Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,363,075	A→G	K111K (AAA→AAG)	puuC →	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,363,075	0	A	G	100.0%	102.5 / NA	29	K111K (AAA→AAG)	puuC	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

GTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAACCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGA  >  NC_000913/1362947‑1363209                                                                                                                                 |                                                                                                                                       gTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTca                                                                                                                             <  2:686785/140‑1 (MQ=255)  tATTTGAACGCGGCGACTGGTCACTCTCTTCACCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTcac                                                                                                                            <  2:242940/140‑1 (MQ=255)     ttGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGt                                                                                                                         <  1:892742/140‑1 (MQ=255)            cggcgACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGtg                                                                                                                  >  1:519244/1‑140 (MQ=255)             ggcgACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGt                                                                                                                         >  2:280997/1‑132 (MQ=255)             ggcgACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGt                                                                                                                         <  1:280997/132‑1 (MQ=255)               cgACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGtgatg                                                                                                               >  1:925237/1‑140 (MQ=255)                     gTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTg                                                                                                                      <  1:281731/127‑1 (MQ=255)                     gTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTg                                                                                                                      >  2:281731/1‑127 (MQ=255)                         ctctctTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCcgg                                                                                                     >  2:54714/1‑140 (MQ=255)                                        aaaCGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCAt                                                                                      >  1:500524/1‑140 (MQ=255)                                              aaaGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTg                                                                                <  2:39902/140‑1 (MQ=255)                                                     tACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGcc                                                                         >  2:829514/1‑140 (MQ=255)                                                      aCTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCg                                                                        >  1:665413/1‑140 (MQ=255)                                                      aCTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCg                                                                        >  1:957043/1‑140 (MQ=255)                                                            tAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGa                                                                  >  1:698791/1‑140 (MQ=255)                                                                    ccGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACaaa                                                          <  2:794842/140‑1 (MQ=255)                                                                    ccGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACaaa                                                          >  2:605209/1‑140 (MQ=255)                                                                    ccGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGCCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACaaa                                                          >  1:1052600/1‑140 (MQ=255)                                                                      gATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAgt                                                        <  2:774499/140‑1 (MQ=255)                                                                                      aCGCCGAAGAGCTGGCACTGCTGGAAACTCTCGCCACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCg                                        <  2:925237/140‑1 (MQ=255)                                                                                       cGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGa                                       >  1:154091/1‑140 (MQ=255)                                                                                           gAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGaccac                                   <  2:698791/140‑1 (MQ=255)                                                                                                   ggCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCg                                        <  2:1145327/127‑1 (MQ=255)                                                                                                   ggCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCg                                        >  1:1145327/1‑127 (MQ=255)                                                                                                    gCACTGCTGGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCa                          <  2:154091/140‑1 (MQ=255)                                                                                                        tgctgGAAACTCTCGACACCGGCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAg                      <  2:519244/140‑1 (MQ=255)                                                                                                                            ggCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGa  <  1:919381/140‑1 (MQ=255)                                                                                                                            ggCAAGCCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGa  >  1:1143396/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       GTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGAAGAGCTGGCACTGCTGGAAACTCTCGACACCGGCAAACCGATTCGTCACAGTCTGCGTGATGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGA  >  NC_000913/1362947‑1363209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑