Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,169	A→G	A185A (GCA→GCG)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,169	0	A	G	100.0%	259.8 / NA	71	A185A (GCA→GCG)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/40);  total (31/40)

CGAATATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCACCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGAAAG  >  NC_000913/132034‑132302                                                                                                                                        |                                                                                                                                      cGAATATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCTAAACTAACACCGATGACCTTTCTCCGGCGCCg                                                                                                                                    <  1:652816/139‑1 (MQ=255) cGAATATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCTAAACTAACACCGATGACCTTTCTCCGGCGCCg                                                                                                                                    >  2:652816/1‑139 (MQ=255)   aaTATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGAt                                                                                                                                 >  2:265548/1‑140 (MQ=255)   aaTATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGAt                                                                                                                                 <  2:876791/140‑1 (MQ=255)       tGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGt                                                                                                                             >  2:786998/1‑140 (MQ=255)         cGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGGCCTTTCTCCGGCGCCGGATGCGTgg                                                                                                                           >  2:461033/1‑140 (MQ=255)          gAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGt                                                                                                                          <  2:191047/140‑1 (MQ=255)          gAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGt                                                                                                                          <  1:380682/140‑1 (MQ=255)              cAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACg                                                                                                                      >  2:54509/1‑140 (MQ=255)               aGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGc                                                                                                                     <  1:786998/140‑1 (MQ=255)               aGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGc                                                                                                                     <  1:375013/140‑1 (MQ=255)                 gTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGc                                                                                                                     <  1:1149506/138‑1 (MQ=255)                 gTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGc                                                                                                                     >  2:1149506/1‑138 (MQ=255)                      gCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGata                                                                                                              <  2:863818/140‑1 (MQ=255)                      gCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGata                                                                                                              >  2:1150479/1‑140 (MQ=255)                          tCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATccc                                                                                                          <  2:1135487/140‑1 (MQ=255)                          tCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATccc                                                                                                          >  1:49151/1‑140 (MQ=255)                           ccTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCa                                                                                                         >  2:4546/1‑140 (MQ=255)                           ccTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCa                                                                                                         <  1:609601/140‑1 (MQ=255)                               ggCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGc                                                                                                                     <  1:872465/124‑1 (MQ=255)                               ggCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGc                                                                                                                     >  2:872465/1‑124 (MQ=255)                                gCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCa                                                                                                    <  2:179771/140‑1 (MQ=255)                                 cGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAc                                                                                                   <  1:1098143/140‑1 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCgg                                                                                                                 <  2:1086838/123‑1 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCgg                                                                                                                 >  1:1086838/1‑123 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATcc                                                                                                           <  2:357409/129‑1 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATcc                                                                                                           >  1:357409/1‑129 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                                >  2:254059/1‑140 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                                >  1:318694/1‑140 (MQ=255)                                    aTGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCAcgcg                                                                                                >  1:736132/1‑140 (MQ=255)                                        cGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTgg                                                                                            <  2:1061608/140‑1 (MQ=255)                                                     aaTCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGaaaaa                                                                               <  1:824162/140‑1 (MQ=255)                                                       tCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACg                                                                             <  1:1083894/140‑1 (MQ=255)                                                         gCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGcc                                                                           >  1:808936/1‑140 (MQ=255)                                                         gCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGcc                                                                           <  1:788170/140‑1 (MQ=255)                                                          cccGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGccc                                                                          >  2:650311/1‑140 (MQ=255)                                                                    gctgAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTAt                                                                <  1:1150479/140‑1 (MQ=255)                                                                         aaaaCTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGaa                                                                     <  1:1091801/130‑1 (MQ=255)                                                                         aaaaCTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGaa                                                                     >  2:1091801/1‑130 (MQ=255)                                                                         aaaaCTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGc                                                           <  1:599941/140‑1 (MQ=255)                                                                              tGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGAc                                                      <  2:808936/140‑1 (MQ=255)                                                                              tGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGAc                                                      <  1:254059/140‑1 (MQ=255)                                                                                   gTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGcc                                                 <  1:4546/140‑1 (MQ=255)                                                                                      aCTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                              <  2:318694/140‑1 (MQ=255)                                                                                      aCTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                              <  2:49151/140‑1 (MQ=255)                                                                                      aCTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                              <  2:631439/140‑1 (MQ=255)                                                                                            ttCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                              <  2:861498/134‑1 (MQ=255)                                                                                            ttCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTgg                                              >  1:861498/1‑134 (MQ=255)                                                                                            ttCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGtgttgt                                        >  2:410712/1‑140 (MQ=255)                                                                                               aaaGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTgg                                     >  1:33120/1‑140 (MQ=255)                                                                                                    cACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGa                                >  1:936416/1‑140 (MQ=255)                                                                                                    cACTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGa                                >  1:199794/1‑140 (MQ=255)                                                                                                     aCTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGt                                    >  2:1013295/1‑135 (MQ=255)                                                                                                     aCTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGt                                    <  1:1013295/135‑1 (MQ=255)                                                                                                      cTGGCGAAACTAACACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATc                              >  2:108964/1‑140 (MQ=255)                                                                                                                 aaCACCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGa                   <  1:410712/140‑1 (MQ=255)                                                                                                                   cacCGATGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAg                 <  1:293307/140‑1 (MQ=255)                                                                                                                        aTGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTg            >  1:542708/1‑140 (MQ=255)                                                                                                                        aTGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTg            >  2:704502/1‑140 (MQ=255)                                                                                                                        aTGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTg            <  2:736132/140‑1 (MQ=255)                                                                                                                         tGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAGGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCaa                        >  2:1018879/1‑127 (MQ=255)                                                                                                                         tGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCaa                        <  1:1018879/127‑1 (MQ=255)                                                                                                                         tGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTg            <  2:178308/139‑1 (MQ=255)                                                                                                                         tGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTg            >  1:178308/1‑139 (MQ=255)                                                                                                                         tGACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGc           >  1:186057/1‑140 (MQ=255)                                                                                                                          gACCTTTCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCa          <  2:186057/140‑1 (MQ=255)                                                                                                                              tttCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAg      <  2:936416/140‑1 (MQ=255)                                                                                                                              tttCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAg      <  2:564629/140‑1 (MQ=255)                                                                                                                              tttCTCCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAg      <  2:33120/140‑1 (MQ=255)                                                                                                                                tctcCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGaa    >  2:1025555/1‑140 (MQ=255)                                                                                                                                tctcCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGaa    <  1:54509/140‑1 (MQ=255)                                                                                                                                  tcCGGCGCCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGAAAg  >  2:143907/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CGAATATGCGAAGCAGGTTATGCAGTCCTGGGCGGATGCCGAATGGTTCCTGAATCGCCCGGCGCTGGCTGAAAAACTGACCGTTACTGTCTTCAAAGTCACTGGCGAAACTAACACCGATGACCTTTCTCCGGCACCGGATGCGTGGTCACGCCCGGATATCCCACTGCACGCGCTGGCGATGCTGAAAAACGCCCGTGAAGGTATTGAGCCAGACCAGCCTGGTGTTGTTGGTCCGATCAAGCAAATCGAAGCTCTGCAACAGAAAG  >  NC_000913/132034‑132302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑