BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,195	T→C	D93G (GAC→GGC)	mipA ←	scaffolding protein that interacts with murein polymerase and murein hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,195	0	T	C	100.0%	382.0 / NA	102	D93G (GAC→GGC)	mipA	scaffolding protein that interacts with murein polymerase and murein hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/49); total (53/49)

Only 100 of 102 total aligned reads displayed.

TGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTAC  >  NC_000913/1866058‑1866328

tGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCt                                                                                                                                     >  1:1055686/1‑140 (MQ=255)

tGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCt                                                                                                                                     >  2:233991/1‑140 (MQ=255)

tGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCt                                                                                                                                     >  1:599976/1‑140 (MQ=255)

 gCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCtt                                                                                                                                    >  1:59448/1‑140 (MQ=255)

 gCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCtt                                                                                                                                    >  1:773725/1‑140 (MQ=255)

  ccGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCttt                                                                                                                                   >  2:132284/1‑140 (MQ=255)

             aTCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGt                                                                                                                        >  1:749484/1‑140 (MQ=255)

                   ggTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAgcg                                                                                                                  >  2:348629/1‑140 (MQ=255)

                     tATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAgcggc                                                                                                                >  1:214049/1‑140 (MQ=255)

                     tATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAgcggc                                                                                                                >  2:903618/1‑140 (MQ=255)

                     tATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAgcggc                                                                                                                <  2:749484/140‑1 (MQ=255)

                      aTCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAgcggcg                                                                                                               <  2:555497/140‑1 (MQ=255)

                       tCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGa                                                                                                              >  1:274747/1‑140 (MQ=255)

                        cGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGAc                                                                                                             >  1:548080/1‑140 (MQ=255)

                              ccagGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTaa                                                                                                       >  2:303929/1‑140 (MQ=255)

                                       tACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGt                                                                                                         <  2:1014025/129‑1 (MQ=255)

                                       tACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGt                                                                                                         >  1:1014025/1‑129 (MQ=255)

                                       tACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAAtt                                                                                              >  2:929795/1‑140 (MQ=255)

                                        aCGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTg                                                                                             >  2:159090/1‑140 (MQ=255)

                                            aGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAg                                                                                         >  1:69296/1‑140 (MQ=255)

                                             ggTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGt                                                                                        >  1:1090541/1‑140 (MQ=255)

                                              gTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGtt                                                                                       >  2:607730/1‑140 (MQ=255)

                                                aaCCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTa                                                                                     >  2:1007131/1‑140 (MQ=255)

                                                aaCCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTa                                                                                     >  2:1043896/1‑140 (MQ=255)

                                                       cTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTg                                                                              <  1:159090/140‑1 (MQ=255)

                                                        tGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGc                                                                             >  2:810190/1‑140 (MQ=255)

                                                          ggTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGt                                                                           <  2:59448/140‑1 (MQ=255)

                                                          ggTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGt                                                                           <  1:929795/140‑1 (MQ=255)

                                                          ggTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGt                                                                           >  2:417184/1‑140 (MQ=255)

                                                           gTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTc                                                                          <  1:208376/140‑1 (MQ=255)

                                                            tAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCACCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCa                                                                         <  2:821247/140‑1 (MQ=255)

                                                               aGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCg                                                                            >  1:1133765/1‑134 (MQ=255)

                                                               aGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCg                                                                            <  2:1133765/134‑1 (MQ=255)

                                                               aGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTc                                                                      >  2:1087826/1‑140 (MQ=255)

                                                               aGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTc                                                                      <  2:780627/140‑1 (MQ=255)

                                                               aGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTc                                                                      >  1:770927/1‑140 (MQ=255)

                                                               aGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTc                                                                      >  2:1150764/1‑140 (MQ=255)

                                                                 tGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCca                                                                    <  2:548080/140‑1 (MQ=255)

                                                                 tGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCca                                                                    <  1:132284/140‑1 (MQ=255)

                                                                  gAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCcac                                                                   <  1:1087826/140‑1 (MQ=255)

                                                                    gCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAg                                                                 >  2:404659/1‑140 (MQ=255)

                                                                    gCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAg                                                                 >  1:161383/1‑140 (MQ=255)

                                                                    gCATAAGACAGACCAGCCATCATGATGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAg                                                                 <  2:697372/140‑1 (MQ=255)

                                                                     cATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAgg                                                                <  1:1150764/140‑1 (MQ=255)

                                                                       tAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGgta                                                              <  1:348629/140‑1 (MQ=255)

                                                                             agacCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAacc                                                        <  1:812425/140‑1 (MQ=255)

                                                                             agacCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAacc                                                        <  2:773725/140‑1 (MQ=255)

                                                                              gacCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAacca                                                       <  2:256695/140‑1 (MQ=255)

                                                                              gacCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAacca                                                       <  2:250953/140‑1 (MQ=255)

                                                                               acCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAaccac                                                      >  2:528133/1‑140 (MQ=255)

                                                                               acCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAaccac                                                      >  1:5126/1‑140 (MQ=255)

                                                                                ccAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAaccacc                                                     >  2:841267/1‑140 (MQ=255)

                                                                                    ccATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAacca                                                       <  1:601755/134‑1 (MQ=255)

                                                                                    ccATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAacca                                                       >  2:601755/1‑134 (MQ=255)

                                                                                         atGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGcc                                            >  2:772034/1‑140 (MQ=255)

                                                                                         atGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGcc                                            >  1:419800/1‑140 (MQ=255)

                                                                                           ggTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          <  1:772034/140‑1 (MQ=255)

                                                                                           ggTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          <  1:746050/140‑1 (MQ=255)

                                                                                           ggTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          <  1:175292/140‑1 (MQ=255)

                                                                                           ggTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          <  2:645309/140‑1 (MQ=255)

                                                                                                tctTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAAc                                     <  1:1007131/140‑1 (MQ=255)

                                                                                                 ctTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          >  1:515805/1‑134 (MQ=255)

                                                                                                 ctTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          <  2:515805/134‑1 (MQ=255)

                                                                                                  ttACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          <  2:740001/133‑1 (MQ=255)

                                                                                                  ttACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCAc                                          >  1:740001/1‑133 (MQ=255)

                                                                                                  ttACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCa                                   >  2:928679/1‑140 (MQ=255)

                                                                                                  ttACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCa                                   >  1:734872/1‑140 (MQ=255)

                                                                                                   tACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAg                                  <  2:1061927/140‑1 (MQ=255)

                                                                                                   tACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAg                                  <  1:404659/140‑1 (MQ=255)

                                                                                                   tACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAg                                  <  1:928679/140‑1 (MQ=255)

                                                                                                    aCGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAaccaccacc                                                  <  1:333584/123‑1 (MQ=255)

                                                                                                    aCGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAaccaccacc                                                  >  2:333584/1‑123 (MQ=255)

                                                                                                    aCGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGa                                 >  1:697497/1‑140 (MQ=255)

                                                                                                        tCATCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGtt                             <  2:875836/140‑1 (MQ=255)

                                                                                                           tCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATc                          >  2:1147184/1‑140 (MQ=255)

                                                                                                           tCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATc                          >  2:568581/1‑140 (MQ=255)

                                                                                                           tCCAGGTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATc                          <  1:841267/140‑1 (MQ=255)

                                                                                                                gTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATc                          >  2:541540/1‑135 (MQ=255)

                                                                                                                gTGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATc                          <  1:541540/135‑1 (MQ=255)

                                                                                                                 tGACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTc                    <  2:69296/140‑1 (MQ=255)

                                                                                                                  gACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCa                   <  1:1043896/140‑1 (MQ=255)

                                                                                                                  gACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCa                   <  1:233991/140‑1 (MQ=255)

                                                                                                                  gACGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCa                   >  1:1119510/1‑140 (MQ=255)

                                                                                                                   aCGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCAt                  <  1:568581/140‑1 (MQ=255)

                                                                                                                    cGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATa                 <  2:1055686/140‑1 (MQ=255)

                                                                                                                    cGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATa                 >  1:1030790/1‑140 (MQ=255)

                                                                                                                    cGCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATa                 >  1:186663/1‑140 (MQ=255)

                                                                                                                     gCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAg                >  1:437354/1‑140 (MQ=255)

                                                                                                                     gCATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAg                >  2:562605/1‑140 (MQ=255)

                                                                                                                      cATTTGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGt               >  2:308890/1‑140 (MQ=255)

                                                                                                                        tttGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTg             <  2:734872/140‑1 (MQ=255)

                                                                                                                        ccaggTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTg             <  2:495496/137‑1 (MQ=255)

                                                                                                                          tGGTGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGAt           <  2:274747/140‑1 (MQ=255)

                                                                                                                             tGATCGCCACTGCCTTTCGCTTTGAATTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTAc        <  2:1030790/140‑1 (MQ=255)

                                                                                                                             tGATCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTAc        <  1:417184/140‑1 (MQ=255)

                                                                                                                               aTCGCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCg      >  1:279281/1‑140 (MQ=255)

                                                                                                                                  gCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTa   <  1:957159/140‑1 (MQ=255)

                                                                                                                                  gCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTa   <  1:506033/140‑1 (MQ=255)

                                                                                                                                  gCCACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTa   <  1:600378/140‑1 (MQ=255)

                                                                                                                                   ccACTGCCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTAc  <  2:186663/140‑1 (MQ=255)

TGCCGTTGCTGTTATCCAGGGTATCGCCAGCCAGGGTGGTACGCAGGTAACCGTACTGGGTAAAGTGAGCATAAGACAGACCAGCCATCATGGTGCTCTTACGGTCATCCAGGTGACGCATTTGGTGATCGCCACTGTCTTTCGCTTTGAAGTAAAGCGGCGACCAGTAAGCGGTAATTGAAAGTTTATCCGTTGCGTCATTCCACAGGTAGTAACCACCACCTAAGCCACGGAACCAGAAGTTATCGCCTTCATAGTTGATTACCGGTAC  >  NC_000913/1866058‑1866328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`