Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,031,425	A→G	intergenic (‑7/+200)	yccA ← / ← serT	modulator of FtsH protease/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,031,425	0	A	G	100.0%	187.3 / NA	52	intergenic (‑7/+200)	yccA/serT	modulator of FtsH protease/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/29);  total (23/29)

TACTGGCAGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAA  >  NC_000913/1031292‑1031559                                                                                                                                      |                                                                                                                                       tACTGGCAGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGctctct                                                                                                                                  >  2:682113/1‑140 (MQ=255) tACTGGCAGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGctctct                                                                                                                                  >  1:202723/1‑140 (MQ=255) tACTGGCAGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGctctct                                                                                                                                  >  2:566340/1‑140 (MQ=255) tACTGGCAGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGctctct                                                                                                                                  >  1:93908/1‑140 (MQ=255)        aGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAg                                                                                                                           <  1:157020/140‑1 (MQ=255)        aGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAg                                                                                                                           <  2:202723/140‑1 (MQ=255)          ttGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGAt                                                                                                                         <  2:235884/140‑1 (MQ=255)             cGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAg                                                                                                                           <  2:72635/135‑1 (MQ=255)             cGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAg                                                                                                                           <  2:852554/135‑1 (MQ=255)             cGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAg                                                                                                                           >  1:852554/1‑135 (MQ=255)             cGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAg                                                                                                                           >  1:72635/1‑135 (MQ=255)                       cGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAag                                                                                                            <  2:93908/140‑1 (MQ=255)                       cGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAag                                                                                                            <  1:682113/140‑1 (MQ=255)                       cGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAag                                                                                                            <  1:68945/140‑1 (MQ=255)                       cGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAag                                                                                                            <  1:691356/140‑1 (MQ=255)                        gAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAagc                                                                                                           <  1:87945/140‑1 (MQ=255)                         aaaaGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAagca                                                                                                          >  2:417841/1‑140 (MQ=255)                          aaaGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAagcag                                                                                                         <  2:697921/140‑1 (MQ=255)                            aGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAgcagca                                                                                                       <  2:973329/140‑1 (MQ=255)                                     tCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCataatgataa                                                                                              <  2:536303/140‑1 (MQ=255)                                             agcagAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAg                                                                                      >  1:563148/1‑140 (MQ=255)                                               cagAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAggg                                                                                    <  2:563148/140‑1 (MQ=255)                                                agAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGc                                                                                   <  1:566340/140‑1 (MQ=255)                                                            ttACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAg                                                                             >  1:561952/1‑134 (MQ=255)                                                            ttACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAg                                                                             <  2:561952/134‑1 (MQ=255)                                                                gcagcaCCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTAcc                                                                   <  2:25047/140‑1 (MQ=255)                                                                  agcaCCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCa                                                                 <  1:677607/140‑1 (MQ=255)                                                                  agcaCCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCa                                                                 >  2:723097/1‑140 (MQ=255)                                                                    caCCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATc                                                               >  2:904716/1‑140 (MQ=255)                                                                              gTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCAtt                                                     <  1:723097/140‑1 (MQ=255)                                                                                 cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTAcc                                                                   <  2:966617/123‑1 (MQ=255)                                                                                 cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTAcc                                                                   >  1:966617/1‑123 (MQ=255)                                                                                 cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGa                                                  >  2:1071412/1‑140 (MQ=255)                                                                                  tAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGTCAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGAt                                                 <  2:514068/140‑1 (MQ=255)                                                                                  tAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGAt                                                 >  1:533203/1‑140 (MQ=255)                                                                                   aaGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATc                                                <  1:114844/140‑1 (MQ=255)                                                                                         tGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCAtt                                                     <  1:469434/129‑1 (MQ=255)                                                                                         tGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCAtt                                                     >  2:469434/1‑129 (MQ=255)                                                                                              tACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGtt                                     >  1:654406/1‑140 (MQ=255)                                                                                                   tCATGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTATGCATTTGATCTGGAACAGGTTTAACa                                >  2:437746/1‑140 (MQ=255)                                                                                                     aTGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                            >  2:720482/1‑126 (MQ=255)                                                                                                     aTGTGAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                            <  1:720482/126‑1 (MQ=255)                                                                                                      tgtgAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                            <  2:240786/125‑1 (MQ=255)                                                                                                      tgtgAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                            >  1:240786/1‑125 (MQ=255)                                                                                                      tgtgAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                            >  2:571757/1‑125 (MQ=255)                                                                                                      tgtgAAGAACTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                            <  1:571757/125‑1 (MQ=255)                                                                                                           agaaCTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTa                        >  1:337609/1‑140 (MQ=255)                                                                                                               cTAACAATACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAg                    >  2:983629/1‑140 (MQ=255)                                                                                                                    aaTACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCAt               <  2:572527/140‑1 (MQ=255)                                                                                                                      tACGATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTa             <  2:337609/140‑1 (MQ=255)                                                                                                                         gATCCATTGAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGc          <  1:983629/140‑1 (MQ=255)                                                                                                                                 gAGTCGCTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATaa  >  1:238991/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       TACTGGCAGTTGCGGTAATCGCCGAAAAGGCCAGCGTCAGGCTCAGCAGAAAATAGGTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAA  >  NC_000913/1031292‑1031559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑