Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,017,094	T→C	F181L (TTT→CTT)	fliI →	flagellum‑specific ATP synthase FliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,017,094	0	T	C	100.0%	140.0 / NA	39	F181L (TTT→CTT)	fliI	flagellum‑specific ATP synthase FliI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/15);  total (24/15)

ACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGTTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCT  >  NC_000913/2016959‑2017224                                                                                                                                        |                                                                                                                                   acgacgGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGc                                                                                                                                >  1:186143/1‑140 (MQ=255)    acgGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCgg                                                                                                                             >  1:540147/1‑140 (MQ=255)                             cGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGtgctg                                                                                                    >  1:1110765/1‑140 (MQ=255)                              gCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGtgctgc                                                                                                   >  2:989560/1‑140 (MQ=255)                                cATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGctgctg                                                                                                 >  1:341220/1‑140 (MQ=255)                                  tttAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTggg                                                                                               <  2:985726/140‑1 (MQ=255)                                          gTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATgg                                                                                       <  2:186143/140‑1 (MQ=255)                                            tGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCa                                                                                     >  2:473387/1‑140 (MQ=255)                                            tGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTACGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCa                                                                                     >  2:818160/1‑140 (MQ=255)                                                 cGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTa                                                                                <  2:540147/140‑1 (MQ=255)                                                     cacCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACAcc                                                                            <  2:341220/140‑1 (MQ=255)                                                          aTTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCgcgc                                                                       >  1:349544/1‑140 (MQ=255)                                                          aTTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGACACGTTACACCCgcgc                                                                       >  1:362167/1‑140 (MQ=255)                                                             gAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGa                                                                    <  1:473387/140‑1 (MQ=255)                                                             gAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGa                                                                    >  1:238577/1‑140 (MQ=255)                                                             gAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGa                                                                    >  1:1159049/1‑140 (MQ=255)                                                               aCATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATg                                                                  <  2:349544/140‑1 (MQ=255)                                                               aCATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGACACGTTACACCCGCGCCGATg                                                                  <  2:362167/140‑1 (MQ=255)                                                                     gCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTg                                                            >  2:611501/1‑140 (MQ=255)                                                                         gACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGcc                                                                      >  2:8637/1‑126 (MQ=255)                                                                         gACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGcc                                                                      <  1:8637/126‑1 (MQ=255)                                                                              cGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGtt                                                   >  2:176829/1‑140 (MQ=255)                                                                              cGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGtt                                                   >  2:10285/1‑140 (MQ=255)                                                                                 cGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGa                                                <  1:10285/140‑1 (MQ=255)                                                                                   tGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGAtt                                              >  2:701870/1‑140 (MQ=255)                                                                                                tGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGgc                                 >  2:710128/1‑140 (MQ=255)                                                                                                    cTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGAt                                               <  2:321091/122‑1 (MQ=255)                                                                                                    cTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGAt                                               >  1:321091/1‑122 (MQ=255)                                                                                                    cTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGa                             >  1:1024976/1‑140 (MQ=255)                                                                                                     tGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGaa                            >  2:369896/1‑140 (MQ=255)                                                                                                      gCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAg                           >  2:671656/1‑140 (MQ=255)                                                                                                             gTTGGGCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGa                    <  2:1024976/140‑1 (MQ=255)                                                                                                                 ggCGTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGAtttt                >  1:543202/1‑140 (MQ=255)                                                                                                                    gTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTAtt             <  2:238577/140‑1 (MQ=255)                                                                                                                    gTGGGCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTAtt             <  2:1159049/140‑1 (MQ=255)                                                                                                                      gggCAGCGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTga           >  2:527079/1‑140 (MQ=255)                                                                                                                            cGTATGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACAt     >  2:776003/1‑140 (MQ=255)                                                                                                                               aTGGGGCTGCTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCt  <  1:989560/140‑1 (MQ=255)                                                                                                                               aTGGGGCTGCTTGCCGGGTACGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCt  <  1:818160/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   ACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCGTGGGCAGCGTATGGGGCTGTTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCT  >  NC_000913/2016959‑2017224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑