Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,023,429	A→G	V152A (GTC→GCC)	yccS ←	putative transporter YccS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,023,429	0	A	G	100.0%	218.5 / NA	60	V152A (GTC→GCC)	yccS	putative transporter YccS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/34);  total (26/34)

TGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGA  >  NC_000913/1023300‑1023563                                                                                                                                  |                                                                                                                                       tGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCa                                                                                                                              >  1:862549/1‑140 (MQ=255)   aTCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGa                                                                                                                            >  1:1109886/1‑140 (MQ=255)             tCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGc                                                                                                                  >  1:1000356/1‑140 (MQ=255)              cAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGcc                                                                                                                 >  2:1103122/1‑140 (MQ=255)                ggATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCcag                                                                                                               >  2:870409/1‑140 (MQ=255)                     aaaCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGAt                                                                                                          >  2:868807/1‑140 (MQ=255)                       aCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATACGTGTCAGGGCGTTGTACCAGACGGCACCgg                                                                                                                   >  1:474468/1‑129 (MQ=255)                       aCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCgg                                                                                                                   <  2:474468/129‑1 (MQ=255)                       aCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATAc                                                                                                        >  1:236483/1‑140 (MQ=255)                           gcgcGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTGAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCg                                                                                                    >  2:1028305/1‑140 (MQ=255)                           gcgcGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCg                                                                                                    >  1:766793/1‑140 (MQ=255)                           gcgcGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCg                                                                                                    >  1:977285/1‑140 (MQ=255)                           gcgcGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCg                                                                                                    >  1:293432/1‑140 (MQ=255)                            cgcgACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCgg                                                                                                   <  1:870409/140‑1 (MQ=255)                             gcgACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCgg                                                                                                   >  2:528166/1‑139 (MQ=255)                             gcgACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCgg                                                                                                   <  1:528166/139‑1 (MQ=255)                              cgACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGgct                                                                                                 <  2:647556/140‑1 (MQ=255)                                 cTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGgctgct                                                                                              >  1:986027/1‑140 (MQ=255)                                      gCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATAc                                                                                         <  1:1079198/140‑1 (MQ=255)                                       cTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATAcc                                                                                        <  1:257515/140‑1 (MQ=255)                                        tCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCa                                                                                       <  2:299501/140‑1 (MQ=255)                                         cAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAg                                                                                      <  2:1109886/140‑1 (MQ=255)                                         cAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAg                                                                                      <  2:766793/140‑1 (MQ=255)                                         cAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAg                                                                                      <  1:1028305/140‑1 (MQ=255)                                         cAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAg                                                                                      <  1:1103122/140‑1 (MQ=255)                                           aGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTg                                                                                    >  1:956926/1‑140 (MQ=255)                                            gATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGc                                                                                   <  2:573727/140‑1 (MQ=255)                                            gATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGc                                                                                   <  2:447282/140‑1 (MQ=255)                                                 cgcgCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATa                                                                              >  1:165580/1‑140 (MQ=255)                                                  gcgcCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATAc                                                                             >  2:846754/1‑140 (MQ=255)                                                    gcCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAg                                                                           >  1:1160880/1‑140 (MQ=255)                                                      cAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTg                                                                         <  2:293432/140‑1 (MQ=255)                                                      cAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTg                                                                         <  2:165580/140‑1 (MQ=255)                                                        gttgttCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt                                                                       <  2:986027/140‑1 (MQ=255)                                                        gttgttCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt                                                                       <  1:375184/140‑1 (MQ=255)                                                        gttgttCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt                                                                       <  2:24879/140‑1 (MQ=255)                                                        gttgttCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt                                                                       <  2:1160880/140‑1 (MQ=255)                                                        gttgttCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt                                                                       <  1:610698/140‑1 (MQ=255)                                                          tgttCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGt                                                                     <  1:868807/140‑1 (MQ=255)                                                             tCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTcc                                                                  <  1:629646/140‑1 (MQ=255)                                                               aTAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCa                                                                <  2:236483/140‑1 (MQ=255)                                                                tAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCaa                                                               <  2:633858/140‑1 (MQ=255)                                                                      cgcgCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt                                                                       <  1:384276/126‑1 (MQ=255)                                                                      cgcgCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt                                                                       >  2:384276/1‑126 (MQ=255)                                                                           cAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTaaa                                                    <  2:1000356/140‑1 (MQ=255)                                                                                tGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCg                                               <  2:956926/140‑1 (MQ=255)                                                                                tGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCg                                               <  1:435649/140‑1 (MQ=255)                                                                                  tCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGAt                                             <  1:53870/140‑1 (MQ=255)                                                                                            ggcggACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCa                                   >  2:359144/1‑140 (MQ=255)                                                                                                gACCGGGAACAGCAGATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGAGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGa                               >  2:1078800/1‑140 (MQ=255)                                                                                                         cagcagATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAAttg                      <  1:846754/140‑1 (MQ=255)                                                                                                           gcagATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAAttgtt                    >  1:304825/1‑140 (MQ=255)                                                                                                              gATGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCGGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCa                 <  1:91096/140‑1 (MQ=255)                                                                                                               aTGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCAt                <  2:977285/140‑1 (MQ=255)                                                                                                                tGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGaa                              >  2:838762/1‑125 (MQ=255)                                                                                                                tGACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGaa                              <  1:838762/125‑1 (MQ=255)                                                                                                                 gACCAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAg              >  2:1084791/1‑140 (MQ=255)                                                                                                                    cAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCAt                >  1:121348/1‑135 (MQ=255)                                                                                                                    cAATAAGTGTCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCAt                <  2:121348/135‑1 (MQ=255)                                                                                                                             tCAGGGCGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGa  >  1:943289/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       TGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGATGTTCCCAACATAGTGTAAATGGCGATCAGCAATGCACCGAAGGCAATTGTTGCATAGCGTTGACCCAGA  >  NC_000913/1023300‑1023563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑