Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,522,316	A→G	G250G (GGT→GGC)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,522,316	0	A	G	100.0%	274.8 / NA	74	G250G (GGT→GGC)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/37);  total (37/37)

TCATATCATCCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGT  >  NC_000913/1522180‑1522447                                                                                                                                         |                                                                                                                                    tCATATCATCCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCt                                                                                                                                  >  1:1060470/1‑140 (MQ=255) tCATATCATCCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCt                                                                                                                                  >  2:554277/1‑140 (MQ=255) tCATATCATCCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCt                                                                                                                                  >  2:438945/1‑140 (MQ=255)   atatCATCCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTgg                                                                                                                                >  2:818928/1‑140 (MQ=255)        atcCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCAt                                                                                                                           <  2:662694/140‑1 (MQ=255)         tcCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATc                                                                                                                          >  2:1138204/1‑140 (MQ=255)         tcCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATc                                                                                                                          >  2:892881/1‑140 (MQ=255)         tcCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATc                                                                                                                          >  1:185873/1‑140 (MQ=255)         tcCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATc                                                                                                                          >  1:647291/1‑140 (MQ=255)         tcCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATc                                                                                                                          >  2:265216/1‑140 (MQ=255)         tcCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATc                                                                                                                          >  2:516251/1‑140 (MQ=255)             gcgAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCt                                                                                                                      >  1:761324/1‑140 (MQ=255)             gcgAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCt                                                                                                                      >  2:212057/1‑140 (MQ=255)             gcgAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCt                                                                                                                      >  1:622448/1‑140 (MQ=255)              cgAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCtt                                                                                                                     >  2:1145869/1‑140 (MQ=255)               gAACTCAGGCTATGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCttt                                                                                                                    <  2:36595/140‑1 (MQ=255)               gAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCGCGAGCCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCttt                                                                                                                    <  1:305188/140‑1 (MQ=255)               gAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCttt                                                                                                                    <  1:1145869/140‑1 (MQ=255)               gAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCttt                                                                                                                    <  1:265216/140‑1 (MQ=255)                aaCTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTg                                                                                                                   <  1:1138204/140‑1 (MQ=255)                aaCTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTg                                                                                                                   <  1:32577/140‑1 (MQ=255)                  cTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGat                                                                                                                 <  1:704407/140‑1 (MQ=255)                     aGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATc                                                                                                              <  2:264742/140‑1 (MQ=255)                       gCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCca                                                                                                            <  1:516251/140‑1 (MQ=255)                       gCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCca                                                                                                            <  2:185873/140‑1 (MQ=255)                       gCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCca                                                                                                            <  1:134058/140‑1 (MQ=255)                         tACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCcaca                                                                                                          <  1:465362/140‑1 (MQ=255)                         tACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCcaca                                                                                                          <  2:318468/140‑1 (MQ=255)                         tACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCcaca                                                                                                          <  2:420025/140‑1 (MQ=255)                           cGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCca                                                                                                            >  1:630682/1‑136 (MQ=255)                           cGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCca                                                                                                            <  2:630682/136‑1 (MQ=255)                           cGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACt                                                                                                        >  2:147126/1‑140 (MQ=255)                             cTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTg                                                                                                                   <  2:1102156/127‑1 (MQ=255)                             cTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTg                                                                                                                   >  1:1102156/1‑127 (MQ=255)                                 tAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGaa                                                                                                  >  1:661606/1‑140 (MQ=255)                                 tAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGaa                                                                                                  >  2:169017/1‑140 (MQ=255)                                      cAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAAt                                                                                               >  1:126146/1‑138 (MQ=255)                                      cAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAAt                                                                                               <  2:126146/138‑1 (MQ=255)                                      cAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCa                                                                                             >  2:684703/1‑140 (MQ=255)                                       aaCCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAg                                                                                            <  1:818928/140‑1 (MQ=255)                                        aCCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGa                                                                                           >  2:334717/1‑140 (MQ=255)                                                 gAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTg                                                                                  <  2:661606/140‑1 (MQ=255)                                                   gTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCt                                                                                >  1:333322/1‑140 (MQ=255)                                                      tGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGc                                                                             <  2:187933/140‑1 (MQ=255)                                                        cTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCtt                                                                           >  1:173121/1‑140 (MQ=255)                                                              ggTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCaa                                                                     <  2:52023/140‑1 (MQ=255)                                                                        gtgtCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGcc                                                           <  1:438945/140‑1 (MQ=255)                                                                        gtgtCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGcc                                                           <  2:1060470/140‑1 (MQ=255)                                                                             gTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGtt                                                      <  2:173121/140‑1 (MQ=255)                                                                                  gTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCa                                                 >  1:368591/1‑140 (MQ=255)                                                                                    tCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCAtt                                               >  1:409558/1‑140 (MQ=255)                                                                                     cTGCACGAAGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTg                                              >  1:915540/1‑140 (MQ=255)                                                                                      tGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGc                                                  <  1:415521/135‑1 (MQ=255)                                                                                      tGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGc                                                  >  2:415521/1‑135 (MQ=255)                                                                                        cACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCt                                           >  2:748133/1‑140 (MQ=255)                                                                                             ggCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAAtt                                      >  2:538418/1‑140 (MQ=255)                                                                                             ggCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAAtt                                      >  2:387486/1‑140 (MQ=255)                                                                                             ggCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAAtt                                      >  2:154998/1‑140 (MQ=255)                                                                                             ggCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAAAGCGTACGCCCAGTTTGGCATTGGCTAAAtt                                      >  1:685481/1‑140 (MQ=255)                                                                                                  ttgttgTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCt                                 <  1:334717/140‑1 (MQ=255)                                                                                                  ttgttgTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCt                                 <  1:892881/140‑1 (MQ=255)                                                                                                  ttgttgTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCt                                 <  2:1160827/140‑1 (MQ=255)                                                                                                        tgGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTgcgc                           <  1:147126/140‑1 (MQ=255)                                                                                                        tgGATTAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTgcgc                           <  1:684703/140‑1 (MQ=255)                                                                                                            ttAGCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACt                       <  2:409558/140‑1 (MQ=255)                                                                                                               gCCTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATg                    >  2:659574/1‑140 (MQ=255)                                                                                                                 cTTCCATTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAAAGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGt                  <  2:685481/140‑1 (MQ=255)                                                                                                                      aTTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGa             <  2:647291/140‑1 (MQ=255)                                                                                                                      aTTCTGCTTTGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGa             <  2:622448/140‑1 (MQ=255)                                                                                                                             tttGGTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGt      <  1:659574/140‑1 (MQ=255)                                                                                                                                 gTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGt  >  2:879609/1‑140 (MQ=255)                                                                                                                                 gTTAGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGCACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGt  >  1:452245/1‑140 (MQ=255)                                                                                                                                    aGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGa             >  2:1138767/1‑126 (MQ=255)                                                                                                                                    aGCCCGCCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGa             <  1:1138767/126‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TCATATCATCCCGCGAACTCAGGCTACGCTCATAGCGCAACCCAGCCTGAGTTTGCTTGATGGTTTTTCGCGTGTCGTACTGTTCTGCACGAGGCGCTTGTTGTGGATTAGCCTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGT  >  NC_000913/1522180‑1522447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑