Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,102,398 A→G S48S (AGT→AGC)  csgE ← curli transport specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,102,3980AG98.3% 207.3 / ‑4.3 58S48S (AGT→AGC) csgEcurli transport specificity factor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (31/26);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

TTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCG  >  NC_000913/1102268‑1102526
                                                                                                                                  |                                                                                                                                
ttttCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCt                                                                                                                         <  2:1136321/140‑1 (MQ=255)
     aaCGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTa                                                                                                                    >  1:868679/1‑140 (MQ=255)
      aCGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTaa                                                                                                                   <  1:1049159/140‑1 (MQ=255)
          aaaTAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAAt                                                                                                               >  1:292328/1‑140 (MQ=255)
           aaTAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATc                                                                                                              <  2:616604/140‑1 (MQ=255)
            aTAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCa                                                                                                             >  1:1080708/1‑140 (MQ=255)
               aaaaaGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCA‑TTATCGCTAAAGGCTCGGTAAAAATCATGGc                                                                                                         <  1:840066/140‑1 (MQ=255)
                  aaGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCa                                                                                                       <  2:83802/140‑1 (MQ=255)
                    gTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGTCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAAt                                                                                                     <  1:344167/140‑1 (MQ=255)
                    gTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAAt                                                                                                     <  1:531933/140‑1 (MQ=255)
                     tCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGc                                                                                                         >  1:139683/1‑135 (MQ=255)
                     tCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGc                                                                                                         <  2:139683/135‑1 (MQ=255)
                           aaaTAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGaa                                                                                              <  2:54445/140‑1 (MQ=255)
                              tAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACa                                                                                           >  2:972251/1‑140 (MQ=255)
                                             ccGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGc                                                                            <  2:30120/140‑1 (MQ=255)
                                               gTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCaa                                                                          >  2:343463/1‑140 (MQ=255)
                                                   taGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATccc                                                                      <  2:868679/140‑1 (MQ=255)
                                                      tGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATTGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCggg                                                                   <  1:324800/140‑1 (MQ=255)
                                                         tCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCTCTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACt                                                                <  1:870365/140‑1 (MQ=255)
                                                          ccAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACtt                                                               <  2:5942/140‑1 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGc                                                                            >  1:98087/1‑126 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGc                                                                            <  2:98087/126‑1 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCggg                                                                   >  1:57416/1‑135 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCggg                                                                   <  2:57416/135‑1 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                                              >  2:127535/1‑140 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                                              >  2:962116/1‑140 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                                              >  2:1025361/1‑140 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                                              >  1:1038432/1‑140 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                                              >  1:861255/1‑140 (MQ=255)
                                                           cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                                              >  1:30742/1‑140 (MQ=255)
                                                              cTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTAc                                                           >  2:400669/1‑140 (MQ=255)
                                                                   ccATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCaa                                                      >  1:140702/1‑140 (MQ=255)
                                                                             cTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAg                                            >  1:428192/1‑140 (MQ=255)
                                                                                ggCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCa                                                       >  1:288950/1‑126 (MQ=255)
                                                                                ggCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCa                                                       <  2:288950/126‑1 (MQ=255)
                                                                                ggCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAtt                                         >  1:1116033/1‑140 (MQ=255)
                                                                                ggCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAtt                                         >  1:197614/1‑140 (MQ=255)
                                                                                ggCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAtt                                         >  2:441372/1‑140 (MQ=255)
                                                                                          tAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTgcggcg                               <  2:861255/140‑1 (MQ=255)
                                                                                          tAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTgcggcg                               <  2:243053/140‑1 (MQ=255)
                                                                                              cGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACa                           >  1:922269/1‑140 (MQ=255)
                                                                                                 tAAGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGaa                        >  1:754149/1‑140 (MQ=255)
                                                                                                   aGTTACCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAAt                      <  1:1025361/140‑1 (MQ=255)
                                                                                                       aCCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTg                  <  2:428192/140‑1 (MQ=255)
                                                                                                       aCCCGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTg                  >  1:351549/1‑140 (MQ=255)
                                                                                                        cccGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgc                 <  1:441372/140‑1 (MQ=255)
                                                                                                        cccGTATAGTCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgc                 >  1:119018/1‑140 (MQ=255)
                                                                                                             ataGTCACTTTCCAATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCca            >  1:171888/1‑140 (MQ=255)
                                                                                                                 tCACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAAt        >  2:846330/1‑140 (MQ=255)
                                                                                                                  cACTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATc       <  1:846330/140‑1 (MQ=255)
                                                                                                                   aCTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATcc      >  2:185038/1‑140 (MQ=255)
                                                                                                                   aCTTTCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATcc      >  2:979128/1‑140 (MQ=255)
                                                                                                                      ttCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGc   <  2:197614/140‑1 (MQ=255)
                                                                                                                      ttCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGc   >  1:48295/1‑140 (MQ=255)
                                                                                                                      ttCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGc   <  1:979128/140‑1 (MQ=255)
                                                                                                                       tCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgc                 <  2:758713/125‑1 (MQ=255)
                                                                                                                       tCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgc                 >  1:758713/1‑125 (MQ=255)
                                                                                                                       tCCCATTTATCGCTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCg  >  1:764231/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                
TTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCG  >  NC_000913/1102268‑1102526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑