Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,762,529	A→G	V494A (GTC→GCC)	sufB ←	Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,762,529	0	A	G	100.0%	145.4 / NA	41	V494A (GTC→GCC)	sufB	Fe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/14);  total (27/14)

TTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGC  >  NC_000913/1762394‑1762663                                                                                                                                        |                                                                                                                                       ttGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTTAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCt                                                                                                                                    >  2:546755/1‑140 (MQ=255) ttGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTTAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCt                                                                                                                                    <  1:546755/140‑1 (MQ=255)   gCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCtgt                                                                                                                                  <  1:1156768/140‑1 (MQ=255)    cccGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCtgtg                                                                                                                                 >  2:340735/1‑140 (MQ=255)     ccGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCt                                                                                                                                    <  2:353960/136‑1 (MQ=255)     ccGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCt                                                                                                                                    >  1:353960/1‑136 (MQ=255)       gAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTc                                                                                                                              <  1:116965/140‑1 (MQ=255)        aaCCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCa                                                                                                                             >  1:1063212/1‑140 (MQ=255)                   cccATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGc                                                                                                                  >  2:638285/1‑140 (MQ=255)                       tAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCgagg                                                                                                              >  2:821378/1‑140 (MQ=255)                       tAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCgagg                                                                                                              >  2:712213/1‑140 (MQ=255)                       tAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCgagg                                                                                                              >  1:911346/1‑140 (MQ=255)                           ggCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCg                                                                                                                 >  2:887411/1‑133 (MQ=255)                           ggCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCg                                                                                                                 <  1:887411/133‑1 (MQ=255)                                      cGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCa                                                                                               >  1:986791/1‑140 (MQ=255)                                                     cGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAAc                                                                                >  2:293392/1‑140 (MQ=255)                                                       aGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACgg                                                                              >  2:610231/1‑140 (MQ=255)                                                              aTCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTcc                                                                       <  2:1063212/140‑1 (MQ=255)                                                              aTCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTcc                                                                       <  1:712213/140‑1 (MQ=255)                                                                   cgcAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGaa                                                                  >  1:205146/1‑140 (MQ=255)                                                                                  tCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGaa                                                   >  1:1127167/1‑140 (MQ=255)                                                                                       cACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGt                                              >  1:1163478/1‑140 (MQ=255)                                                                                          gCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTaa                                           <  1:290686/140‑1 (MQ=255)                                                                                              aCGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTaa                                           <  2:454179/136‑1 (MQ=255)                                                                                              aCGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTaa                                           >  1:454179/1‑136 (MQ=255)                                                                                               cGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCAGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAatc                                      >  1:636240/1‑140 (MQ=255)                                                                                                        cTTTAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATg                             >  1:1094771/1‑140 (MQ=255)                                                                                                          ttAATACTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGc                           <  2:1127167/140‑1 (MQ=255)                                                                                                            aaTACTTAACATGTTTATTCCTTATCCAGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGt                         <  2:636240/140‑1 (MQ=255)                                                                                                                cTTAACATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGtcttc                     <  2:357852/140‑1 (MQ=255)                                                                                                                   aaCATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGc                           <  1:1050098/131‑1 (MQ=255)                                                                                                                   aaCATGTTTATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGc                           >  2:1050098/1‑131 (MQ=255)                                                                                                                         tttATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGAt            >  2:101956/1‑140 (MQ=255)                                                                                                                          ttATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATc           >  1:391460/1‑140 (MQ=255)                                                                                                                           tATTCCTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATcc          >  1:10873/1‑140 (MQ=255)                                                                                                                               ccTTATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCcgcg      >  1:262583/1‑140 (MQ=255)                                                                                                                                  tATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTg   >  2:1049390/1‑140 (MQ=255)                                                                                                                                  tATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTg   <  1:821378/140‑1 (MQ=255)                                                                                                                                  tATCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTg   >  1:725254/1‑140 (MQ=255)                                                                                                                                   aTCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTgc  >  1:343531/1‑140 (MQ=255)                                                                                                                                   aTCCGGCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTgc  >  1:636503/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TTGCCCGAACCGTTTGGCCCCATAATGGCGTGAACTTCGCCGGGATGAACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGC  >  NC_000913/1762394‑1762663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑