Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,134,136 G→A A506V (GCG→GTG)  wzc ← protein‑tyrosine kinase Wzc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,134,1360GA95.7% 143.0 / NA 47A506V (GCG→GTG) wzcprotein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/17);  minor base C (0/2);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGT  >  NC_000913/2134003‑2134270
                                                                                                                                     |                                                                                                                                      
cACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGAt                                                                                                                                  >  2:310756/1‑140 (MQ=255)
cACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGAt                                                                                                                                  <  2:1126532/140‑1 (MQ=255)
    gccgccAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGt                                                                                                                              >  1:721772/1‑140 (MQ=255)
         cAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGAt                                                                                                                         <  2:601054/140‑1 (MQ=255)
                   cAGACAAAGGTTTTACCAATTGACGGGCTACCCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCCCCAGATCGGTTGGATTCCCCACCGc                                                                                                               <  2:325898/140‑1 (MQ=255)
                            gTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTg                                                                                                      <  1:310756/140‑1 (MQ=255)
                               ttACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGct                                                                                                   <  1:347656/140‑1 (MQ=255)
                                tACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTg                                                                                                      <  2:896701/136‑1 (MQ=255)
                                tACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTg                                                                                                      >  1:896701/1‑136 (MQ=255)
                                      ttGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTta                                                                                            >  2:794330/1‑140 (MQ=255)
                                      ttGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTta                                                                                            >  2:658863/1‑140 (MQ=255)
                                        gACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTtata                                                                                          <  1:1123686/140‑1 (MQ=255)
                                           gggCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCg                                                                                       >  2:736260/1‑140 (MQ=255)
                                                       gTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCttt                                                                           >  1:1084106/1‑140 (MQ=255)
                                                       gTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCttt                                                                           >  1:667788/1‑140 (MQ=255)
                                                       gTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCttt                                                                           >  1:416709/1‑140 (MQ=255)
                                                           tcatcaACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATg                                                                       >  1:599147/1‑140 (MQ=255)
                                                            catcaACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAAt                                                                                 >  2:889294/1‑129 (MQ=255)
                                                            catcaACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAAt                                                                                 <  1:889294/129‑1 (MQ=255)
                                                             atcaACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAAt                                                                                 >  1:1025227/1‑128 (MQ=255)
                                                             atcaACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAAt                                                                                 <  2:1025227/128‑1 (MQ=255)
                                                                 acacATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAAt                                                                                 >  2:428070/1‑124 (MQ=255)
                                                                 acacATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAAt                                                                                 <  1:428070/124‑1 (MQ=255)
                                                                      ttGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCt                                                            <  2:721772/140‑1 (MQ=255)
                                                                         ttCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATc                                                         >  1:648535/1‑140 (MQ=255)
                                                                            tgtgCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCAcg                                                      <  1:658863/140‑1 (MQ=255)
                                                                                   gCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTc                                               >  2:449179/1‑140 (MQ=255)
                                                                                       caccgcgAGGTGCAAACTGGTGCGCAGACTAGGGGTGGCTTCAAGCCCAAGATGGGTTGGATTCCCCTCCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGcc                                           <  2:255848/137‑1 (MQ=255)
                                                                                        atcGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCa                                          >  2:249574/1‑140 (MQ=255)
                                                                                        atcGCGAAGCGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCa                                          >  1:255848/1‑140 (MQ=255)
                                                                                          cgcgAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCAtt                                        >  2:170607/1‑140 (MQ=255)
                                                                                                gTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGa                                    >  2:931587/1‑138 (MQ=255)
                                                                                                gTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGa                                    <  1:931587/138‑1 (MQ=255)
                                                                                                 tGCAAACTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAg                                 >  2:517597/1‑140 (MQ=255)
                                                                                                    aaaCTGGTACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCgg                              <  1:449179/140‑1 (MQ=255)
                                                                                                           tACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGa                                    <  2:900948/127‑1 (MQ=255)
                                                                                                           tACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGa                                    >  1:900948/1‑127 (MQ=255)
                                                                                                           tACGCAGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTg                       >  1:1109245/1‑140 (MQ=255)
                                                                                                                aGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATa                  <  1:153368/140‑1 (MQ=255)
                                                                                                                aGACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATa                  <  1:170607/140‑1 (MQ=255)
                                                                                                                aGACTACGGATGGCTTAAATCCCCAGATCGGTTGGATCCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATa                  <  2:290676/140‑1 (MQ=255)
                                                                                                                 gACTAGGGATGGTTTCAATCACCAGATCGGTTGGATCCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAg                 <  2:1110162/140‑1 (MQ=255)
                                                                                                                 gACTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAg                 <  1:794330/140‑1 (MQ=255)
                                                                                                                  aCTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGa                >  1:290676/1‑140 (MQ=255)
                                                                                                                  aCTACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCAGGATGCTGGCATAGa                >  2:886276/1‑140 (MQ=255)
                                                                                                                    tACGGATGGCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACg              >  2:198861/1‑140 (MQ=255)
                                                                                                                          tggttcaaaTCTCCAGATCGTTTGTTTCCCCCCCCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATa        <  2:667788/133‑1 (MQ=255)
                                                                                                                            gCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATa                  >  1:77606/1‑128 (MQ=255)
                                                                                                                            gCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATa                  <  2:77606/128‑1 (MQ=255)
                                                                                                                            gCTTCAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATAcc      >  2:870617/1‑140 (MQ=255)
                                                                                                                              ttaaaTCCCCATATCGGTTGGATCCCCGACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCgt    <  2:1084106/137‑1 (MQ=255)
                                                                                                                               tCAATCCCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCgtg   <  1:414018/140‑1 (MQ=255)
                                                                                                                                cAATCACCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAAtctgtctc  >  1:150344/1‑133 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGT  >  NC_000913/2134003‑2134270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑