| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 79,924 | A→G | V314A (GTT→GCT) | leuC ← | 3‑isopropylmalate dehydratase subunit LeuC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 79,924 | 0 | A | G | 97.3% | 125.0 / ‑4.1 | 37 | V314A (GTT→GCT) | leuC | 3‑isopropylmalate dehydratase subunit LeuC |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (20/16); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTT > NC_000913/79793‑80059 | ccGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCg > 2:947263/1‑140 (MQ=255) tCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCgg < 1:419582/140‑1 (MQ=255) aaTGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGc < 1:1151435/135‑1 (MQ=255) aaTGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGc > 2:1151435/1‑135 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCggg > 1:673997/1‑140 (MQ=255) gcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGa < 2:558395/140‑1 (MQ=255) ggTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGAt > 2:819127/1‑128 (MQ=255) ggTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGAt < 1:819127/128‑1 (MQ=255) gAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCAc > 2:72906/1‑140 (MQ=255) gAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCAc > 1:906278/1‑140 (MQ=255) aCCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACgg > 2:454295/1‑140 (MQ=255) aCCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACgg > 2:126065/1‑140 (MQ=255) aTAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAAt < 1:454295/140‑1 (MQ=255) acacTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCAcc < 2:673997/140‑1 (MQ=255) cacTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCt < 1:546529/140‑1 (MQ=255) tGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCg > 2:934717/1‑140 (MQ=255) tGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCg > 1:788196/1‑140 (MQ=255) aTAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGAtt < 2:990652/140‑1 (MQ=255) cACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGc > 1:142345/1‑140 (MQ=255) cACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGc < 2:788196/140‑1 (MQ=255) cTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGccc > 1:564423/1‑140 (MQ=255) tCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTg > 1:144113/1‑140 (MQ=255) ggAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTg < 1:934717/140‑1 (MQ=255) aaTACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCg > 1:10945/1‑140 (MQ=255) tACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGt < 2:10945/140‑1 (MQ=255) ttCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATttc > 1:501070/1‑140 (MQ=255) cAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATttctt > 2:58108/1‑140 (MQ=255) atatAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTg < 2:501070/140‑1 (MQ=255) gCCAGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGt < 1:947263/140‑1 (MQ=255) aGCGCTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGAc < 2:906278/140‑1 (MQ=255) cgcTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACaa > 2:691118/1‑140 (MQ=255) cgcTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACaa > 2:655271/1‑140 (MQ=255) cgcTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACaa < 1:58108/140‑1 (MQ=255) cgcTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACaa > 2:557584/1‑140 (MQ=255) cgcTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACaa > 1:47049/1‑140 (MQ=255) cTTTTTCTGCCGACGCGCGTTCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACg < 1:655271/140‑1 (MQ=255) ttCAGCCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCtt > 2:247854/1‑140 (MQ=255) | CCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTGCGGTGAAATTTCTTCTGCTTGCAGAGTGACAACGGTATCGAAAGTTGCGCCTT > NC_000913/79793‑80059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |