Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,280,679	T→C	R272R (CGT→CGC)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,280,679	0	T	C	100.0%	182.6 / NA	50	R272R (CGT→CGC)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/27);  total (23/27)

CTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAAT  >  NC_000913/1280546‑1280815                                                                                                                                      |                                                                                                                                         ctcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACaaa                                                                                                                                    <  2:346884/140‑1 (MQ=255) ctcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACaaa                                                                                                                                    <  1:902250/140‑1 (MQ=255)  tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAg                                                                                                                                   <  1:513565/140‑1 (MQ=255)    cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAggg                                                                                                                                 >  2:627981/1‑140 (MQ=255)      cAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGAc                                                                                                                               >  1:354600/1‑140 (MQ=255)                gCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACACCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGtt                                                                                                                     <  2:722138/140‑1 (MQ=255)                   aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGt                                                                                                                      >  2:752722/1‑136 (MQ=255)                   aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGt                                                                                                                      <  1:752722/136‑1 (MQ=255)                   aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGcc                                                                                                                  >  1:229356/1‑140 (MQ=255)                   aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGcc                                                                                                                  >  2:678923/1‑140 (MQ=255)                   aGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGcc                                                                                                                  >  1:708377/1‑140 (MQ=255)                    gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCg                                                                                                                 <  2:355662/140‑1 (MQ=255)                    gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCg                                                                                                                 <  2:922329/140‑1 (MQ=255)                     cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGt                                                                                                                <  2:320956/140‑1 (MQ=255)                     cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGt                                                                                                                <  2:229356/140‑1 (MQ=255)                     cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGt                                                                                                                <  2:1157373/140‑1 (MQ=255)                         cTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTcaca                                                                                                            <  2:708377/140‑1 (MQ=255)                           gACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACAcc                                                                                                          <  1:198432/140‑1 (MQ=255)                                aCCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACt                                                                                                     <  2:354600/140‑1 (MQ=255)                                      aTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTg                                                                                               >  1:636399/1‑140 (MQ=255)                                                   tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACt                                                                                  <  1:292290/140‑1 (MQ=255)                                                    aCAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtg                                                                                 <  2:114489/140‑1 (MQ=255)                                                        cTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGcc                                                                                       <  1:226051/130‑1 (MQ=255)                                                        cTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGcc                                                                                       >  2:226051/1‑130 (MQ=255)                                                                  atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggct                                                                   <  2:45936/140‑1 (MQ=255)                                                                  atcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggct                                                                   >  1:45936/1‑140 (MQ=255)                                                                   tcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctg                                                                  >  1:763948/1‑140 (MQ=255)                                                                            gggggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa                                                         <  2:636399/140‑1 (MQ=255)                                                                              gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACa                                                       <  1:157287/140‑1 (MQ=255)                                                                                  cAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGc                                                   >  1:532351/1‑140 (MQ=255)                                                                                  cAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGc                                                   >  2:169686/1‑140 (MQ=255)                                                                                    aaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAc                                                 >  1:1075111/1‑140 (MQ=255)                                                                                          ccGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGc                                           >  2:1144269/1‑140 (MQ=255)                                                                                               gACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCaa                                      <  1:678923/140‑1 (MQ=255)                                                                                                    gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg                                 >  2:1006528/1‑140 (MQ=255)                                                                                                     tACCCCGGATGCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcgc                                >  2:944637/1‑140 (MQ=255)                                                                                                               gCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                      <  2:334612/140‑1 (MQ=255)                                                                                                               gCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                      <  2:1158867/140‑1 (MQ=255)                                                                                                               gCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                      <  1:944637/140‑1 (MQ=255)                                                                                                               gCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                      <  1:542413/140‑1 (MQ=255)                                                                                                               gCTCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                      <  1:1144269/140‑1 (MQ=255)                                                                                                                 tCACTTCTTTACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGcc                    >  1:326474/1‑140 (MQ=255)                                                                                                                          tACTGAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgc           >  1:374152/1‑140 (MQ=255)                                                                                                                              gAAGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCg       <  1:976041/140‑1 (MQ=255)                                                                                                                               aaGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                      <  1:71973/124‑1 (MQ=255)                                                                                                                               aaGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg                      >  2:71973/1‑124 (MQ=255)                                                                                                                               aaGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt      >  1:737585/1‑140 (MQ=255)                                                                                                                               aaGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt      >  2:645965/1‑140 (MQ=255)                                                                                                                               aaGTGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt      >  1:555075/1‑140 (MQ=255)                                                                                                                                  tGCGCTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGaa   >  2:244425/1‑140 (MQ=255)                                                                                                                                   gcgcTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAAt  >  2:1092613/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAAT  >  NC_000913/1280546‑1280815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑