Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,840,159	T→C	R231R (CGT→CGC)	ynjE →	molybdopterin synthase sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,840,159	0	T	C	100.0%	201.7 / NA	55	R231R (CGT→CGC)	ynjE	molybdopterin synthase sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/24);  total (31/24)

GGCGCTGACTACATCGATACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGTGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAG  >  NC_000913/1840028‑1840292                                                                                                                                    |                                                                                                                                      ggCGCTGACTACATCGATACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGtata                                                                                                                               >  2:1020798/1‑140 (MQ=255) ggCGCTGACTACATCGATACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGtata                                                                                                                               >  1:979184/1‑140 (MQ=255) ggCGCTGACTACATCGATACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGtata                                                                                                                               >  1:94000/1‑140 (MQ=255) ggCGCTGACTACATCGATACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGtata                                                                                                                               >  1:69047/1‑140 (MQ=255)              tCGATACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCgt                                                                                                                  <  2:800804/140‑1 (MQ=255)                  tACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTgg                                                                                                              >  2:524001/1‑140 (MQ=255)                  tACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTgg                                                                                                              >  2:524918/1‑140 (MQ=255)                  tACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTgg                                                                                                              >  1:84713/1‑140 (MQ=255)                  tACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTgg                                                                                                              >  2:349010/1‑140 (MQ=255)                   aCCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgc                                                                                                             <  1:349010/140‑1 (MQ=255)                    ccAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgcg                                                                                                            <  2:94000/140‑1 (MQ=255)                    ccAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgcg                                                                                                            <  2:111181/140‑1 (MQ=255)                    ccAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgcg                                                                                                            >  1:948215/1‑140 (MQ=255)                    ccAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgcg                                                                                                            <  1:524918/140‑1 (MQ=255)                    ccAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgcg                                                                                                            >  2:325732/1‑140 (MQ=255)                    ccAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgcg                                                                                                            <  1:524001/140‑1 (MQ=255)                    ccAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGgcg                                                                                                            <  2:283312/140‑1 (MQ=255)                       aCGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAg                                                                                                         >  1:249112/1‑140 (MQ=255)                          aaGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAtt                                                                                                      >  1:492310/1‑140 (MQ=255)                          aaGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAtt                                                                                                      <  2:69047/140‑1 (MQ=255)                          aaGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAtt                                                                                                      <  2:452065/140‑1 (MQ=255)                            gTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGNTGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttat                                                                                                    >  1:212930/1‑140 (MQ=255)                            gTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttat                                                                                                    >  2:1117513/1‑140 (MQ=255)                            gTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGACAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttat                                                                                                    >  2:514021/1‑140 (MQ=255)                               gAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgct                                                                                                 <  2:84713/140‑1 (MQ=255)                                    tGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatg                                                                                            >  2:647294/1‑140 (MQ=255)                                    tGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatg                                                                                            <  2:492310/140‑1 (MQ=255)                                      aaCCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatgct                                                                                          <  1:647294/140‑1 (MQ=255)                                        ccGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTgg                                                                                        <  1:227713/140‑1 (MQ=255)                                         cGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatgct                                                                                          <  1:1102111/137‑1 (MQ=255)                                         cGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatgct                                                                                          >  2:1102111/1‑137 (MQ=255)                                           cTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatg                                                                                            >  2:577987/1‑133 (MQ=255)                                           cTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatg                                                                                            >  2:125093/1‑133 (MQ=255)                                           cTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatg                                                                                            <  1:577987/133‑1 (MQ=255)                                           cTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGAttatgctttatg                                                                                            <  1:125093/133‑1 (MQ=255)                                               ggAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGaaa                                                                                 <  1:1117513/140‑1 (MQ=255)                                                             gatgaACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCctgct                                                                   >  2:249296/1‑140 (MQ=255)                                                                   caacTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATgg                                                             <  2:341084/140‑1 (MQ=255)                                                                      cTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATATGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATggcgg                                                          <  2:979184/140‑1 (MQ=255)                                                                              gATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCaaa                                                  >  2:207253/1‑140 (MQ=255)                                                                                tGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAAcc                                                >  1:137534/1‑140 (MQ=255)                                                                                    ggCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGt                                            <  1:325732/140‑1 (MQ=255)                                                                                          acacGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGAcg                                      >  1:39218/1‑140 (MQ=255)                                                                                                cATTCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGAc                                <  1:1020798/140‑1 (MQ=255)                                                                                                   tCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCggg                                  <  2:1028366/135‑1 (MQ=255)                                                                                                   tCGCCATGACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCggg                                  >  1:1028366/1‑135 (MQ=255)                                                                                                          gACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTg                          <  1:972473/136‑1 (MQ=255)                                                                                                          gACACCACGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTg                          >  2:972473/1‑136 (MQ=255)                                                                                                            caccacGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAgc                    >  1:852543/1‑140 (MQ=255)                                                                                                               cacGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGcgcg                 >  2:133143/1‑140 (MQ=255)                                                                                                                 cGGTCATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGa               >  2:453943/1‑140 (MQ=255)                                                                                                                     cATTCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGAGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGc           >  1:375214/1‑140 (MQ=255)                                                                                                                       ttCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCa         >  1:75590/1‑140 (MQ=255)                                                                                                                        tCTGTATGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCAc        <  2:75590/140‑1 (MQ=255)                                                                                                                            tATGGGCGCGAAGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGaa    >  2:270423/1‑140 (MQ=255)                                                                                                                              tGGGCGCGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAg  >  2:528360/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      GGCGCTGACTACATCGATACCAACGAAGTGGAAAGTGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGTGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAG  >  NC_000913/1840028‑1840292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑