Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,440,439	G→A	S389S (AGC→AGT)	fabB ←	beta‑ketoacyl‑[acyl carrier protein] synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,440,439	0	G	A	100.0%	173.7 / NA	50	S389S (AGC→AGT)	fabB	beta‑ketoacyl‑[acyl carrier protein] synthase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

CCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAG  >  NC_000913/2440303‑2440574                                                                                                                                         |                                                                                                                                        cccTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTg                                                                                                                                      >  2:440070/1‑140 (MQ=255)   cTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGtt                                                                                                                                    <  2:155996/140‑1 (MQ=255)   cTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGtt                                                                                                                                    <  2:404243/140‑1 (MQ=255)    ttttGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTa                                                                                                                                   >  2:971599/1‑140 (MQ=255)      ttGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGa                                                                                                                                 >  2:591954/1‑140 (MQ=255)              gTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTa                                                                                                                                   >  2:366423/1‑130 (MQ=255)              gTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTa                                                                                                                                   <  1:366423/130‑1 (MQ=255)              gTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACgg                                                                                                                         >  1:1021217/1‑140 (MQ=255)                  cGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACggtggt                                                                                                                     <  2:511494/140‑1 (MQ=255)                  cGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACggtggt                                                                                                                     <  1:1017381/140‑1 (MQ=255)                  cGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACggtggt                                                                                                                     <  2:1021217/140‑1 (MQ=255)                         cGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTcg                                                                                                              >  1:115202/1‑140 (MQ=255)                         cGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTcg                                                                                                              <  1:591954/140‑1 (MQ=255)                                 cgcgTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGt                                                                                                      >  1:393374/1‑140 (MQ=255)                                         gTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCgg                                                                                              <  1:440070/140‑1 (MQ=255)                                           cGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTAGTTTCGGTc                                                                                            >  1:719452/1‑140 (MQ=255)                                                   aCGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCGGTTCGCGATCGGTCGTTTCGGTCACg                                                                                         >  2:898330/1‑135 (MQ=255)                                                   aCGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCGGTTCGCGATCGGTCGTTTCGGTCACg                                                                                         <  1:898330/135‑1 (MQ=255)                                                           gcgTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCg                                                                            <  1:586082/140‑1 (MQ=255)                                                                    ccGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCt                                                                   >  2:616211/1‑140 (MQ=255)                                                                     cGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTc                                                                  >  2:883329/1‑140 (MQ=255)                                                                      gACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCg                                                                 <  1:1007208/140‑1 (MQ=255)                                                                       aCCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGt                                                                >  1:176240/1‑140 (MQ=255)                                                                       aCCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTACCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGt                                                                >  1:573126/1‑140 (MQ=255)                                                                        ccTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTc                                                               >  1:861313/1‑140 (MQ=255)                                                                          tACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCa                                                             >  2:857338/1‑140 (MQ=255)                                                                           aCTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAg                                                            <  2:1120091/140‑1 (MQ=255)                                                                            cTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGc                                                           >  1:1082412/1‑140 (MQ=255)                                                                               cGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGctct                                                        >  1:965827/1‑140 (MQ=255)                                                                                 aaTTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAg                                                            >  1:1152766/1‑134 (MQ=255)                                                                                 aaTTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAg                                                            <  2:1152766/134‑1 (MQ=255)                                                                                 aaTTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCCAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTc                                                      <  2:510267/140‑1 (MQ=255)                                                                                   ttAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCaa                                                    <  1:883329/140‑1 (MQ=255)                                                                                            cAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGc                                           >  1:1122464/1‑140 (MQ=255)                                                                                             aGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCt                                          <  1:971599/140‑1 (MQ=255)                                                                                             aGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCt                                          <  2:393374/140‑1 (MQ=255)                                                                                             aGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCt                                          <  1:857338/140‑1 (MQ=255)                                                                                                ttGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCgg                                       >  1:675566/1‑140 (MQ=255)                                                                                                ttGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCgg                                       <  2:782807/140‑1 (MQ=255)                                                                                                            aGCGTGGCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGcc                           <  2:14619/140‑1 (MQ=255)                                                                                                                  gCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTc                     >  2:956095/1‑140 (MQ=255)                                                                                                                  gCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTc                     >  2:305096/1‑140 (MQ=255)                                                                                                                  gCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTc                     >  1:457276/1‑140 (MQ=255)                                                                                                                  gCGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTc                     >  1:699704/1‑140 (MQ=255)                                                                                                                   cGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTcc                    <  2:457276/140‑1 (MQ=255)                                                                                                                   cGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTcc                    <  1:583122/140‑1 (MQ=255)                                                                                                                   cGTTGGTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGCTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTcc                    <  1:1016564/140‑1 (MQ=255)                                                                                                                        gTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCAt               >  2:822371/1‑140 (MQ=255)                                                                                                                        gTGCCGCCGAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCAt               <  2:965827/140‑1 (MQ=255)                                                                                                                            cgccgAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATcagc           <  1:241370/140‑1 (MQ=255)                                                                                                                            cgccgAAGCCGAAACTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATcagc           <  1:305096/140‑1 (MQ=255)                                                                                                                                 aagccgaaacTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCagaga      <  2:115202/140‑1 (MQ=255)                                                                                                                                     cgaaacTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTTGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGc                 <  1:246356/125‑1 (MQ=255)                                                                                                                                     cgaaacTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTTGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGc                 >  2:246356/1‑125 (MQ=255)                                                                                                                                     cgaaacTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAg  <  1:616211/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTTAGACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAG  >  NC_000913/2440303‑2440574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑