Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,861,665	T→C	intergenic (‑333/+37)	ydjL ← / ← yeaC	putative zinc‑binding dehydrogenase YdjL/conserved protein YeaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,665	0	T	C	100.0%	180.1 / NA	49	intergenic (‑333/+37)	ydjL/yeaC	putative zinc‑binding dehydrogenase YdjL/conserved protein YeaC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

ATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCA  >  NC_000913/1861528‑1861799                                                                                                                                          |                                                                                                                                       aTTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTAccc                                                                                                                                      >  1:107775/1‑140 (MQ=255)        gCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCAt                                                                                                                               >  2:251496/1‑140 (MQ=255)         ccAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATa                                                                                                                              >  2:476708/1‑140 (MQ=255)             aTAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCg                                                                                                                          >  1:416520/1‑140 (MQ=255)                    tGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCaaa                                                                                                                   >  1:906681/1‑140 (MQ=255)                       gaCTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAg                                                                                                                <  1:985309/140‑1 (MQ=255)                           tAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGa                                                                                                            >  1:135766/1‑140 (MQ=255)                             aGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATg                                                                                                          <  1:251496/140‑1 (MQ=255)                                tCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAAc                                                                                                       >  1:1039644/1‑140 (MQ=255)                                  aGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     >  1:626836/1‑140 (MQ=255)                                  aGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     <  1:476708/140‑1 (MQ=255)                                  aGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     <  2:1088938/140‑1 (MQ=255)                                  aGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     <  2:229041/140‑1 (MQ=255)                                  aGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     <  2:626836/140‑1 (MQ=255)                                  aGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     <  2:67866/140‑1 (MQ=255)                                   ggAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCac                                                                                                    <  1:790169/140‑1 (MQ=255)                                       aaGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     <  1:995785/135‑1 (MQ=255)                                       aaGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     >  2:995785/1‑135 (MQ=255)                                          tCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCActtactta                                                                                             <  1:42208/140‑1 (MQ=255)                                            aTTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     <  1:417878/130‑1 (MQ=255)                                            aTTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCa                                                                                                     >  2:417878/1‑130 (MQ=255)                                            aTTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTaaa                                                                                           <  2:416520/140‑1 (MQ=255)                                              tCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACa                                                                                         <  1:1094463/140‑1 (MQ=255)                                              tCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACa                                                                                         <  2:107775/140‑1 (MQ=255)                                                        aCGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAgg                                                                               <  1:305657/140‑1 (MQ=255)                                                               ggCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCt                                                                        >  2:781048/1‑140 (MQ=255)                                                                 cAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTga                                                                      >  1:33680/1‑140 (MQ=255)                                                                           gACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGACGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCaaaa                                                            >  2:148933/1‑140 (MQ=255)                                                                               gggACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAAtctt                                                        >  1:412413/1‑140 (MQ=255)                                                                                      aGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTaa                                                 >  1:274004/1‑140 (MQ=255)                                                                                          aGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAActgc                                             >  2:1058203/1‑140 (MQ=255)                                                                                            gTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAActgctg                                           >  1:1140594/1‑140 (MQ=255)                                                                                                tGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTg                                       <  2:1140594/140‑1 (MQ=255)                                                                                                 ggCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGc                                      <  2:135766/140‑1 (MQ=255)                                                                                                 ggCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGc                                      >  1:784223/1‑140 (MQ=255)                                                                                                  gCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGct                                     <  1:148933/140‑1 (MQ=255)                                                                                                             gagaTGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGATGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCa                          <  2:485297/140‑1 (MQ=255)                                                                                                              agaTGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCAt                         <  1:781048/140‑1 (MQ=255)                                                                                                               gaTGAGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATc                        >  2:1066063/1‑140 (MQ=255)                                                                                                                   aGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGAc                    >  2:1117185/1‑140 (MQ=255)                                                                                                                   aGTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGAc                    >  1:349748/1‑140 (MQ=255)                                                                                                                   aGTCGTGGGGCCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGAc                    >  2:502591/1‑140 (MQ=255)                                                                                                                    gTCGTGGGGTCATTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGAcc                   >  1:967623/1‑140 (MQ=255)                                                                                                                               aTTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCaa        <  2:412413/140‑1 (MQ=255)                                                                                                                               aTTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCaa        <  2:629234/140‑1 (MQ=255)                                                                                                                               aTTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCaa        >  1:836553/1‑140 (MQ=255)                                                                                                                               aTTGTTGGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGAAACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCaa        >  1:321386/1‑140 (MQ=255)                                                                                                                                 tgttgGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAAtt      <  2:274004/140‑1 (MQ=255)                                                                                                                                    tgGCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTc   <  1:738261/140‑1 (MQ=255)                                                                                                                                     ggCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCa  >  1:906943/1‑140 (MQ=255)                                                                                                                                     ggCTACCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCa  >  2:220710/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTGAACGGGACGGCAAGAGATCGGACTGGGACTTAGCCAGGTCATGGCGAGATAATTGAGATGAGTCGTGGGGTCATTGTTGGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCA  >  NC_000913/1861528‑1861799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑