Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,287,586	T→C	pseudogene (1329/2525 nt)	yejO ←	adhesin‑like autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,287,586	0	T	C	100.0%	218.2 / NA	59	pseudogene (1329/2525 nt)	yejO	adhesin‑like autotransporter YejO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

CTGCTGCCGGGCTGGCACCGGTGTCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGTATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGGTGTTTTGGTT  >  NC_000913/2287451‑2287715                                                                                                                                        |                                                                                                                                  ctgctgCCGGGCTGGCACCGGTGTCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATtg                                                                                                                               <  1:646833/140‑1 (MQ=255) ctgctgCCGGGCTGGCACCGGTGTCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATtg                                                                                                                               <  2:571143/140‑1 (MQ=255)      gcCGGGCTGGCACCGGTGTCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTggtggt                                                                                                                          <  1:104126/140‑1 (MQ=255)         gggCTGGCACCGGTGTCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCaa                                                                                                                       >  2:562438/1‑140 (MQ=255)                     gtgtCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGGCATATCGATACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAAtt                                                                                                           <  2:156318/140‑1 (MQ=255)                     gtgtCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAAtt                                                                                                           >  1:708668/1‑140 (MQ=255)                     gtgtCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAAtt                                                                                                           >  2:1096815/1‑140 (MQ=255)                     gtgtCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAAtt                                                                                                           >  2:973295/1‑140 (MQ=255)                         ccGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTcc                                                                                                       >  2:1121546/1‑140 (MQ=255)                         ccGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTcc                                                                                                       >  2:48444/1‑140 (MQ=255)                          cGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCg                                                                                                      <  2:972997/140‑1 (MQ=255)                            tCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCa                                                                                                    <  2:957661/140‑1 (MQ=255)                                   tatTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCg                                                                                             <  1:1096815/140‑1 (MQ=255)                                    atTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGt                                                                                            <  1:317329/140‑1 (MQ=255)                                    atTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGt                                                                                            <  1:973295/140‑1 (MQ=255)                                         gAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACtg                                                                                       <  1:562438/140‑1 (MQ=255)                                            aTCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACtgatg                                                                                    >  2:878653/1‑140 (MQ=255)                                                       ccTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCa                                                                                          >  2:764818/1‑123 (MQ=255)                                                       ccTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCa                                                                                          <  1:764818/123‑1 (MQ=255)                                                       ccTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACt                                                                         <  2:1089074/140‑1 (MQ=255)                                                         tGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTgg                                                                       >  2:978102/1‑140 (MQ=255)                                                         tGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTgg                                                                       <  1:1121546/140‑1 (MQ=255)                                                         tGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTgg                                                                       <  1:644279/140‑1 (MQ=255)                                                           aCCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCt                                                                     >  2:272087/1‑140 (MQ=255)                                                              ggTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTcat                                                                  >  1:347812/1‑140 (MQ=255)                                                              ggTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTcat                                                                  <  1:70660/140‑1 (MQ=255)                                                               gTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTcatc                                                                 >  2:819754/1‑140 (MQ=255)                                                                     ttGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCcatcat                                                           >  2:76497/1‑140 (MQ=255)                                                                       gAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATaa                                                         <  1:819754/140‑1 (MQ=255)                                                                        aGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAAt                                                        <  1:76497/140‑1 (MQ=255)                                                                         gCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATc                                                       >  2:216553/1‑140 (MQ=255)                                                                              tCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACAtt                                                  >  2:218169/1‑140 (MQ=255)                                                                                  tctGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCg                                              >  2:43579/1‑140 (MQ=255)                                                                                   ctGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGt                                             <  2:222977/140‑1 (MQ=255)                                                                                    tgatgaTTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTc                                            >  1:836018/1‑140 (MQ=255)                                                                                       tgatTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGa                                         >  2:928078/1‑140 (MQ=255)                                                                                           tAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATc                                     >  1:984403/1‑140 (MQ=255)                                                                                              ccATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCa                                  >  1:657075/1‑140 (MQ=255)                                                                                                  atCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATAAAATTTCCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTGGCGTCAGCAATCGCAAGGt                              >  2:193279/1‑140 (MQ=255)                                                                                                      gTACGCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGc                          <  1:218169/140‑1 (MQ=255)                                                                                                          gCATATAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAg                      <  1:878653/140‑1 (MQ=255)                                                                                                            atatAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGtt                    >  1:1082686/1‑140 (MQ=255)                                                                                                            atatAAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGtt                    >  2:464959/1‑140 (MQ=255)                                                                                                               taAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGt                              <  2:703580/127‑1 (MQ=255)                                                                                                               taAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGt                              >  1:703580/1‑127 (MQ=255)                                                                                                               taAAAATTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgag                 <  2:836018/140‑1 (MQ=255)                                                                                                                aaaaaTTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgagg                >  1:632618/1‑140 (MQ=255)                                                                                                                 aaaaTTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTATTGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgagga               <  1:893467/140‑1 (MQ=255)                                                                                                                 aaaaTTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgagga               <  1:43579/140‑1 (MQ=255)                                                                                                                    aTTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgagga               >  2:975132/1‑137 (MQ=255)                                                                                                                    aTTGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgagga               <  1:975132/137‑1 (MQ=255)                                                                                                                      tGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGgtg          >  2:613/1‑140 (MQ=255)                                                                                                                      tGCCGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGgtg          >  2:8566/1‑140 (MQ=255)                                                                                                                        ccGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgag                 <  1:447755/131‑1 (MQ=255)                                                                                                                        ccGTTTCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTgag                 >  2:447755/1‑131 (MQ=255)                                                                                                                           tttCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGGTGTTTTg     <  1:464959/140‑1 (MQ=255)                                                                                                                             tCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGGTGTTTTGGt   <  1:272087/140‑1 (MQ=255)                                                                                                                             tCCCGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGGTGTTTTGGt   <  2:745863/140‑1 (MQ=255)                                                                                                                              cccGACAGGGCATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGGTGTTTTGGtt  <  1:8566/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  CTGCTGCCGGGCTGGCACCGGTGTCCGTCACGAATATTTTGAAATCACCTGTTGCCTGACCGGTGACGTTGAGCTGATCGCTCTGATGATTAGCCATATCGGTACGCATATAAAAATTGCCGTTTCCCGACAGGGTATTGGTGGTCAACGTAATGAAATTTTCCGCACTTGCCGTCACTGATGAACGAGTCACTGGCTCATCCATCATAATCACATTTGCGTCAGACATCGCAAGGTTGGCTAAGTTGAGGAGGGTGTTTTGGTT  >  NC_000913/2287451‑2287715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑