Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,259	A→G	H20R (CAC→CGC)	yagF →	CP4‑6 prophage; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,259	0	A	G	100.0%	173.1 / NA	48	H20R (CAC→CGC)	yagF	CP4‑6 prophage; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/30);  total (18/30)

CGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGT  >  NC_000913/283125‑283391                                                                                                                                       |                                                                                                                                     cgcTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCgcgg                                                                                                                                 <  2:863181/140‑1 (MQ=255)   cTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCgcggcg                                                                                                                               <  1:482306/140‑1 (MQ=255)           gCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCa                                                                                                                       >  2:59924/1‑140 (MQ=255)               cgcAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAggg                                                                                                                    <  1:1088453/139‑1 (MQ=255)               cgcAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAggg                                                                                                                    >  2:1088453/1‑139 (MQ=255)                  aaGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGct                                                                                                                <  2:409004/140‑1 (MQ=255)                  aaGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGct                                                                                                                <  1:369866/140‑1 (MQ=255)                        cAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCg                                                                                                          <  1:59924/140‑1 (MQ=255)                         aGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGc                                                                                                         >  2:734850/1‑140 (MQ=255)                          gCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCt                                                                                                        <  1:352092/140‑1 (MQ=255)                             gAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGAc                                                                                                     <  1:654474/140‑1 (MQ=255)                              aaaaCCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGAcc                                                                                                    <  1:1113718/140‑1 (MQ=255)                                      gctgcAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGAt                                                                                            <  1:719997/140‑1 (MQ=255)                                        tgcAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGc                                                                                          >  2:336549/1‑140 (MQ=255)                                           aaCAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGa                                                                                       <  1:996864/140‑1 (MQ=255)                                                     gCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTcc                                                                             <  2:153245/140‑1 (MQ=255)                                                               gCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAAc                                                                   >  1:12510/1‑140 (MQ=255)                                                                     cGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTc                                                             >  1:870770/1‑140 (MQ=255)                                                                        tAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGc                                                          >  2:602677/1‑140 (MQ=255)                                                                              tGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACg                                                    >  2:176523/1‑140 (MQ=255)                                                                                aCCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa                                                  >  1:1056396/1‑140 (MQ=255)                                                                                aCCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa                                                  <  2:1056396/140‑1 (MQ=255)                                                                                aCCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa                                                  <  2:772018/140‑1 (MQ=255)                                                                                aCCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa                                                  <  2:870770/140‑1 (MQ=255)                                                                                    ttGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAAc                                              >  1:689935/1‑140 (MQ=255)                                                                                     tGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACg                                             >  2:542920/1‑140 (MQ=255)                                                                                         aaaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCgg                                         >  1:544754/1‑140 (MQ=255)                                                                                          aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa                                                  <  2:564760/130‑1 (MQ=255)                                                                                          aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa                                                  >  1:564760/1‑130 (MQ=255)                                                                                          aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCggg                                        <  1:542920/140‑1 (MQ=255)                                                                                          aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCggg                                        <  1:336549/140‑1 (MQ=255)                                                                                           aaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGa                                       <  2:689935/140‑1 (MQ=255)                                                                                           aaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGa                                       <  1:734850/140‑1 (MQ=255)                                                                                            aTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGAt                                      >  2:926136/1‑140 (MQ=255)                                                                                                  cccccGCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTg                                <  2:855925/139‑1 (MQ=255)                                                                                                       gCAGGACGACGCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACg                           <  2:12510/140‑1 (MQ=255)                                                                                                             cgacgCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCcaaca                     >  1:195651/1‑140 (MQ=255)                                                                                                               acgCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAg                   <  2:195651/140‑1 (MQ=255)                                                                                                               acgCGTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAg                   <  2:553948/140‑1 (MQ=255)                                                                                                                cgcgTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGc                  >  1:41262/1‑140 (MQ=255)                                                                                                                 gcgTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCt                 <  2:235344/140‑1 (MQ=255)                                                                                                                 gcgTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCt                 <  1:926136/140‑1 (MQ=255)                                                                                                                 gcgTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCt                 <  1:602677/140‑1 (MQ=255)                                                                                                                  cgTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTc                >  1:501689/1‑140 (MQ=255)                                                                                                                   gTTTTATGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCa               <  1:629208/140‑1 (MQ=255)                                                                                                                        aTGCGGTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGc          <  2:544754/140‑1 (MQ=255)                                                                                                                            ggTGATCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAag      >  1:663564/1‑140 (MQ=255)                                                                                                                                aTCACCCGCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGt  <  1:912102/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGT  >  NC_000913/283125‑283391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑