Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,296,418	T→C	T203A (ACC→GCC)	ccmB ←	heme trafficking system membrane protein CcmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,296,418	0	T	C	100.0%	92.9 / NA	27	T203A (ACC→GCC)	ccmB	heme trafficking system membrane protein CcmB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

GCCGTGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTA  >  NC_000913/2296281‑2296537                                                                                                                                          |                                                                                                                        gCCGTGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGgcgc                                                                                                                       >  2:78145/1‑140 (MQ=255)            aTCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAc                                                                                                            >  1:761501/1‑140 (MQ=255)                ccAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACgcgc                                                                                                        <  2:761501/140‑1 (MQ=255)                 cAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGcc                                                                                                       <  2:1118229/140‑1 (MQ=255)                           aGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGcc                                                                                             <  2:950719/140‑1 (MQ=255)                             tgtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAg                                                                                           >  2:683620/1‑140 (MQ=255)                                       aTAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCa                                                                                 >  1:133434/1‑140 (MQ=255)                                       aTAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGATATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAGTTGCCAGATACCCGTCa                                                                                 >  2:1034685/1‑140 (MQ=255)                                         aGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAg                                                                                           <  1:449474/128‑1 (MQ=255)                                         aGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAg                                                                                           >  2:449474/1‑128 (MQ=255)                                         aGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAAc                                                                               >  2:558281/1‑140 (MQ=255)                                           tttCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACgg                                                                             >  1:311059/1‑140 (MQ=255)                                           tttCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACgg                                                                             >  2:156341/1‑140 (MQ=255)                                            ttCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACggg                                                                            <  1:164981/140‑1 (MQ=255)                                             tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                           <  1:828020/140‑1 (MQ=255)                                             tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                           <  1:78145/140‑1 (MQ=255)                                             tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                           <  1:696309/140‑1 (MQ=255)                                             tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                           <  1:558281/140‑1 (MQ=255)                                                      gACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATa                                                                  <  2:133434/140‑1 (MQ=255)                                                              cAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCcgc                                                          <  1:683620/140‑1 (MQ=255)                                                                              aGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTg                                          <  1:1003433/140‑1 (MQ=255)                                                                                       gAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATg                                 <  1:300300/140‑1 (MQ=255)                                                                                         aTGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAg                               <  1:1034685/140‑1 (MQ=255)                                                                                          tGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGt                              >  2:623027/1‑140 (MQ=255)                                                                                                 tCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGa                       <  1:313152/140‑1 (MQ=255)                                                                                                  cGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGAt                      >  2:107440/1‑140 (MQ=255)                                                                                                                cgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCATATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAaca        <  1:1016987/140‑1 (MQ=255)                                                                                                                      aaaaGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTa  >  2:588562/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                        GCCGTGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTA  >  NC_000913/2296281‑2296537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑