Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,597,390	T→C	T77A (ACC→GCC)	purC ←	phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,597,390	0	T	C	100.0%	224.2 / NA	61	T77A (ACC→GCC)	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/23);  total (38/23)

ACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGT  >  NC_000913/2597258‑2597525                                                                                                                                     |                                                                                                                                        aCAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGgag                                                                                                                                  <  1:532058/140‑1 (MQ=255) aCAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGgag                                                                                                                                  <  1:1041368/140‑1 (MQ=255)      tCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAg                                                                                                                             <  2:341492/140‑1 (MQ=255)               ggcggGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCAt                                                                                                                    <  2:379707/140‑1 (MQ=255)                    gTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAg                                                                                                               <  1:1010692/140‑1 (MQ=255)                        aGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCAttt                                                                                                                  >  1:578908/1‑133 (MQ=255)                        aGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCAttt                                                                                                                  <  2:578908/133‑1 (MQ=255)                               tACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATAccc                                                                                                    >  2:177309/1‑140 (MQ=255)                                        cGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGcc                                                                                           >  1:880606/1‑140 (MQ=255)                                         gATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCa                                                                                          >  1:264686/1‑140 (MQ=255)                                           ttCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGt                                                                                        >  2:1094905/1‑140 (MQ=255)                                           ttCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGt                                                                                        >  2:879215/1‑140 (MQ=255)                                           ttCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGt                                                                                        <  2:827380/140‑1 (MQ=255)                                                aGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCt                                                                                   <  1:879215/140‑1 (MQ=255)                                                aGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCt                                                                                   >  2:1111355/1‑140 (MQ=255)                                                              agagCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAagtagt                                                                     >  2:254373/1‑140 (MQ=255)                                                                agCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAagtagt                                                                     <  2:251285/138‑1 (MQ=255)                                                                agCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAagtagt                                                                     >  1:251285/1‑138 (MQ=255)                                                                agCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTg                                                                   >  2:78599/1‑140 (MQ=255)                                                                         caCGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                          <  1:1111355/140‑1 (MQ=255)                                                                         caCGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                          <  1:912662/140‑1 (MQ=255)                                                                           cGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTGCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTgttgtt                                                        <  1:644766/140‑1 (MQ=255)                                                                            ggTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTc                                                       >  2:998963/1‑140 (MQ=255)                                                                             gTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCa                                                      <  2:264686/140‑1 (MQ=255)                                                                                 cGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCAt                                                  >  1:179586/1‑140 (MQ=255)                                                                                      gACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCtt                                             >  1:776144/1‑140 (MQ=255)                                                                                        cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                          >  1:870051/1‑125 (MQ=255)                                                                                        cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                          <  2:870051/125‑1 (MQ=255)                                                                                        cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg                                           >  2:868711/1‑140 (MQ=255)                                                                                        cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg                                           >  1:805440/1‑140 (MQ=255)                                                                                        cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg                                           >  1:275778/1‑140 (MQ=255)                                                                                        cacacTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCACTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg                                           >  1:466301/1‑140 (MQ=255)                                                                                           acTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                          >  1:958266/1‑122 (MQ=255)                                                                                           acTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                                                          <  2:958266/122‑1 (MQ=255)                                                                                                    gCACCATATCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGc                               >  2:185970/1‑140 (MQ=255)                                                                                                         atatCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAAt                          >  2:800034/1‑140 (MQ=255)                                                                                                         atatCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAAt                          >  2:492804/1‑140 (MQ=255)                                                                                                            tCCAGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcg                       <  1:177309/140‑1 (MQ=255)                                                                                                               aGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGTTCAATgcgcgc                    >  1:489904/1‑140 (MQ=255)                                                                                                               aGCTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcgcgc                    >  1:1062494/1‑140 (MQ=255)                                                                                                                 cTTTTTCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcgcgcgc                  >  2:962945/1‑140 (MQ=255)                                                                                                                  tttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGcc                 >  2:1070136/1‑140 (MQ=255)                                                                                                                  tttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGcc                 >  2:766481/1‑140 (MQ=255)                                                                                                                  tttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGcc                 >  1:65882/1‑140 (MQ=255)                                                                                                                  tttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGcc                 >  2:1144859/1‑140 (MQ=255)                                                                                                                  tttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGcc                 >  1:91911/1‑140 (MQ=255)                                                                                                                  tttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGcc                 >  1:965723/1‑140 (MQ=255)                                                                                                                   ttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCa                >  2:338945/1‑140 (MQ=255)                                                                                                                   ttttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCa                >  2:82282/1‑140 (MQ=255)                                                                                                                    tttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCAt               <  1:1152816/140‑1 (MQ=255)                                                                                                                    tttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCAt               >  1:211947/1‑140 (MQ=255)                                                                                                                    tttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCAt               >  2:902700/1‑140 (MQ=255)                                                                                                                    tttCACCAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCAt               >  2:636949/1‑140 (MQ=255)                                                                                                                         ccAGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCct          <  2:91911/140‑1 (MQ=255)                                                                                                                           aGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCctgc        <  2:1062494/140‑1 (MQ=255)                                                                                                                           aGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCctgc        <  2:65882/140‑1 (MQ=255)                                                                                                                           aGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCctgc        <  1:338945/140‑1 (MQ=255)                                                                                                                           aGACATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCACTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCctgc        <  2:466301/140‑1 (MQ=255)                                                                                                                             aCATTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCctgctg      <  1:962945/140‑1 (MQ=255)                                                                                                                               aTTCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGAc    >  2:505380/1‑140 (MQ=255)                                                                                                                                 tCGGCATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGt  >  1:1021234/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGT  >  NC_000913/2597258‑2597525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑