Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,843,851	C→T	G120G (GGG→GGA)	hycG ←	formate hydrogenlyase subunit HycG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,843,851	0	C	T	100.0%	192.5 / NA	54	G120G (GGG→GGA)	hycG	formate hydrogenlyase subunit HycG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/26);  total (28/26)

TCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGT  >  NC_000913/2843715‑2843986                                                                                                                                         |                                                                                                                                        tCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCg                                                                                                                                      >  2:1147168/1‑140 (MQ=255)       ccAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTg                                                                                                                                <  1:91676/140‑1 (MQ=255)         agcagGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGtt                                                                                                                              <  1:829108/140‑1 (MQ=255)               ccGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCa                                                                                                                        <  2:1102880/140‑1 (MQ=255)                 gAGCGCCATTGCAAAGCCGTACAGCGTGGTGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGGACCGCCCCACACGCAGTAGAGATCGTGGAAGAGACCGCCACTGTTGCCGCAgg                                                                                                                      <  2:921397/140‑1 (MQ=255)                                                      gTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCTGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGa                                                                                 <  1:415139/140‑1 (MQ=255)                                                        tGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACt                                                                               <  1:842853/140‑1 (MQ=255)                                                                  ccAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacgcacg                                                                     <  2:1028465/140‑1 (MQ=255)                                                                  ccAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacgcacg                                                                     <  2:242513/140‑1 (MQ=255)                                                                     gggATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCAcgcag                                                                  >  2:1076574/1‑140 (MQ=255)                                                                                aTCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                                         >  2:333634/1‑138 (MQ=255)                                                                                aTCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                                         <  1:333634/138‑1 (MQ=255)                                                                                aTCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATc                                                       >  2:952953/1‑140 (MQ=255)                                                                                aTCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATc                                                       >  2:1108483/1‑140 (MQ=255)                                                                                 tCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACGGCAGGCACCGTAGGAGAAACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATcc                                                      >  2:1041350/1‑139 (MQ=255)                                                                                 tCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCg                                                      <  1:1108483/140‑1 (MQ=255)                                                                                 tCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCg                                                      <  2:754775/140‑1 (MQ=255)                                                                                  ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGAt                                                        >  2:901815/1‑137 (MQ=255)                                                                                  ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGAt                                                        <  1:33067/137‑1 (MQ=255)                                                                                  ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGAt                                                        >  2:33067/1‑137 (MQ=255)                                                                                  ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGAt                                                        <  1:901815/137‑1 (MQ=255)                                                                                  ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCg                                                      >  1:760302/1‑139 (MQ=255)                                                                                  ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCg                                                      <  2:760302/139‑1 (MQ=255)                                                                                    acaGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCAt                                                   >  1:120751/1‑140 (MQ=255)                                                                                     caGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCTCAtt                                                  >  2:1070406/1‑140 (MQ=255)                                                                                        ggACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCa                                               <  2:120751/140‑1 (MQ=255)                                                                                        ggACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCa                                               >  1:35924/1‑140 (MQ=255)                                                                                             aTTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGt                                          >  2:802950/1‑140 (MQ=255)                                                                                                  aTCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACcg                                     <  2:656522/140‑1 (MQ=255)                                                                                                  aTCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACcg                                     <  1:460466/140‑1 (MQ=255)                                                                                                           gCCCCACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaa                            <  1:802950/140‑1 (MQ=255)                                                                                                            ccccACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaaa                           >  1:980413/1‑140 (MQ=255)                                                                                                            ccccACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaaa                           >  1:194769/1‑140 (MQ=255)                                                                                                            ccccACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaaa                           >  1:1116534/1‑140 (MQ=255)                                                                                                            ccccACACGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCCCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaaa                           <  1:100091/140‑1 (MQ=255)                                                                                                               cacaCGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTa                             >  2:1085513/1‑135 (MQ=255)                                                                                                               cacaCGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTa                             <  1:1085513/135‑1 (MQ=255)                                                                                                                 cacGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTa                      <  2:980413/140‑1 (MQ=255)                                                                                                                 cacGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTa                      <  2:778233/140‑1 (MQ=255)                                                                                                                 cacGCAGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTa                      <  2:438933/140‑1 (MQ=255)                                                                                                                      aGTAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAata                 >  1:245842/1‑140 (MQ=255)                                                                                                                        tAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATc               >  2:796651/1‑140 (MQ=255)                                                                                                                        tAGAGATCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATc               >  1:65647/1‑140 (MQ=255)                                                                                                                             aTCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCAt          >  2:189437/1‑140 (MQ=255)                                                                                                                              tCGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATg         >  1:920294/1‑140 (MQ=255)                                                                                                                               cGTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGa        >  2:1032661/1‑140 (MQ=255)                                                                                                                                gTGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAc       >  1:559588/1‑140 (MQ=255)                                                                                                                                 tGGAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAcg      <  1:1076574/140‑1 (MQ=255)                                                                                                                                  ggAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCa           >  1:958125/1‑134 (MQ=255)                                                                                                                                  ggAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCa           <  2:958125/134‑1 (MQ=255)                                                                                                                                  ggAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAcgc     >  2:561885/1‑140 (MQ=255)                                                                                                                                  ggAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAcgc     >  1:770912/1‑140 (MQ=255)                                                                                                                                  ggAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAcgc     >  1:212299/1‑140 (MQ=255)                                                                                                                                   gAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAcgcg    <  2:35924/140‑1 (MQ=255)                                                                                                                                   gAAGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAcgcg    <  2:212299/140‑1 (MQ=255)                                                                                                                                     aGATTCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGt  >  1:773783/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGT  >  NC_000913/2843715‑2843986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑