Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,713	T→C	D25G (GAC→GGC)	kbp ←	K(+) binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,713	0	T	C	100.0%	195.4 / NA	54	D25G (GAC→GGC)	kbp	K(+) binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/29);  total (25/29)

TTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGTCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACG  >  NC_000913/2796579‑2796849                                                                                                                                       |                                                                                                                                         ttttCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTa                                                                                                                                     <  2:813015/140‑1 (MQ=255)         ttCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTg                                                                                                                             <  2:59789/140‑1 (MQ=255)                    aCTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACcg                                                                                                                  <  2:543039/140‑1 (MQ=255)                          gCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTccc                                                                                                            >  1:339915/1‑140 (MQ=255)                           ccGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCa                                                                                                           <  1:22452/140‑1 (MQ=255)                           ccGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCa                                                                                                           <  1:700766/140‑1 (MQ=255)                                aCCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGtt                                                                                                      >  2:295216/1‑140 (MQ=255)                                         cGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCgg                                                                                             >  2:790606/1‑140 (MQ=255)                                         cGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCgg                                                                                             >  2:1050514/1‑140 (MQ=255)                                          gTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGc                                                                                            >  2:132983/1‑140 (MQ=255)                                            cGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCAt                                                                                          >  2:737627/1‑140 (MQ=255)                                               tttGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCtt                                                                                       >  1:651868/1‑140 (MQ=255)                                                  gCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTca                                                                                    <  1:1050514/140‑1 (MQ=255)                                                  gCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTca                                                                                    <  2:818456/140‑1 (MQ=255)                                                            aTTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGaa                                                                          <  2:994575/140‑1 (MQ=255)                                                            aTTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGaa                                                                          <  1:212765/140‑1 (MQ=255)                                                             tttGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAAc                                                                         <  1:522752/140‑1 (MQ=255)                                                             tttGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAAc                                                                         <  2:768462/140‑1 (MQ=255)                                                             tttGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAAc                                                                         <  2:428626/140‑1 (MQ=255)                                                              ttGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACa                                                                        <  2:687090/140‑1 (MQ=255)                                                               tGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAg                                                                       <  2:651868/140‑1 (MQ=255)                                                                 aaTATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGAc                                                                     <  1:790606/140‑1 (MQ=255)                                                                  atatTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGAcc                                                                    <  2:493407/140‑1 (MQ=255)                                                                   tatTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGAccc                                                                   <  1:513775/140‑1 (MQ=255)                                                                       cACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAg                                                               <  1:463646/140‑1 (MQ=255)                                                                        aCTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGc                                                              >  1:1004534/1‑140 (MQ=255)                                                                        aCTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGc                                                              <  1:295216/140‑1 (MQ=255)                                                                           ttATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCACAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTAc                                                           >  2:80226/1‑140 (MQ=255)                                                                           ttATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGACTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTAc                                                           >  2:485671/1‑140 (MQ=255)                                                                                    tCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGt                                                  >  1:322748/1‑140 (MQ=255)                                                                                      cGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTa                                                >  1:118517/1‑140 (MQ=255)                                                                                           taCCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGa                                           <  2:118517/140‑1 (MQ=255)                                                                                              cGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGa                                           <  1:501372/137‑1 (MQ=255)                                                                                              cGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGa                                           >  2:501372/1‑137 (MQ=255)                                                                                              cGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAac                                        >  1:300449/1‑140 (MQ=255)                                                                                              cGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAac                                        >  1:1076535/1‑140 (MQ=255)                                                                                                gTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTaa                                               <  1:880958/131‑1 (MQ=255)                                                                                                gTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTaa                                               >  2:880958/1‑131 (MQ=255)                                                                                                   ttGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACCCTCa                                   <  2:339915/140‑1 (MQ=255)                                                                                                     gTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCa                                   <  2:563560/138‑1 (MQ=255)                                                                                                     gTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCa                                   >  1:563560/1‑138 (MQ=255)                                                                                                     gTTCAGATGCTCCTGCACCTTCTTCGACTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGa                                 <  1:485671/140‑1 (MQ=255)                                                                                                      ttCAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGaa                                >  2:15541/1‑140 (MQ=255)                                                                                                        cAGATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAg                              <  2:1076535/140‑1 (MQ=255)                                                                                                          gATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAgtg                            <  1:15541/140‑1 (MQ=255)                                                                                                          gATGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAgtg                            >  1:583444/1‑140 (MQ=255)                                                                                                           aTGCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAgtgt                           >  1:229728/1‑140 (MQ=255)                                                                                                             gCTCCTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAg                         <  2:229728/140‑1 (MQ=255)                                                                                                                ccTGCACCTTCTTCGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCag                      >  2:1001184/1‑140 (MQ=255)                                                                                                                         tcttcGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAAcgcg             >  2:1028317/1‑140 (MQ=255)                                                                                                                         tcttcGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGCTAACGGAAACACTCAGAAAGTGTAGCAGAGGAAcgcg             >  2:1015100/1‑140 (MQ=255)                                                                                                                           ttcGCCTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACgcggc           >  1:805849/1‑140 (MQ=255)                                                                                                                                cTGATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGtt      >  1:1100891/1‑140 (MQ=255)                                                                                                                                  gATCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTaa    <  2:639454/140‑1 (MQ=255)                                                                                                                                    tCGCCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAAcg  <  1:75326/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTTATCGGCATCCGGTATACCGGTTTTGTTCAGATGCTCCTGCACCTTCTTCGCCTGATCGTCTTTATCGTGCTGACCTGTAACCGCGTCCCAGAGTTTTTCTCCGGCATCTTTCACAAAATTGAACAGACCCATAGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACG  >  NC_000913/2796579‑2796849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑