| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,448,697 | T→C | V31A (GTC→GCC) | smrB → | putative endonuclease SmrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,448,697 | 0 | T | C | 94.7% | 121.8 / ‑1.9 | 38 | V31A (GTC→GCC) | smrB | putative endonuclease SmrB |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (18/18); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATG > NC_000913/2448572‑2448828 | aaaCTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGc < 2:385542/140‑1 (MQ=255) ttAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGc > 1:1157789/1‑140 (MQ=255) aGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCa < 1:492774/122‑1 (MQ=255) aGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCa > 2:492774/1‑122 (MQ=255) cAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGa < 2:729662/140‑1 (MQ=255) aGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGaa < 2:631231/140‑1 (MQ=255) aGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGaa < 1:226944/140‑1 (MQ=255) aTAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAAc > 2:992414/1‑140 (MQ=255) aTAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAAc > 2:1163014/1‑140 (MQ=255) aaGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCa > 1:72273/1‑140 (MQ=255) aaaCAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAg < 2:72273/140‑1 (MQ=255) aaaCAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAg < 2:1157789/140‑1 (MQ=255) aacaacACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGc > 1:244767/1‑140 (MQ=255) cacTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCt < 1:528316/140‑1 (MQ=255) gaggagGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgccag > 2:279739/1‑140 (MQ=255) gaggagGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgccag > 2:199871/1‑140 (MQ=255) aggaggACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgccagc < 1:83110/140‑1 (MQ=255) gaCCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCatta > 2:97985/1‑140 (MQ=255) ggCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTtct > 1:204053/1‑140 (MQ=255) ggCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTtct < 2:204053/140‑1 (MQ=255) gCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTtctc > 2:409674/1‑140 (MQ=255) tGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGAt > 1:1135847/1‑140 (MQ=255) tGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGAt > 1:94349/1‑140 (MQ=255) gTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATg < 1:1163014/140‑1 (MQ=255) gTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGc < 1:279739/140‑1 (MQ=255) gTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGc < 1:409674/140‑1 (MQ=255) gTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGc > 2:894192/1‑140 (MQ=255) tGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCg < 1:199871/140‑1 (MQ=255) tGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCg < 2:94349/140‑1 (MQ=255) gggACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTaaa > 2:603116/1‑140 (MQ=255) gggACTCGCAAGATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTaaa < 1:97985/140‑1 (MQ=255) gATTAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTc < 2:593734/140‑1 (MQ=255) ttAAGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCg < 2:1135847/140‑1 (MQ=255) tAAGCAGGACACGATTGCCCAGCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCTTTATTAAATACCGAAGGTCCgg < 1:80391/140‑1 (MQ=255) aaGCAGGACACGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGt > 1:851187/1‑140 (MQ=255) acacGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAatat > 1:811763/1‑140 (MQ=255) cacGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATg > 2:1045522/1‑140 (MQ=255) cacGATTGCCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATg > 1:1138442/1‑140 (MQ=255) | AAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATG > NC_000913/2448572‑2448828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |