Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,037,637	G→A	C172C (TGC→TGT)	hprS ←	sensory histidine kinase HprS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,037,637	0	G	A	100.0%	166.1 / NA	47	C172C (TGC→TGT)	hprS	sensory histidine kinase HprS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/18);  total (29/18)

AGAGGTTTTAGTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTA  >  NC_000913/2037500‑2037770                                                                                                                                          |                                                                                                                                      agagGTTTTAGTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACa                                                                                                                                     <  2:326349/140‑1 (MQ=255)       tttAGTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTAc                                                                                                                               >  2:180682/1‑140 (MQ=255)             tctctcGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGc                                                                                                                         >  1:577822/1‑140 (MQ=255)               tctcGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGa                                                                                                                       >  1:326523/1‑140 (MQ=255)                 tcGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACa                                                                                                                     >  1:666258/1‑140 (MQ=255)                      aaTGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCa                                                                                                                <  1:334962/140‑1 (MQ=255)                               aCCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAAt                                                                                                       >  1:966310/1‑140 (MQ=255)                                    aaCAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTAt                                                                                                  >  2:118169/1‑140 (MQ=255)                                         gCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAAtt                                                                                             >  1:1008143/1‑140 (MQ=255)                                         gCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAAtt                                                                                             >  1:1002705/1‑140 (MQ=255)                                         gCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAAtt                                                                                             >  2:443832/1‑140 (MQ=255)                                              cGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTtata                                                                                        <  2:666258/140‑1 (MQ=255)                                              cGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTtata                                                                                        <  1:440053/140‑1 (MQ=255)                                              cGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTtata                                                                                        <  2:1096242/140‑1 (MQ=255)                                                        tataATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGc                                                                              <  2:966310/140‑1 (MQ=255)                                                                aGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATg                                                                      >  2:626462/1‑140 (MQ=255)                                                                aGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATg                                                                      >  2:555861/1‑140 (MQ=255)                                                                 gcgcTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGt                                                                     >  1:849167/1‑140 (MQ=255)                                                                  cgcTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTc                                                                    >  1:765940/1‑140 (MQ=255)                                                                  cgcTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTc                                                                    >  2:951080/1‑140 (MQ=255)                                                                  cgcTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTc                                                                    >  2:517229/1‑140 (MQ=255)                                                                  cgcTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTc                                                                    >  2:906991/1‑140 (MQ=255)                                                                          ttACACCACTCAACTTTTTGATCTCTCGTAATCCCATTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGaa                                                            <  2:801494/140‑1 (MQ=255)                                                                                  cTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAAttt                                                    >  2:66071/1‑140 (MQ=255)                                                                                  cTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAAttt                                                    >  1:689284/1‑140 (MQ=255)                                                                                  cTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAAttt                                                    >  2:458418/1‑140 (MQ=255)                                                                                  cTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAAttt                                                    >  2:348347/1‑140 (MQ=255)                                                                                           ttGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGt                                           <  1:590233/140‑1 (MQ=255)                                                                                            tGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTa                                          <  2:849167/140‑1 (MQ=255)                                                                                             gATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTaa                                         <  1:66071/140‑1 (MQ=255)                                                                                                     gTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGt                                           >  2:783895/1‑130 (MQ=255)                                                                                                     gTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGt                                           <  1:783895/130‑1 (MQ=255)                                                                                                     gTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAat                                 >  2:372919/1‑140 (MQ=255)                                                                                                          cccGTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATgg                            <  1:626462/140‑1 (MQ=255)                                                                                                             gTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGa                         >  2:1062831/1‑140 (MQ=255)                                                                                                             gTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGa                         >  1:911675/1‑140 (MQ=255)                                                                                                             gTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGa                         >  1:350787/1‑140 (MQ=255)                                                                                                             gTTCTGATTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGa                         >  2:709283/1‑140 (MQ=255)                                                                                                                   aTTAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTtc                   >  1:431510/1‑140 (MQ=255)                                                                                                                     tAACAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTtcat                 <  1:709283/140‑1 (MQ=255)                                                                                                                        cAGCGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCaa              <  2:689284/140‑1 (MQ=255)                                                                                                                           cGGACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAatat           >  2:360865/1‑140 (MQ=255)                                                                                                                             gACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTa         >  1:1022143/1‑140 (MQ=255)                                                                                                                             gACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTa         <  1:360865/140‑1 (MQ=255)                                                                                                                             gACTTAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTa         <  2:1008143/140‑1 (MQ=255)                                                                                                                                ttAATACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAAtt      <  2:1002705/140‑1 (MQ=255)                                                                                                                                    tACTGAACAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTa  <  2:911675/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AGAGGTTTTAGTTCTCTCGGTAATGCGCTAACCTCAACAGGCTCTCGGCTATCGTTATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTAACAGCGGACTTAATACTGAGCAAAGTACAATGGCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTA  >  NC_000913/2037500‑2037770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑