Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,943,333	T→C	intergenic (‑188/‑4)	ygdI ← / → csdA	DUF903 domain‑containing lipoprotein YgdI/cysteine sulfinate desulfinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,943,333	0	T	C	100.0%	214.5 / NA	60	intergenic (‑188/‑4)	ygdI/csdA	DUF903 domain‑containing lipoprotein YgdI/cysteine sulfinate desulfinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

TAAAATTAACGCCTAACACTATTCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGTACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAGTTTT  >  NC_000913/2943197‑2943463                                                                                                                                         |                                                                                                                                   tAAAATTAACGCCTAACACTATTCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCAcc                                                                                                                                 >  1:1064169/1‑140 (MQ=255)       tAACGCCTAACACTATTCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAAc                                                                                                                           <  1:435522/140‑1 (MQ=255)       tAACGCCTAACACTATTCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAAc                                                                                                                           <  1:1155166/140‑1 (MQ=255)               aaCACTATTCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAGGCCGAGGAGCACCATGAACGTTTTTaa                                                                                                                   >  2:1118606/1‑140 (MQ=255)                      ttCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCcgcg                                                                                                            >  2:829784/1‑140 (MQ=255)                       tCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCgcgc                                                                                                           >  2:939977/1‑140 (MQ=255)                                gTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTcgcg                                                                                                  >  1:1136450/1‑140 (MQ=255)                                gTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTcgcg                                                                                                  >  2:1089955/1‑140 (MQ=255)                                gTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTcgcg                                                                                                  >  1:928083/1‑140 (MQ=255)                                gTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTcgcg                                                                                                  >  1:643796/1‑140 (MQ=255)                                  tacttacGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGcc                                                                                                <  1:1089955/140‑1 (MQ=255)                                  tacttacGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGcc                                                                                                <  2:1064169/140‑1 (MQ=255)                                   acttacGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGccc                                                                                               >  1:580590/1‑140 (MQ=255)                                    cttacGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCa                                                                                              <  1:829784/140‑1 (MQ=255)                                         gcgGCTTCGCCAGGATATCCAGCTAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTAACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTc                                                                                         >  2:1118525/1‑140 (MQ=255)                                         gcgGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTc                                                                                         >  2:859088/1‑140 (MQ=255)                                         gcgGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTc                                                                                         <  1:859088/140‑1 (MQ=255)                                         gcgGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTc                                                                                         >  2:969753/1‑140 (MQ=255)                                           ggCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTccc                                                                                       <  1:939977/140‑1 (MQ=255)                                            gCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAGGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCg                                                                                      <  1:1118606/140‑1 (MQ=255)                                                  ccAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTc                                                                                         >  2:219863/1‑131 (MQ=255)                                                  ccAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTc                                                                                         <  1:219863/131‑1 (MQ=255)                                                          tCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCg                                                                        <  2:125252/140‑1 (MQ=255)                                                          tCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCg                                                                        <  1:969753/140‑1 (MQ=255)                                                              gATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCg                                                                    <  1:186492/140‑1 (MQ=255)                                                               aTAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCGCTACAGGATGCGGGCg                                                                    <  1:795145/139‑1 (MQ=255)                                                               aTAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCg                                                                    >  2:795145/1‑139 (MQ=255)                                                                tAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTc                                                                  >  2:100984/1‑140 (MQ=255)                                                                   ttCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTAt                                                               <  2:240581/140‑1 (MQ=255)                                                                    tCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCg                                                                    <  2:1148667/134‑1 (MQ=255)                                                                    tCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCg                                                                    >  1:1148667/1‑134 (MQ=255)                                                                    tCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTAtc                                                              >  2:1108906/1‑140 (MQ=255)                                                                    tCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCACGCACTACAGGATGCGGGCGTCTAtc                                                              >  2:4252/1‑140 (MQ=255)                                                                        aTGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGa                                                          >  1:411053/1‑140 (MQ=255)                                                                         tGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGAc                                                         >  1:287240/1‑140 (MQ=255)                                                                         tGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGAc                                                         >  2:469681/1‑140 (MQ=255)                                                                         tGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGAc                                                         <  2:411053/140‑1 (MQ=255)                                                                                     tcCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGAcc                                             >  1:32315/1‑140 (MQ=255)                                                                                     tcCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGAcc                                             >  2:376192/1‑140 (MQ=255)                                                                                       cTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACcgc                                           >  1:739250/1‑140 (MQ=255)                                                                                               aaaGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAAc                                   >  2:500414/1‑140 (MQ=255)                                                                                                 aGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCt                                 >  1:21314/1‑140 (MQ=255)                                                                                                 aGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCt                                 >  2:173571/1‑140 (MQ=255)                                                                                                  ttGAATTTTGCGTCATGATCGGTGCATCAAGCCGAGGAGCACTATGAACGTTTTTAATCCTGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGAATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTg                                <  2:580590/139‑1 (MQ=255)                                                                                                   tGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATACGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGa                               >  1:145710/1‑140 (MQ=255)                                                                                                      aTTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGc                            <  1:469681/140‑1 (MQ=255)                                                                                                       ttttGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGcc                           <  1:100984/140‑1 (MQ=255)                                                                                                        tttGCGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCg                          <  1:500414/140‑1 (MQ=255)                                                                                                            cGTCACGATCGGTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGt                      <  1:1108906/140‑1 (MQ=255)                                                                                                                       gTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCAccc           <  1:376192/140‑1 (MQ=255)                                                                                                                       gTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCAccc           <  2:643796/140‑1 (MQ=255)                                                                                                                       gTGCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCAccc           <  2:928083/140‑1 (MQ=255)                                                                                                                         gCATCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATACGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCaa         <  2:145710/140‑1 (MQ=255)                                                                                                                           aTCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACa       <  1:1129154/140‑1 (MQ=255)                                                                                                                            tCAAGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAg      >  2:47884/1‑140 (MQ=255)                                                                                                                              aaGCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAGtt    >  1:772958/1‑140 (MQ=255)                                                                                                                                gCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGGGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAGtttt  >  1:427551/1‑140 (MQ=255)                                                                                                                                gCCGAGGAGCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAGtttt  >  2:964260/1‑140 (MQ=255)                                                                                                                                    aggagCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCaa         >  2:2051/1‑129 (MQ=255)                                                                                                                                    aggagCACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCaa         <  1:2051/129‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TAAAATTAACGCCTAACACTATTCAGCATATGTTACTTACGCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGTGCATCAAGCCGAGGAGTACCATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAGTTTT  >  NC_000913/2943197‑2943463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑