Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	946,407	T→C	R179R (CGT→CGC)	ycaD →	putative transporter YcaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	946,407	0	T	C	100.0%	257.4 / NA	70	R179R (CGT→CGC)	ycaD	putative transporter YcaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/32);  total (38/32)

CGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGCCTGATG  >  NC_000913/946271‑946539                                                                                                                                         |                                                                                                                                     cGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGcgtg                                                                                                                                   >  1:511645/1‑140 (MQ=255)           ggTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGcgtg                                                                                                                                   <  1:56556/130‑1 (MQ=255)           ggTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGcgtg                                                                                                                                   >  2:56556/1‑130 (MQ=255)             tttATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCgcgt                                                                                                                                    >  1:898648/1‑127 (MQ=255)             tttATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATcagcag                                                                                                                       >  2:751057/1‑140 (MQ=255)             tttATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATcagcag                                                                                                                       >  1:940795/1‑140 (MQ=255)             tttATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATcagcag                                                                                                                       >  2:521320/1‑140 (MQ=255)             tttATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATcagcag                                                                                                                       >  1:544770/1‑140 (MQ=255)               tattaCGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGc                                                                                                                     >  2:195458/1‑140 (MQ=255)                attaCGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCt                                                                                                                    >  2:261521/1‑140 (MQ=255)                attaCGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCt                                                                                                                    <  1:458311/140‑1 (MQ=255)                   aCGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGaa                                                                                                                 <  2:811595/140‑1 (MQ=255)                      tgagAACGTTCTTAGTCCAGGTACTGAGCAGCAGGCGCTCAACCGGGCTGATGTCGAAATTGCCGCGGGTTACAGGTTTGACGCTGGCAGGGATCTTACCGCTGATGTTTATGCGCGTGCGGAATCAGCAGGCTGAAAAc                                                                                                              >  2:689269/4‑140 (MQ=255)                       gggAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAAcc                                                                                                             >  2:731029/1‑140 (MQ=255)                         gAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCAt                                                                                                           >  1:18474/1‑140 (MQ=255)                         gAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCAt                                                                                                           <  2:511645/140‑1 (MQ=255)                             gTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGAtt                                                                                                       <  2:25245/140‑1 (MQ=255)                              tttttAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTc                                                                                                      >  1:497565/1‑140 (MQ=255)                                tttAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGa                                                                                                    <  1:521320/140‑1 (MQ=255)                                tttAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGa                                                                                                    <  1:731029/140‑1 (MQ=255)                                tttAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGa                                                                                                    >  1:92888/1‑140 (MQ=255)                                 ttAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGAc                                                                                                   <  2:563458/140‑1 (MQ=255)                                        aGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAAtt                                                                                            >  2:955200/1‑140 (MQ=255)                                         gTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTa                                                                                           >  1:622259/1‑140 (MQ=255)                                             cTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTc                                                                                       <  2:497565/140‑1 (MQ=255)                                              tGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCa                                                                                      >  1:239060/1‑140 (MQ=255)                                                   aGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCaa                                                                                     <  1:577692/136‑1 (MQ=255)                                                   aGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCaa                                                                                     >  2:577692/1‑136 (MQ=255)                                                   aGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCt                                                                                 >  1:445096/1‑140 (MQ=255)                                                   aGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCt                                                                                 >  1:332627/1‑140 (MQ=255)                                                    gCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTa                                                                                >  2:1100655/1‑140 (MQ=255)                                                    gCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTa                                                                                >  1:1133992/1‑140 (MQ=255)                                                        aGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAAc                                                                            <  1:1013200/140‑1 (MQ=255)                                                             cAACCGAGCTGATGTCCATATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGt                                                                       <  2:244296/140‑1 (MQ=255)                                                                  gAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGgc                                                                  <  2:92888/140‑1 (MQ=255)                                                                    gCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGgcgc                                                                >  2:655348/1‑140 (MQ=255)                                                                    gCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGgcgc                                                                >  2:1096620/1‑140 (MQ=255)                                                                       gATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGc                                                             <  1:1096620/140‑1 (MQ=255)                                                                       gATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGc                                                             <  2:622259/140‑1 (MQ=255)                                                                       gATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGc                                                             <  2:332627/140‑1 (MQ=255)                                                                             cGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCg                                                       >  2:850763/1‑140 (MQ=255)                                                                               tATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTg                                                     <  1:195458/140‑1 (MQ=255)                                                                                     cGTGGGCTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGc                                               >  1:721728/1‑140 (MQ=255)                                                                                         ggTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCa                                           >  1:910623/1‑140 (MQ=255)                                                                                         ggTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCa                                           >  2:453810/1‑140 (MQ=255)                                                                                         ggTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCa                                           >  1:684312/1‑140 (MQ=255)                                                                                           ttACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCAtt                                         <  1:618345/140‑1 (MQ=255)                                                                                           ttACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCAtt                                         <  1:655348/140‑1 (MQ=255)                                                                                            tACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTa                                        >  1:249239/1‑140 (MQ=255)                                                                                                   ttGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAgg                                 >  2:783360/1‑140 (MQ=255)                                                                                                    tGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGa                                >  2:48641/1‑140 (MQ=255)                                                                                                     gACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGaa                               <  2:544770/140‑1 (MQ=255)                                                                                                     gACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGaa                               <  2:905099/140‑1 (MQ=255)                                                                                                         ttGGCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGt                           >  1:952489/1‑140 (MQ=255)                                                                                                           ggCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTc                         >  2:1116390/1‑140 (MQ=255)                                                                                                            gCAGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCt                        <  2:445096/140‑1 (MQ=255)                                                                                                             cAGGGATCTTACCGCTGTTGTTTACGCGCGTGTTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGaa                               >  2:236505/1‑132 (MQ=255)                                                                                                             cAGGGATCTTACCGCTGTTGTTTACGCGCGTGTTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGaa                               <  1:236505/132‑1 (MQ=255)                                                                                                              aGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTgg                      <  2:940795/140‑1 (MQ=255)                                                                                                              aGGGATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTgg                      <  2:684312/140‑1 (MQ=255)                                                                                                               gggATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTggg                     <  2:721728/140‑1 (MQ=255)                                                                                                                 gATCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGAt                   <  2:1133992/140‑1 (MQ=255)                                                                                                                  aTCTTACCGCTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGAtc                  <  1:850763/140‑1 (MQ=255)                                                                                                                           cTGTTGTTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATgg         >  1:322251/1‑140 (MQ=255)                                                                                                                            tgttgtTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGc        >  2:685541/1‑140 (MQ=255)                                                                                                                            tgttgtTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGc        >  2:1046249/1‑140 (MQ=255)                                                                                                                            tgttgtTTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGc        >  1:79353/1‑140 (MQ=255)                                                                                                                             gttgttTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGcc       <  1:48641/140‑1 (MQ=255)                                                                                                                             gttgttTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCATGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGcc       <  1:261521/140‑1 (MQ=255)                                                                                                                              ttgttTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTgg                      <  1:288491/124‑1 (MQ=255)                                                                                                                              ttgttTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTgg                      >  2:288491/1‑124 (MQ=255)                                                                                                                              ttgttTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGCCt      >  2:742232/1‑140 (MQ=255)                                                                                                                              ttgttTACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGCCt      >  1:666613/1‑140 (MQ=255)                                                                                                                                 tttACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGCCTGAt   <  2:322251/140‑1 (MQ=255)                                                                                                                                  ttACGCGCGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGCCTGATg  <  2:79353/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCCGTCAGGCGCGGCTTGGCGTGAATGGCTGCATTATCTCAGGAATCGTTCTGGGATCTCTATATGGCCTGATG  >  NC_000913/946271‑946539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑