Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,442,442	C→T	L134L (CTG→CTA)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,442,442	0	C	T	100.0%	216.3 / NA	60	L134L (CTG→CTA)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/33);  total (27/33)

CTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCC  >  NC_000913/1442306‑1442574                                                                                                                                         |                                                                                                                                     cTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTaga                                                                                                                                   <  1:780005/140‑1 (MQ=255)  tGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTagag                                                                                                                                  <  1:792463/140‑1 (MQ=255)              cGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCAt                                                                                                                      <  2:104213/140‑1 (MQ=255)              cGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCAt                                                                                                                      >  1:479977/1‑140 (MQ=255)                 aTCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCTTACTTATAAAAGCCGGCCAAACCTTCTAGAGAGAATTAAGCATCag                                                                                                                   >  1:247461/1‑139 (MQ=255)                 aTCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCAc                                                                                                                   >  2:610211/1‑140 (MQ=255)                 aTCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCAc                                                                                                                   >  2:664886/1‑140 (MQ=255)                   caaaCGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACgg                                                                                                                 <  1:1144921/138‑1 (MQ=255)                   cGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACgg                                                                                                                 <  1:837115/140‑1 (MQ=255)                     aaCGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACggg                                                                                                                >  2:1050012/1‑139 (MQ=255)                     aaCGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACggg                                                                                                                <  1:1050012/139‑1 (MQ=255)                     aaCGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGt                                                                                                               >  2:726877/1‑140 (MQ=255)                     aaCGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGt                                                                                                               <  1:467831/140‑1 (MQ=255)                           agcagACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTg                                                                                                         >  2:564442/1‑140 (MQ=255)                                aCGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCAt                                                                                                                      >  2:107560/1‑122 (MQ=255)                                aCGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCAt                                                                                                                      >  2:1071402/1‑122 (MQ=255)                                aCGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCAt                                                                                                                      <  1:1071402/122‑1 (MQ=255)                                aCGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCAt                                                                                                                      <  1:107560/122‑1 (MQ=255)                                         aaaaCCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGaa                                                                                           <  2:12965/140‑1 (MQ=255)                                            aCCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATAc                                                                                        >  1:555223/1‑140 (MQ=255)                                                ttCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATAcggcg                                                                                    >  1:1065602/1‑140 (MQ=255)                                                   aGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGtt                                                                                 <  1:805737/140‑1 (MQ=255)                                                     aaTGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCa                                                                               <  2:187499/140‑1 (MQ=255)                                                       tGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGc                                                                             >  2:114805/1‑140 (MQ=255)                                                         cgcAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGt                                                                           <  2:555223/140‑1 (MQ=255)                                                         cgcAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGt                                                                           <  1:664886/140‑1 (MQ=255)                                                         cgcAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGt                                                                           <  1:114805/140‑1 (MQ=255)                                                           cagcaTCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGtca                                                                         <  2:83466/140‑1 (MQ=255)                                                             gcaTCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGtcatc                                                                       <  1:168235/140‑1 (MQ=255)                                                                 cGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGtcatcatca                                                                   >  2:396945/1‑140 (MQ=255)                                                                 cGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGtcatcatca                                                                   <  1:396945/140‑1 (MQ=255)                                                                   ccACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATa                                                                 >  1:406837/1‑140 (MQ=255)                                                                      cacCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCg                                                              >  2:380679/1‑140 (MQ=255)                                                                         cGATTTTACCGGTACCGATAACGCCTGCAGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATg                                                           <  1:106812/140‑1 (MQ=255)                                                                                       ccGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAAc                                             <  1:1094906/140‑1 (MQ=255)                                                                                       ccGATAACGCCTGCCGTTTTGCCATACATACTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAAc                                             <  1:606965/140‑1 (MQ=255)                                                                                        cGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACg                                            <  2:406837/140‑1 (MQ=255)                                                                                        cGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACg                                            <  2:682697/140‑1 (MQ=255)                                                                                          aTAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGc                                          >  1:889569/1‑140 (MQ=255)                                                                                          aTAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGc                                          <  1:1106847/140‑1 (MQ=255)                                                                                          aTAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGAAGGGGCGGTCAGCaaacgc                                          >  1:1148031/1‑136 (MQ=255)                                                                                            aaCGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCct                                        <  2:106830/140‑1 (MQ=255)                                                                                              cGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCctct                                      <  1:286911/140‑1 (MQ=255)                                                                                               gCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGGCATCATCATACCCATGGCGCGTTCCGCAACGGCCCCTg                                     >  2:319739/1‑140 (MQ=255)                                                                                                  tGCCGTTTTGCCATACTTAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGAt                                  >  1:390746/1‑140 (MQ=255)                                                                                                  tGCCGTTTTGCCATACATACTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCCGCGTCATCCTCTTACCCCTGGCGCGGTCAGCAACGGcccccgggt                                  >  2:606965/1‑133 (MQ=255)                                                                                                    ccGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCa                                <  1:235552/140‑1 (MQ=255)                                                                                                               tacataGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAac                     <  2:889569/140‑1 (MQ=255)                                                                                                                acataGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAaca                    <  1:610211/140‑1 (MQ=255)                                                                                                                 cataGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCCATGGCGCGGTCAGCAACGGCCTCTGGGTCATAGGCTgggaaac                   >  2:199770/1‑135 (MQ=255)                                                                                                                 agtaGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAacac                   <  1:969690/138‑1 (MQ=255)                                                                                                                           ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTTTGCGGCATCATCAAACCGAGGGCGCGGTTAGCCACGGCCCCTGGGTCATAGGCTGGAACACGGACCACttt         >  2:968334/1‑140 (MQ=255)                                                                                                                           ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCCGCGGCATCATCATACCCAAGGCGCGGTCAGCAACGGCCTCCTGGTCATAAGCTGGAACACGGGCTACttt         >  2:134546/1‑140 (MQ=255)                                                                                                                           ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACttt         >  2:230974/1‑140 (MQ=255)                                                                                                                           ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCCATGGCGCGGTCAGCAACGGCCCCCGGGTCATAAGCTGGGACACCGACCACttt         >  2:1130149/1‑140 (MQ=255)                                                                                                                           ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGGCATCATCATACCCATGGCGCGGTCCGCAACGGCCCCTGGATCATAAGCTGGGAAAACGACTACttt         >  2:561829/1‑140 (MQ=255)                                                                                                                           ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAACGTCACCATACTACCGAGGGCGCGGTCAGCAAAGGCCCCTGGATGCTAAGCTGGAAAACGGACGAAttt         >  2:358347/1‑140 (MQ=255)                                                                                                                            cGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTc        <  2:1065602/140‑1 (MQ=255)                                                                                                                            cGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTc        <  1:358347/140‑1 (MQ=255)                                                                                                                              gTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGCCATCATCATACCGCGGGCGCGGTCAGCGACGGCCTCTGGATCATAGGCTGGAAAACGGCCTACTTTccc      >  2:969690/1‑138 (MQ=255)                                                                                                                                  gACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGcccc  <  1:380679/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCC  >  NC_000913/1442306‑1442574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑