Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,082,810	T→C	F190L (TTC→CTC)	efeO →	ferrous iron transport system protein EfeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,082,810	0	T	C	100.0%	143.2 / NA	40	F190L (TTC→CTC)	efeO	ferrous iron transport system protein EfeO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

GGCGGAAACCACGCAGCTGGTGACCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGAT  >  NC_000913/1082674‑1082941                                                                                                                                         |                                                                                                                                    ggCGGAAACCACGCAGCTGGTGACCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGctct                                                                                                                                  <  2:292027/140‑1 (MQ=255) ggCGGAAACCACGCAGCTGGTGACCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGctct                                                                                                                                  <  1:182266/140‑1 (MQ=255) ggCGGAAACCACGCAGCTGGTGACCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGctct                                                                                                                                  >  1:292027/1‑140 (MQ=255) ggCGGAAACCACGCAGCTGGTGACCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGctct                                                                                                                                  >  1:513530/1‑140 (MQ=255)                gCTGGTGACCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGgca                                                                                                                   <  2:513530/140‑1 (MQ=255)                       aCCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGa                                                                                                            <  1:970743/140‑1 (MQ=255)                         cGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGgca                                                                                                                   >  1:1031514/1‑131 (MQ=255)                         cGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGgca                                                                                                                   <  2:1031514/131‑1 (MQ=255)                                        tACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGAtt                                                                                           >  2:554671/1‑140 (MQ=255)                                             aCGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGt                                                                                                     <  1:113647/125‑1 (MQ=255)                                             aCGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGt                                                                                                     >  2:113647/1‑125 (MQ=255)                                              cgcgATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGc                                                                                     >  1:66461/1‑140 (MQ=255)                                               gcgATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCa                                                                                    >  1:826736/1‑140 (MQ=255)                                                               atatCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCa                                                                    <  2:412348/140‑1 (MQ=255)                                                                          gCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTgg                                                         >  1:1153328/1‑140 (MQ=255)                                                                          gCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTgg                                                         >  1:278080/1‑140 (MQ=255)                                                                                              gACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGt                                               <  2:1158477/130‑1 (MQ=255)                                                                                              gACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGt                                               >  1:1158477/1‑130 (MQ=255)                                                                                              gACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAg                                     <  2:278080/140‑1 (MQ=255)                                                                                                cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCa                                   >  1:851659/1‑140 (MQ=255)                                                                                                cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCa                                   >  2:760486/1‑140 (MQ=255)                                                                                                cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCa                                   >  2:683973/1‑140 (MQ=255)                                                                                                 gcgcCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCAt                                  <  2:66461/140‑1 (MQ=255)                                                                                                  cgcCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCAtt                                 >  2:72175/1‑140 (MQ=255)                                                                                                  cgcCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCAtt                                 >  2:192597/1‑140 (MQ=255)                                                                                                  cgcCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCAtt                                 >  2:26473/1‑140 (MQ=255)                                                                                                       gCACTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTg                            >  2:210997/1‑140 (MQ=255)                                                                                                         aCTATGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGc                          <  1:554671/140‑1 (MQ=255)                                                                                                             tGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGaca                      >  2:590392/1‑140 (MQ=255)                                                                                                             tGAGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGaca                      >  1:200421/1‑140 (MQ=255)                                                                                                               aGCGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGacaac                    >  1:40684/1‑140 (MQ=255)                                                                                                                 cGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAacac                  <  2:200421/140‑1 (MQ=255)                                                                                                                 cGTATTGAACCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAacac                  >  1:514069/1‑140 (MQ=255)                                                                                                                         aCCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAg          <  1:749191/140‑1 (MQ=255)                                                                                                                         aCCGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAg          >  1:415885/1‑140 (MQ=255)                                                                                                                          ccGATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAgg         <  2:819410/140‑1 (MQ=255)                                                                                                                            gATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGgga       <  1:683973/140‑1 (MQ=255)                                                                                                                            gATTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGgga       <  1:532456/140‑1 (MQ=255)                                                                                                                             aTTGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggat      >  2:305517/1‑140 (MQ=255)                                                                                                                              ttGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg     <  1:760486/140‑1 (MQ=255)                                                                                                                              ttGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg     <  1:72175/140‑1 (MQ=255)                                                                                                                              ttGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg     <  1:590392/140‑1 (MQ=255)                                                                                                                              ttGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg     <  1:305517/140‑1 (MQ=255)                                                                                                                              ttGCTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg     <  1:192597/140‑1 (MQ=255)                                                                                                                                 cTGAACTGCTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatggat  <  1:210997/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GGCGGAAACCACGCAGCTGGTGACCGACACCAAAGCCTTTACCGACGCGATTAAAGCAGGCGATATCGAAAAAGCGAAAGCACTGTATGCACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGAT  >  NC_000913/1082674‑1082941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑