Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	985,303	A→G	V136A (GTC→GCC)	aspC ←	aspartate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	985,303	0	A	G	100.0%	178.2 / NA	49	V136A (GTC→GCC)	aspC	aspartate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/22);  total (27/22)

GCAGCAGCCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAGCACGTTTG  >  NC_000913/985167‑985434                                                                                                                                         |                                                                                                                                    gcagcaGCCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGgcgc                                                                                                                                  <  1:75713/140‑1 (MQ=255)     cagcCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCTGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCtt                                                                                                                              >  1:1019566/1‑140 (MQ=255)     cagcCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCtt                                                                                                                              >  2:60773/1‑140 (MQ=255)           tGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGtt                                                                                                                        >  1:564792/1‑140 (MQ=255)              aaCAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCgg                                                                                                                     <  2:1109774/140‑1 (MQ=255)              aaCAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCgg                                                                                                                     <  1:60773/140‑1 (MQ=255)              aaCAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCgg                                                                                                                     <  2:331618/140‑1 (MQ=255)                  gCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAg                                                                                                                 >  1:118315/1‑140 (MQ=255)                  gCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAg                                                                                                                 >  2:206777/1‑140 (MQ=255)                        cGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCgg                                                                                                                     >  2:297235/1‑130 (MQ=255)                        cGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCgg                                                                                                                     <  1:297235/130‑1 (MQ=255)                        cGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTggg                                                                                                           >  1:357995/1‑140 (MQ=255)                             ccAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCt                                                                                                      >  2:440751/1‑140 (MQ=255)                              cAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTc                                                                                                     <  1:512486/140‑1 (MQ=255)                                gCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCAc                                                                                                   <  2:564792/140‑1 (MQ=255)                                    gAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCcac                                                                                               <  1:440751/140‑1 (MQ=255)                                     aGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCTGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCcaca                                                                                              <  2:1019566/140‑1 (MQ=255)                                     aGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCcac                                                                                               <  2:164637/139‑1 (MQ=255)                                     aGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCcac                                                                                               >  1:164637/1‑139 (MQ=255)                                          cattcaGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCcacac                                                                                             >  1:997345/1‑136 (MQ=255)                                          cattcaGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCcacac                                                                                             <  2:997345/136‑1 (MQ=255)                                                             tGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTg                                                                      >  2:700612/1‑140 (MQ=255)                                                               cATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGcc                                                                    >  1:855840/1‑140 (MQ=255)                                                               cATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGcc                                                                    >  1:464886/1‑140 (MQ=255)                                                               cATCGAAGTCAAGAGTGTGATCTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGcc                                                                    >  2:465492/1‑140 (MQ=255)                                                                  cGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAgg                                                                 >  1:9715/1‑140 (MQ=255)                                                                         aaGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCgg                                                          <  2:9715/140‑1 (MQ=255)                                                                              tgtgATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGcc                                                     <  2:357995/140‑1 (MQ=255)                                                                              tgtgATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGcc                                                     >  2:39378/1‑140 (MQ=255)                                                                              tgtgATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGcc                                                     >  2:112676/1‑140 (MQ=255)                                                                                tgATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCAc                                                   >  2:1053031/1‑140 (MQ=255)                                                                                 gATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCAcg                                                  <  2:855840/140‑1 (MQ=255)                                                                                                     gCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGccccc                              >  2:497255/1‑140 (MQ=255)                                                                                                      cGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGcccccc                             <  2:557308/140‑1 (MQ=255)                                                                                                       gTATTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCg                            <  1:497255/140‑1 (MQ=255)                                                                                                         aTTCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGa                          >  2:243812/1‑140 (MQ=255)                                                                                                          ttCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCTCCCCGGAg                         >  2:955320/1‑140 (MQ=255)                                                                                                          ttCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAg                         >  2:539711/1‑140 (MQ=255)                                                                                                          ttCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAg                         <  1:206777/140‑1 (MQ=255)                                                                                                           tCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCg                            <  2:639991/136‑1 (MQ=255)                                                                                                           tCACGAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCg                            >  1:639991/1‑136 (MQ=255)                                                                                                               gAACTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCtgt                    >  1:290699/1‑140 (MQ=255)                                                                                                                  cTTCCAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGcc                 <  2:290699/140‑1 (MQ=255)                                                                                                                     ccAGACCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTg              >  1:382218/1‑140 (MQ=255)                                                                                                                         aCCTGCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAg          <  2:1034304/140‑1 (MQ=255)                                                                                                                             gCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAGCa        >  1:81651/1‑138 (MQ=255)                                                                                                                             gCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAGCa        <  2:81651/138‑1 (MQ=255)                                                                                                                             gCAGAGTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAGCACg      >  2:745057/1‑140 (MQ=255)                                                                                                                               agagTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAGCACGtt    <  2:643171/140‑1 (MQ=255)                                                                                                                                 agTTAAAGGCGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAGCACGTTTg  <  2:464886/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GCAGCAGCCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGCTCTTATGGTTCGGCCAGCTTGGGTTGCTCACCCACACACGCTTAACGCTGGTATTTTTTGCCAGGAAATCGGCAGCCACGCGTAGTGCGCCAGTGCCCCCCGGAGTCTGTGCCGTGCGAGCACGTTTG  >  NC_000913/985167‑985434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑