Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,214,789	T→C	A229A (GCA→GCG)	bluF ←	blue light‑responsive regulator of BluR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,214,789	0	T	C	100.0%	181.9 / NA	50	A229A (GCA→GCG)	bluF	blue light‑responsive regulator of BluR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/22);  total (28/22)

CAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGC  >  NC_000913/1214653‑1214924                                                                                                                                         |                                                                                                                                        cAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGcc                                                                                                                                      <  2:120621/140‑1 (MQ=255)  aaCGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCa                                                                                                                                     >  1:754005/1‑140 (MQ=255)   aCGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCAt                                                                                                                                    >  2:562792/1‑140 (MQ=255)         atTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGCCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGaa                                                                                                                              >  2:133218/1‑140 (MQ=255)                        agagCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACt                                                                                                               >  2:97354/1‑140 (MQ=255)                           gCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACtttt                                                                                                            <  2:576165/140‑1 (MQ=255)                            cATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCGAGCGCCTTACTTTTg                                                                                                           >  2:1056817/1‑140 (MQ=255)                                ggCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGAt                                                                                                       <  2:754005/140‑1 (MQ=255)                                   cTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCg                                                                                                    <  1:1032883/140‑1 (MQ=255)                                    tttATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGc                                                                                                   >  2:590731/1‑140 (MQ=255)                                      tATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCtg                                                                                                 >  2:695837/1‑140 (MQ=255)                                                  tAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTccc                                                                                     <  2:140377/140‑1 (MQ=255)                                                  tAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTccc                                                                                     <  2:936236/140‑1 (MQ=255)                                                  tAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTccc                                                                                     <  1:764874/140‑1 (MQ=255)                                                   aaaaaaGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTcccc                                                                                    >  2:1022730/1‑140 (MQ=255)                                                            aCTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACg                                                                           >  2:425226/1‑140 (MQ=255)                                                            aCTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACg                                                                           <  1:981873/140‑1 (MQ=255)                                                                   cAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCa                                                                    <  2:270902/140‑1 (MQ=255)                                                                   cAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCACGTCTTTACGCTGCCCa                                                                    <  1:97354/140‑1 (MQ=255)                                                                    aGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCaa                                                                   <  1:425226/140‑1 (MQ=255)                                                                      gTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAAcc                                                                 >  1:906475/1‑140 (MQ=255)                                                                           ttAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTAt                                                            <  1:757995/140‑1 (MQ=255)                                                                                  gggTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCGAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGAt                                                     <  1:1056817/140‑1 (MQ=255)                                                                                   ggTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATg                                                    >  1:144066/1‑140 (MQ=255)                                                                                        tAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTa                                               >  2:851212/1‑140 (MQ=255)                                                                                             aaTAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTc                                          >  1:1134233/1‑140 (MQ=255)                                                                                             aaTAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTc                                          >  2:893374/1‑140 (MQ=255)                                                                                               tAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAt                                        >  1:139563/1‑140 (MQ=255)                                                                                               tAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAt                                        >  2:858461/1‑140 (MQ=255)                                                                                               tAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAt                                        >  2:863778/1‑140 (MQ=255)                                                                                                    tgattgaAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAtttttt                                   >  2:570308/1‑140 (MQ=255)                                                                                                     gattgaAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAttttttt                                  >  1:20994/1‑140 (MQ=255)                                                                                                     gattgaAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAttttttt                                  >  1:725055/1‑140 (MQ=255)                                                                                                      attgaAATCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTg                                 >  1:46623/1‑140 (MQ=255)                                                                                                          aaaTCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCTCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGcaca                             <  1:153293/140‑1 (MQ=255)                                                                                                          aaaTCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGcaca                             <  2:1134233/140‑1 (MQ=255)                                                                                                          aaaTCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGcaca                             <  1:695837/140‑1 (MQ=255)                                                                                                          aaaTCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGcaca                             <  1:893374/140‑1 (MQ=255)                                                                                                            aTCATTTTATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAAt                           <  2:996046/140‑1 (MQ=255)                                                                                                                ttttATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCt                       <  1:635078/140‑1 (MQ=255)                                                                                                                 tttATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATgg                         <  2:202396/137‑1 (MQ=255)                                                                                                                 tttATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATgg                         >  1:202396/1‑137 (MQ=255)                                                                                                                 tttATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTACGGCTGATGGGCTATCTTCATTTTTTTGCACAATgg                         >  1:783835/1‑137 (MQ=255)                                                                                                                 tttATCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTACGGCTGATGGGCTATCTTCATTTTTTTGCACAATgg                         <  2:783835/137‑1 (MQ=255)                                                                                                                     tCACCGAGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCaaa                  >  2:306118/1‑140 (MQ=255)                                                                                                                           aGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGAt            >  1:629418/1‑140 (MQ=255)                                                                                                                           aGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGAt            >  1:162991/1‑140 (MQ=255)                                                                                                                           aGCTCAAGTGCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGAt            >  1:1003359/1‑140 (MQ=255)                                                                                                                                  gtgcgtgcGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATccgc     <  2:725055/140‑1 (MQ=255)                                                                                                                                  gtgcgtgcGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTAAccgc     <  1:306118/140‑1 (MQ=255)                                                                                                                                    gCGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATccgccg   <  1:1022730/140‑1 (MQ=255)                                                                                                                                     cGTGCGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCcgccgc  >  2:145037/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTTTTGCACAATGGCTTCAAAAGCGATTATCCGCCGC  >  NC_000913/1214653‑1214924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑