCGACATCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAA  >  NC_000913/916712‑916981                                                                                                                                         |                                                                                                                                      ccGCGAATCGCTGCCAGGCCGACATCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGgcac                                                                                                                                    >  2:655552/1‑140 (MQ=255) ccGCGAATCGCTGCCAGGCCGACATCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGgcac                                                                                                                                    >  1:121073/1‑140 (MQ=255)   gcgAATCGCTGCCAGGCCGACATCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTg                                                                                                                                  <  2:892865/140‑1 (MQ=255)           cTGCCAGGCCGACATCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGttt                                                                                                                          >  1:599177/1‑140 (MQ=255)                          cGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACgg                                                                                                           <  1:1068960/140‑1 (MQ=255)                           gggTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGa                                                                                                          <  2:599177/140‑1 (MQ=255)                             gTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACa                                                                                                        <  1:655552/140‑1 (MQ=255)                                     ttATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTg                                                                                                <  2:971770/140‑1 (MQ=255)                                       aTCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGAt                                                                                              >  1:86950/1‑140 (MQ=255)                                       aTCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGAt                                                                                              >  1:71428/1‑140 (MQ=255)                                             cGCTGGAAGCGTGCTGGACGCCATGCACCGATGACTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAg                                                                                        <  2:968386/140‑1 (MQ=255)                                                  gAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGatat                                                                                   <  1:983647/140‑1 (MQ=255)                                                  gAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCACAGTTTTCTCGATAAAGATGGACATCCGATTGATGTCGAGGatat                                                                                   <  2:644508/140‑1 (MQ=255)                                                          cTGGACGCCATGCACCGATGGATATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGTCGGACATCCGATTGATGTCGAGGATATTAACGATc                                                                           >  2:817716/1‑140 (MQ=255)                                                             gACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAgg                                                                        >  1:255625/1‑140 (MQ=255)                                                               cGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCa                                                                      >  2:676375/1‑140 (MQ=255)                                                                  cATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCGGGCACGc                                                                   >  2:631365/1‑140 (MQ=255)                                                                         cGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTgg                                                            >  1:664703/1‑140 (MQ=255)                                                                           aTGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGAt                                                                            >  1:689998/1‑122 (MQ=255)                                                                           aTGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGAt                                                                            <  2:689998/122‑1 (MQ=255)                                                                           aTGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTg                                                          >  1:691756/1‑140 (MQ=255)                                                                               cTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGtt                                                      <  2:255625/140‑1 (MQ=255)                                                                                      cGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGcc                                               >  1:185135/1‑140 (MQ=255)                                                                                          ttttttATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGtg                                           <  1:310130/140‑1 (MQ=255)                                                                                                  cGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCtt                                   <  2:260854/140‑1 (MQ=255)                                                                                                  cGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCtt                                   >  1:260854/1‑140 (MQ=255)                                                                                                    tCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGtg                                           >  1:114197/1‑130 (MQ=255)                                                                                                    tCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGtg                                           <  2:114197/130‑1 (MQ=255)                                                                                                           ggggAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGc                          <  2:185135/140‑1 (MQ=255)                                                                                                             ggAGAGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGccc                        >  1:1137471/1‑140 (MQ=255)                                                                                                              gagaGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCg                       >  2:617475/1‑140 (MQ=255)                                                                                                              gagaGTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCg                       >  2:944374/1‑140 (MQ=255)                                                                                                               agagTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGt                      >  1:7677/1‑140 (MQ=255)                                                                                                                  gTGCGGAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGtttt                   >  1:564032/1‑140 (MQ=255)                                                                                                                       gAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCGGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATgcg              <  1:631365/140‑1 (MQ=255)                                                                                                                       gAAGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATgcg              <  1:944374/140‑1 (MQ=255)                                                                                                                         aGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGcc            <  2:691756/140‑1 (MQ=255)                                                                                                                         aGACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACACCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGcc            <  1:359419/140‑1 (MQ=255)                                                                                                                          gACGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCg           <  2:7677/140‑1 (MQ=255)                                                                                                                           aCGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATg                >  1:1018680/1‑134 (MQ=255)                                                                                                                           aCGGCAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATCGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATg                <  2:1018680/134‑1 (MQ=255)                                                                                                                               cAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTc      >  1:70718/1‑140 (MQ=255)                                                                                                                               cAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTc      >  1:618806/1‑140 (MQ=255)                                                                                                                               cAGCGTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTc      >  1:323113/1‑140 (MQ=255)                                                                                                                                   gTGATGGCACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTaa  >  1:45584/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CCGCGAATCGCTGCCAGGCCGACATCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAA  >  NC_000913/916712‑916981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑