Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,363,290	T→C	N115S (AAC→AGC)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,363,290	0	T	C	100.0%	110.7 / NA	32	N115S (AAC→AGC)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

ACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGT  >  NC_000913/2363152‑2363417                                                                                                                                           |                                                                                                                                aCCATCACCTTAAATTCTGACGGTACGCC‑GGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTg                                                                                                                               >  1:241560/1‑140 (MQ=255)              aTTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTTGCAGGCAGCTCCGCTTGTTTACGGCTCCTCGGCGTCAtt                                                                                                                   >  2:701417/1‑140 (MQ=255)              aTTCTGACGGTACGCC‑GGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTa                                                                                                                  <  2:241560/140‑1 (MQ=255)                   gACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCg                                                                                                              >  2:213090/1‑140 (MQ=255)                       gTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTAc                                                                                                                 >  2:694366/1‑133 (MQ=255)                       gTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTAc                                                                                                                 <  1:694366/133‑1 (MQ=255)                       gTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCa                                                                                                          >  1:93905/1‑140 (MQ=255)                        tACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCAc                                                                                                         >  2:922935/1‑140 (MQ=255)                         aCGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACg                                                                                                        <  2:853661/140‑1 (MQ=255)                           gCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGtt                                                                                                      >  2:244388/1‑140 (MQ=255)                                gAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTgg                                                                                                 >  2:450678/1‑140 (MQ=255)                                   tAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGaaa                                                                                              <  1:213090/140‑1 (MQ=255)                                      aGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTGCACGTTCGTGGAAATAg                                                                                           <  1:789733/140‑1 (MQ=255)                                      aGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAg                                                                                           >  1:133230/1‑140 (MQ=255)                                        aaCATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCg                                                                                         <  1:353535/140‑1 (MQ=255)                                             cAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGtt                                                                                                      >  1:905478/1‑122 (MQ=255)                                             cAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGtt                                                                                                      <  2:905478/122‑1 (MQ=255)                                             cAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCCACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTTGTTACGGCTGCTTCGGGCCCTTACTCGCCCCCGATTGTGGGAATAACGTTcga                                                                                    >  2:789733/1‑138 (MQ=255)                                               ggCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCAt                                                                                   >  1:785014/1‑139 (MQ=255)                                               ggCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCAt                                                                                   <  2:785014/139‑1 (MQ=255)                                                gCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCAtct                                                                                 >  1:143275/1‑140 (MQ=255)                                                       ttAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAg                                                                          <  2:311260/140‑1 (MQ=255)                                                       ttAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAg                                                                          <  1:107951/140‑1 (MQ=255)                                                               cACGGCAAAACCACAGGCGGTGCCTACCGGGGTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCtt                                                                  >  2:572804/1‑140 (MQ=255)                                                                 cGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCa                                                                >  1:128558/1‑140 (MQ=255)                                                                   gCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATg                                                              <  2:143275/140‑1 (MQ=255)                                                                           acaGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCa                                                      <  1:450678/140‑1 (MQ=255)                                                                              ggCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGc                                                   >  1:1133353/1‑140 (MQ=255)                                                                                   tGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTcc                                              >  2:299368/1‑139 (MQ=255)                                                                                   tGCCTACCGGGGTATTGGTAAATTTAGCACTGGCAGGGAGCTCCGCTTGTTTACCGCTGCTCGGTGGCATTATTCATCCACGTTTGTGGAAATAGCGCTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTcc                                              >  2:678957/1‑139 (MQ=255)                                                                                        aCCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCttt                                         >  1:184847/1‑140 (MQ=255)                                                                                                          tCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAg                       <  1:572804/140‑1 (MQ=255)                                                                                                           cAGCACTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGt                      <  2:128558/140‑1 (MQ=255)                                                                                                                cTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCt                 <  2:184847/140‑1 (MQ=255)                                                                                                                cTGGCAGGCAGCTCCGCTTGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCt                 <  1:922935/140‑1 (MQ=255)                                                                                                                                 ttGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggt  <  2:365002/138‑1 (MQ=255)                                                                                                                                 ttGTTTACGGCTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggt  >  1:365002/1‑138 (MQ=255)                                                                                                                                           |                                                                                                                                ACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGT  >  NC_000913/2363152‑2363417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑