Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,843,243	T→C	G291G (GGT→GGC)	gdhA →	glutamate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,843,243	0	T	C	100.0%	192.6 / NA	53	G291G (GGT→GGC)	gdhA	glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

TACGCTATCGAAAAAGCGATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTA  >  NC_000913/1843106‑1843376                                                                                                                                          |                                                                                                                                      tACGCTATCGAAAAAGCGATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCg                                                                                                                                     >  1:1110078/1‑140 (MQ=255)      tATCGAAAAAGCGATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTgg                                                                                                                                >  1:606331/1‑140 (MQ=255)                  gATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGcc                                                                                                                     >  2:492831/1‑139 (MQ=255)                  gATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGcc                                                                                                                     <  1:492831/139‑1 (MQ=255)                             gTGCTCGTGTAATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTtggt                                                                                                         <  1:617128/140‑1 (MQ=255)                              tGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTtggtc                                                                                                        >  2:522800/1‑140 (MQ=255)                                cTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTtggtctg                                                                                                      >  2:592552/1‑140 (MQ=255)                                 tCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTtggtctgg                                                                                                     >  2:649574/1‑140 (MQ=255)                                             tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTtggtctggt                                                                                                    <  1:233771/129‑1 (MQ=255)                                             tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTtggtctggt                                                                                                    >  2:233771/1‑129 (MQ=255)                                             tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAg                                                                                         >  2:95786/1‑140 (MQ=255)                                             tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAg                                                                                         >  2:903552/1‑140 (MQ=255)                                             tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAg                                                                                         >  2:450841/1‑140 (MQ=255)                                             tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAg                                                                                         <  2:32070/140‑1 (MQ=255)                                             tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAg                                                                                         >  1:130635/1‑140 (MQ=255)                                               cGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGc                                                                                       >  1:913321/1‑140 (MQ=255)                                                gTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGcc                                                                                      <  1:738176/140‑1 (MQ=255)                                                gTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGcc                                                                                      <  1:752106/140‑1 (MQ=255)                                                  cAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGa                                                                                           <  1:528557/133‑1 (MQ=255)                                                  cAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGa                                                                                           >  2:528557/1‑133 (MQ=255)                                                    gACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACa                                                                                  >  1:537521/1‑140 (MQ=255)                                                       tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGcc                                                                               <  2:537521/140‑1 (MQ=255)                                                       tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGcc                                                                               <  2:22334/140‑1 (MQ=255)                                                       tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGcc                                                                               <  1:522800/140‑1 (MQ=255)                                                       tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGcc                                                                               <  1:175695/140‑1 (MQ=255)                                                         cAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGGTTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCgg                                                                             >  2:765247/1‑139 (MQ=255)                                                          aGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTg                                                                            >  1:276457/1‑140 (MQ=255)                                                               cACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGtctc                                                                       >  1:372460/1‑140 (MQ=255)                                                               cACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGtctc                                                                       <  2:130635/140‑1 (MQ=255)                                                                 cTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTa                                                                     <  1:649574/140‑1 (MQ=255)                                                                 cTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTa                                                                     >  2:733137/1‑140 (MQ=255)                                                                      gTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGt                                                                <  1:903552/140‑1 (MQ=255)                                                                      gTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGt                                                                <  1:733137/140‑1 (MQ=255)                                                                          atgaAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGat                                                            <  1:592552/140‑1 (MQ=255)                                                                           tgaAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGata                                                           <  2:606331/140‑1 (MQ=255)                                                                              aaGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCg                                                        >  2:501464/1‑140 (MQ=255)                                                                              aaGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCg                                                        >  2:570084/1‑140 (MQ=255)                                                                              aaGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCg                                                        >  1:593359/1‑140 (MQ=255)                                                                              aaGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCg                                                        >  2:883452/1‑140 (MQ=255)                                                                                gCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGcc                                                      <  1:96944/140‑1 (MQ=255)                                                                                 cGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTa                                                                     >  2:120514/1‑124 (MQ=255)                                                                                 cGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTa                                                                     <  1:120514/124‑1 (MQ=255)                                                                                 cGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGccc                                                     >  2:1158396/1‑140 (MQ=255)                                                                                          gAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTgcg                                            <  1:1158396/140‑1 (MQ=255)                                                                                          gAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTgcg                                            <  1:570084/140‑1 (MQ=255)                                                                                                     tGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAAt                                 <  1:95786/140‑1 (MQ=255)                                                                                                        cACGTCTTATCGAAATCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGaa                              >  2:898269/1‑140 (MQ=255)                                                                                                                     aaTCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGAcg                 >  1:624663/1‑140 (MQ=255)                                                                                                                     aaTCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGAcg                 >  2:775549/1‑140 (MQ=255)                                                                                                                      aTCAAAGCCAGCCGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGAcgc                >  1:444721/1‑140 (MQ=255)                                                                                                                                 ccGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGc     >  2:1105904/1‑140 (MQ=255)                                                                                                                                 ccGCGATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGc     <  1:1105904/140‑1 (MQ=255)                                                                                                                                    cgATGGCCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTa  <  1:883452/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TACGCTATCGAAAAAGCGATGGAATTTGGTGCTCGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTA  >  NC_000913/1843106‑1843376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑