Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,442,521	T→C	H108R (CAC→CGC)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,442,521	0	T	C	100.0%	133.4 / NA	37	H108R (CAC→CGC)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/31);  total (6/31)

CCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGC  >  NC_000913/1442392‑1442655                                                                                                                                  |                                                                                                                                        ccGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAAc                                                                                                                               <  1:1094906/140‑1 (MQ=255) ccGATAACGCCTGCCGTTTTGCCATACATACTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAAc                                                                                                                               <  1:606965/140‑1 (MQ=255)  cGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACg                                                                                                                              <  2:682697/140‑1 (MQ=255)  cGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACg                                                                                                                              <  2:406837/140‑1 (MQ=255)    aTAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGc                                                                                                                            <  1:1106847/140‑1 (MQ=255)    aTAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGc                                                                                                                            >  1:889569/1‑140 (MQ=255)    aTAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGAAGGGGCGGTCAGCaaacgc                                                                                                                            >  1:1148031/1‑136 (MQ=255)      aaCGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCct                                                                                                                          <  2:106830/140‑1 (MQ=255)        cGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCctct                                                                                                                        <  1:286911/140‑1 (MQ=255)         gCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGGCATCATCATACCCATGGCGCGTTCCGCAACGGCCCCTg                                                                                                                       >  2:319739/1‑140 (MQ=255)            tGCCGTTTTGCCATACTTAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGAt                                                                                                                    >  1:390746/1‑140 (MQ=255)            tGCCGTTTTGCCATACATACTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCCGCGTCATCCTCTTACCCCTGGCGCGGTCAGCAACGGcccccgggt                                                                                                                    >  2:606965/1‑133 (MQ=255)              ccGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCa                                                                                                                  <  1:235552/140‑1 (MQ=255)                         tacataGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAac                                                                                                       <  2:889569/140‑1 (MQ=255)                          acataGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAaca                                                                                                      <  1:610211/140‑1 (MQ=255)                           cataGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCCATGGCGCGGTCAGCAACGGCCTCTGGGTCATAGGCTgggaaac                                                                                                     >  2:199770/1‑135 (MQ=255)                           agtaGTAAAGCCGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAacac                                                                                                     <  1:969690/138‑1 (MQ=255)                                     ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTTTGCGGCATCATCAAACCGAGGGCGCGGTTAGCCACGGCCCCTGGGTCATAGGCTGGAACACGGACCACttt                                                                                           >  2:968334/1‑140 (MQ=255)                                     ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCCGCGGCATCATCATACCCAAGGCGCGGTCAGCAACGGCCTCCTGGTCATAAGCTGGAACACGGGCTACttt                                                                                           >  2:134546/1‑140 (MQ=255)                                     ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACttt                                                                                           >  2:230974/1‑140 (MQ=255)                                     ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCCATGGCGCGGTCAGCAACGGCCCCCGGGTCATAAGCTGGGACACCGACCACttt                                                                                           >  2:1130149/1‑140 (MQ=255)                                     ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGGCATCATCATACCCATGGCGCGGTCCGCAACGGCCCCTGGATCATAAGCTGGGAAAACGACTACttt                                                                                           >  2:561829/1‑140 (MQ=255)                                     ccGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAACGTCACCATACTACCGAGGGCGCGGTCAGCAAAGGCCCCTGGATGCTAAGCTGGAAAACGGACGAAttt                                                                                           >  2:358347/1‑140 (MQ=255)                                      cGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTc                                                                                          <  2:1065602/140‑1 (MQ=255)                                      cGGTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTc                                                                                          <  1:358347/140‑1 (MQ=255)                                        gTCAGACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGCCATCATCATACCGCGGGCGCGGTCAGCGACGGCCTCTGGATCATAGGCTGGAAAACGGCCTACTTTccc                                                                                        >  2:969690/1‑138 (MQ=255)                                            gACCTTCTAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGcccc                                                                                    <  1:380679/140‑1 (MQ=255)                                                  ctagagagAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCt                                                                              >  2:1048270/3‑140 (MQ=255)                                                       gagaAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGc                                                                         <  1:968334/140‑1 (MQ=255)                                                          aaGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGGTCAGCAACGGCCTCTGGATCATAGGCTGGGACACGGACTACTTTCAGCCCCAGGTCttttttcgc                                                                      >  2:638413/1‑135 (MQ=255)                                                          aaGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGGTCAGCAAAGGCCTCTGGGTCATAGGCTGGGAAACGGACTACTTTTAGCCCCCGTTCTTTTTCc‑gc                                                                     >  2:335884/1‑140 (MQ=255)                                                          aaGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGGCATCATCATACCCATGGCGCGGTCAGCAACGGCCTCTGGGTCATAGGCTGGGACACGGACTACTTTCAGCCCCAGTTCTTTTTCc‑gc                                                                     >  2:1075137/1‑140 (MQ=255)                                                               aGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGGCATCATCATACCCATGGCGCGGTCAGCAACGGCCCCTGGGTCATAGGCTGGGACACGGACTACTTTTAGCCCCCCTTCTTTTTCC‑GCGTcca                                                                >  2:1090633/1‑138 (MQ=255)                                                                       gggTACGCTGATACGCGCGGTGAATACGGCGGTTTTGCCGCCTCATCATACCCAAGGCGCGGTTAGCAAAGGCCCCTGGATCCTAGGGCGGGACACGGGCCACCTTCAGCCCCACGTCTTTTTGC‑GCGGCAAggggccag                                                        >  2:492295/1‑134 (MQ=255)                                                                            cGCTGATACGCGCGGTGAATACGGCGGTTTAGCGTCCTCATCATACCGCTGGGGCGGTCAGCAACGGCCCCTGGGTCATAAGGTGGGACACGGAATACTTTCAGCCCCCGTTCTTTTTCC‑GCGTCCAGGGCGGCGGTAtt                                                   >  2:819603/1‑140 (MQ=255)                                                                            cGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTAtt                                                   <  1:1048270/140‑1 (MQ=255)                                                                            cGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTAtt                                                   <  1:1090633/140‑1 (MQ=255)                                                                                 aTACGCGCGGTGAATACGGCGGTTCCGCGTCATCATCCTACCGGAGGCGCGGTCAGCAAAGGGCCCTGGGTTATAAGGTGGGAAACCGGCTACTTTTCGCCCCCCGTCTTTTTCCTCCGCCAAGG‑CGACGGTATTTaaaa                                              >  2:1139722/1‑139 (MQ=255)                                                                                      cgcgGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGca                                         <  1:120486/140‑1 (MQ=255)                                                                                             aaTACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCa                                  <  1:819603/140‑1 (MQ=255)                                                                                             aaTACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCa                                  <  1:1139722/140‑1 (MQ=255)                                                                                             aaTACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCa                                  <  1:230974/140‑1 (MQ=255)                                                                                               tACGGCGGTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAgg                                <  1:1075137/140‑1 (MQ=255)                                                                                                  ggcggTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCg                             <  1:561829/140‑1 (MQ=255)                                                                                                  ggcggTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCg                             <  2:1148031/140‑1 (MQ=255)                                                                                                    cggTTCAGCGTCATCATCATACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGat                           <  1:335884/140‑1 (MQ=255)                                                                                                                 tcatcatACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATAttt                     <  2:493293/133‑1 (MQ=255)                                                                                                                 tcatcatACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATAttt                     <  2:532709/133‑1 (MQ=255)                                                                                                                 tcatcatACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATAttt                     >  1:493293/1‑133 (MQ=255)                                                                                                                 tcatcatACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATAttt                     >  1:532709/1‑133 (MQ=255)                                                                                                                      atACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCtt         >  2:626804/1‑140 (MQ=255)                                                                                                                      atACCGATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCtt         >  2:630359/1‑140 (MQ=255)                                                                                                                           gATGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCa    <  1:134546/140‑1 (MQ=255)                                                                                                                            aTGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAg   >  1:360724/1‑140 (MQ=255)                                                                                                                            aTGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAg   >  1:455055/1‑140 (MQ=255)                                                                                                                             tGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGc  <  2:390746/140‑1 (MQ=255)                                                                                                                             tGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGc  <  1:564442/140‑1 (MQ=255)                                                                                                                             tGGCGCGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGc  <  1:626804/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        CCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACGGGTACGCTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCC‑GCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGC  >  NC_000913/1442392‑1442655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑