Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	917,634	A→G	T388A (ACG→GCG)	ybjD →	DUF2813 domain‑containing protein YbjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	917,634	0	A	G	100.0%	190.6 / NA	52	T388A (ACG→GCG)	ybjD	DUF2813 domain‑containing protein YbjD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

AGTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAACGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGATTGA  >  NC_000913/917497‑917767                                                                                                                                          |                                                                                                                                      ggTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGggaagcg                                                                                                                                     <  2:868458/139‑1 (MQ=255)  gTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGggaagcgg                                                                                                                                    >  1:1134167/1‑140 (MQ=255)  gTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGggaagcg                                                                                                                                     >  2:832134/1‑139 (MQ=255)     cTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGggaagcggaaa                                                                                                                                 >  1:1051536/1‑140 (MQ=255)     cTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGggaagcggaaa                                                                                                                                 >  2:1064181/1‑140 (MQ=255)     cTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGggaagcggaaa                                                                                                                                 >  2:338586/1‑140 (MQ=255)       cgcgcgTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAAcc                                                                                                                               <  1:1064181/140‑1 (MQ=255)        gcgcgTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCt                                                                                                                              <  1:865774/140‑1 (MQ=255)        gcgcgTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCt                                                                                                                              >  2:916168/1‑140 (MQ=255)        gcgcgTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCAGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCt                                                                                                                              >  2:438553/1‑140 (MQ=255)         cgcgTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTg                                                                                                                             >  1:102937/1‑140 (MQ=255)            gTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGt                                                                                                                          <  2:736893/140‑1 (MQ=255)                        cGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGt                                                                                                                          >  1:382792/1‑128 (MQ=255)                        cGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGt                                                                                                                          <  2:382792/128‑1 (MQ=255)                        cGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACt                                                                                                              >  1:1036224/1‑140 (MQ=255)                              gggCCGAGTGGCTTGAGTACCGAAGATAGCAGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGcgcg                                                                                                        <  1:438553/140‑1 (MQ=255)                                  cGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAg                                                                                                    <  2:1051536/140‑1 (MQ=255)                                    aGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTg                                                                                                  >  1:244255/1‑140 (MQ=255)                                     gTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCCGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGc                                                                                                 <  2:1009635/140‑1 (MQ=255)                                        gCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGa                                                                                              <  1:771857/140‑1 (MQ=255)                                         cTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGAc                                                                                             <  1:687340/140‑1 (MQ=255)                                             aGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTAGCAATGAACTGGCGCGTCAGTGCGGAcatca                                                                                         >  2:711696/1‑140 (MQ=255)                                                  cGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCg                                                                                                >  1:159720/1‑128 (MQ=255)                                                  cGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCg                                                                                                <  2:159720/128‑1 (MQ=255)                                                  cGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTg                                                                                    >  2:970708/1‑140 (MQ=255)                                                     aGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATg                                                                                 <  2:102937/140‑1 (MQ=255)                                                         aGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGa                                                                             >  1:1061250/1‑140 (MQ=255)                                                         aGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGa                                                                             <  2:1134167/140‑1 (MQ=255)                                                          gCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGaa                                                                            <  2:244255/140‑1 (MQ=255)                                                           ccGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAg                                                                           <  1:970708/140‑1 (MQ=255)                                                                gCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATc                                                                      <  2:716961/140‑1 (MQ=255)                                                                   tatCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAg                                                                   >  2:913338/1‑140 (MQ=255)                                                                        tttCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCAt                                                              <  2:1061250/140‑1 (MQ=255)                                                                           cacaTTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGa                                                           >  2:33024/1‑140 (MQ=255)                                                                            acaTTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAg                                                          >  2:866168/1‑140 (MQ=255)                                                                             caTTCGTTTTAACCGTCCGTCATCGCTGTTCGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGt                                                         <  1:913338/140‑1 (MQ=255)                                                                                tCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTg                                                      >  1:759827/1‑140 (MQ=255)                                                                                  gTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGcc                                                    <  2:759827/140‑1 (MQ=255)                                                                                  gTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGcc                                                    <  1:916168/140‑1 (MQ=255)                                                                                        aCCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTcc                                              <  1:866168/140‑1 (MQ=255)                                                                                            tccgtcATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGc                                          >  1:206709/1‑140 (MQ=255)                                                                                            tccgtcATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGc                                          >  2:552536/1‑140 (MQ=255)                                                                                              cgtcATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAA‑GTCATTGAGTTTGCCCAGTCCGGGCTaa                                       >  2:356782/1‑140 (MQ=255)                                                                                                       tGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTg                               >  1:970686/1‑140 (MQ=255)                                                                                                         tttGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCa                                  <  2:1093441/135‑1 (MQ=255)                                                                                                         tttGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCa                                  >  1:1093441/1‑135 (MQ=255)                                                                                                           tGCACGCTGCTGGTTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTa                           >  2:268201/1‑140 (MQ=255)                                                                                                           tGCACGCTGCTGATTGCTGGTGGAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTa                           >  2:203512/1‑140 (MQ=255)                                                                                                                            tggtggAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATg          >  1:685533/1‑140 (MQ=255)                                                                                                                            tggtggAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATg          >  2:890410/1‑140 (MQ=255)                                                                                                                            tggtggAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATg          >  1:167157/1‑140 (MQ=255)                                                                                                                            tggtggAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATg          >  2:1113826/1‑140 (MQ=255)                                                                                                                              gtggAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATggg        >  1:987313/1‑140 (MQ=255)                                                                                                                                 gAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGAt     >  2:6017/1‑140 (MQ=255)                                                                                                                                 gAAGGGGAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGAt     <  2:987313/140‑1 (MQ=255)                                                                                                                                    ggggAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGATTGa  <  1:6017/140‑1 (MQ=255)                                                                                                                                    ggggAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGATTGa  <  1:203512/140‑1 (MQ=255)                                                                                                                                    ggggAAGCGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGATTGa  <  2:970686/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AGTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGGCTTGAGTACCGAAGATAGCCGACGCATATCCTTTCACATTCGTTTTAACCGTCCGTCATCGCTGTTTGCACGCTGCTGGTTGCTGGTGGAAGGGGAAACGGAAACCTGGGTTATCAATGAACTGGCGCGTCAGTGCGGACATCATTTTGATGCCGAAGGGATCAAGGTCATTGAGTTTGCCCAGTCCGGGCTAAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGATTGA  >  NC_000913/917497‑917767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑