Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,100,011	T→C	T420A (ACC→GCC)	gnd ←	6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,100,011	0	T	C	100.0%	298.3 / NA	81	T420A (ACC→GCC)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/40);  total (41/40)

TCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCA  >  NC_000913/2099875‑2100141                                                                                                                                         |                                                                                                                                   tCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCgg                                                                                                                                 <  1:1021482/140‑1 (MQ=255) tCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCgg                                                                                                                                 <  2:104370/140‑1 (MQ=255) tCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCgg                                                                                                                                 <  2:35218/140‑1 (MQ=255)           acacacCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATa                                                                                                                       <  1:720934/140‑1 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCgg                                                                                                                                 >  1:688821/1‑126 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCg                                                                                                                            <  1:258724/131‑1 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCg                                                                                                                            >  2:258724/1‑131 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGt                                                                                                                   >  1:83878/1‑140 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGt                                                                                                                   >  2:328980/1‑140 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGCAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGt                                                                                                                   >  2:656765/1‑140 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCA‑GCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGtt                                                                                                                  >  1:881136/1‑140 (MQ=255)               acCTTCTTTATCAATACGCTTATAAGTATGCGCAACAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCgg                                                                                                                                 <  2:688821/126‑1 (MQ=255)                ccTTCTTTATCAATACGCTTAAAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGtt                                                                                                                  <  2:29858/140‑1 (MQ=255)                    cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGtt                                                                                                                  <  2:560176/136‑1 (MQ=255)                    cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGtt                                                                                                                  >  1:560176/1‑136 (MQ=255)                    cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGt                                                                                                              >  2:357281/1‑140 (MQ=255)                    cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGt                                                                                                              <  2:83878/140‑1 (MQ=255)                    cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGt                                                                                                              <  1:357281/140‑1 (MQ=255)                          cAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCa                                                                                                        <  2:882562/140‑1 (MQ=255)                          cAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCa                                                                                                        <  1:142443/140‑1 (MQ=255)                             tACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATaa                                                                                                     >  2:1059378/1‑140 (MQ=255)                             tACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATaa                                                                                                     >  1:75806/1‑140 (MQ=255)                             tACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCACTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATaa                                                                                                     >  1:973202/1‑140 (MQ=255)                              aCGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAg                                                                                                    <  1:328980/140‑1 (MQ=255)                              aCGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAg                                                                                                    >  1:660998/1‑140 (MQ=255)                              aCGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAg                                                                                                    >  2:805349/1‑140 (MQ=255)                               cGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGc                                                                                                   <  1:675365/140‑1 (MQ=255)                                 cTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCaa                                                                                                 <  1:1059378/140‑1 (MQ=255)                                 cTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCaa                                                                                                 <  2:75806/140‑1 (MQ=255)                                  ttATAAGTATGCGCACCAAAATAGTCA‑GCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACg                                                                                               <  2:881136/140‑1 (MQ=255)                                           tGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACg                                                                                       >  2:125922/1‑140 (MQ=255)                                                aCCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAgcg                                                                                  >  1:460559/1‑140 (MQ=255)                                                aCCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAgcg                                                                                  >  2:1133109/1‑140 (MQ=255)                                                   aaaaTAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCct                                                                               >  2:1018045/1‑140 (MQ=255)                                                     aaTAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCctgc                                                                             >  2:563158/1‑140 (MQ=255)                                                            aCGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGt                                                                      >  2:815899/1‑140 (MQ=255)                                                                  tgCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCgg                                                                >  2:1144207/1‑140 (MQ=255)                                                                         aTCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTg                                                         <  1:531636/140‑1 (MQ=255)                                                                          tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGc                                                        <  2:1136841/140‑1 (MQ=255)                                                                            aGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCgg                                                                <  2:621226/130‑1 (MQ=255)                                                                            aGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCgg                                                                >  1:621226/1‑130 (MQ=255)                                                                            aGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCtt                                                      >  1:785088/1‑140 (MQ=255)                                                                              gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGa                                                    >  2:606683/1‑140 (MQ=255)                                                                              gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGa                                                    >  2:494525/1‑140 (MQ=255)                                                                              gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGa                                                    >  2:485202/1‑140 (MQ=255)                                                                              gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGa                                                    >  2:750156/1‑140 (MQ=255)                                                                              gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGACAATTTGCTTGa                                                    >  1:1070653/1‑140 (MQ=255)                                                                                 cGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGt                                                 <  1:606683/140‑1 (MQ=255)                                                                                  gcaggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTa                                                >  2:798041/1‑140 (MQ=255)                                                                                   caggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTAc                                               >  1:434823/1‑140 (MQ=255)                                                                                   caggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTAGTAGTCATCGGCAATTTGCTTGAAGTAc                                               >  2:331001/1‑140 (MQ=255)                                                                                    aggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACg                                              <  2:246804/140‑1 (MQ=255)                                                                                       cagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAg                                           <  1:1144207/140‑1 (MQ=255)                                                                                        agAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGc                                          <  1:815899/140‑1 (MQ=255)                                                                                         gAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGcc                                         >  1:654673/1‑140 (MQ=255)                                                                                           aCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAg                                       <  2:654673/140‑1 (MQ=255)                                                                                            cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGc                                      <  1:1131069/140‑1 (MQ=255)                                                                                              gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    <  1:485202/140‑1 (MQ=255)                                                                                              gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    <  1:798041/140‑1 (MQ=255)                                                                                              gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    <  1:805349/140‑1 (MQ=255)                                                                                              gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTAGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    <  1:331001/140‑1 (MQ=255)                                                                                                agcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACTGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACa                                  <  1:494525/140‑1 (MQ=255)                                                                                                agcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACa                                  >  1:840927/1‑140 (MQ=255)                                                                                                agcaCGGTAGCTGTCGTAATAGGCAACCACTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACa                                  <  2:973202/140‑1 (MQ=255)                                                                                                  caCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    >  1:873273/1‑136 (MQ=255)                                                                                                  caCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    <  2:873273/136‑1 (MQ=255)                                                                                                  caCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAgg                                >  1:662184/1‑140 (MQ=255)                                                                                                          cTGTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGACTACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGACAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGAt                        <  2:1070653/140‑1 (MQ=255)                                                                                                            gTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    <  2:1088518/126‑1 (MQ=255)                                                                                                            gTCGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                    >  1:1088518/1‑126 (MQ=255)                                                                                                              cGTAATAGGCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCtgt                    <  1:750156/140‑1 (MQ=255)                                                                                                                      gCAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTc            >  2:256434/1‑140 (MQ=255)                                                                                                                       cAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAg                            <  2:755365/123‑1 (MQ=255)                                                                                                                       cAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAg                            >  1:755365/1‑123 (MQ=255)                                                                                                                       cAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCg           >  2:627494/1‑140 (MQ=255)                                                                                                                       cAACCGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCg           >  1:121759/1‑140 (MQ=255)                                                                                                                           cGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCAt       <  1:627494/140‑1 (MQ=255)                                                                                                                           cGCTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCAt       <  2:434823/140‑1 (MQ=255)                                                                                                                             cTGCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATaa     >  1:418288/1‑140 (MQ=255)                                                                                                                               gCGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGc   <  1:656765/140‑1 (MQ=255)                                                                                                                                cGGAGAAGGCCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCa  >  1:873716/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCA  >  NC_000913/2099875‑2100141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑