Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,965,981	T→C	G19G (GGA→GGG)	lgt ←	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,981	0	T	C	100.0%	184.0 / NA	51	G19G (GGA→GGG)	lgt	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/20);  total (31/20)

AGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGTCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAG  >  NC_000913/2965845‑2966117                                                                                                                                         |                                                                                                                                         aGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCt                                                                                                                                       >  2:138759/1‑140 (MQ=255)    ccGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTAtt                                                                                                                                    >  2:8352/1‑140 (MQ=255)    ccGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTAtt                                                                                                                                    >  2:1078957/1‑140 (MQ=255)     cGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCTCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTg                                                                                                                                   <  2:34323/140‑1 (MQ=255)     cGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTg                                                                                                                                   >  1:917770/1‑140 (MQ=255)        aTAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTgaga                                                                                                                                >  2:49716/1‑140 (MQ=255)            agTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAAt                                                                                                                            >  1:486516/1‑140 (MQ=255)              tAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGa                                                                                                                          >  2:223011/1‑140 (MQ=255)               aaGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGAc                                                                                                                         <  1:279574/140‑1 (MQ=255)                aGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGAcc                                                                                                                        <  2:486516/140‑1 (MQ=255)                aGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGAcc                                                                                                                        <  1:867862/140‑1 (MQ=255)                aGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGAcc                                                                                                                        <  1:768572/140‑1 (MQ=255)                aGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGAcc                                                                                                                        <  1:49716/140‑1 (MQ=255)                 gTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCg                                                                                                                       >  2:1120466/1‑140 (MQ=255)                    ttCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGCCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGAt                                                                                                                    >  2:514903/1‑140 (MQ=255)                      cAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCa                                                                                                                  >  2:979231/1‑140 (MQ=255)                                        aGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTGCCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGa                                                                                                <  2:267606/140‑1 (MQ=255)                                        aGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGa                                                                                                <  2:615873/140‑1 (MQ=255)                                                      cGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCAt                                                                                  <  2:73324/140‑1 (MQ=255)                                                          tCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGt                                                                              >  2:1135003/1‑140 (MQ=255)                                                                gtcgtGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTcaccac                                                                        <  1:689647/140‑1 (MQ=255)                                                                      ttGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTc                                                                  >  2:394090/1‑140 (MQ=255)                                                                           agccaCATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGtt                                                             >  1:68803/1‑140 (MQ=255)                                                                                  ttGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGc                                                      <  2:1001048/140‑1 (MQ=255)                                                                                   tGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCt                                                     >  1:476261/1‑140 (MQ=255)                                                                                   tGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCt                                                     >  1:1095153/1‑140 (MQ=255)                                                                                        aaaTGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGc                                                      <  1:883638/134‑1 (MQ=255)                                                                                        aaaTGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGc                                                      >  2:883638/1‑134 (MQ=255)                                                                                             aaaCCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAAc                                           >  1:73128/1‑140 (MQ=255)                                                                                                      aGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGa                                  >  2:337038/1‑140 (MQ=255)                                                                                                            aTCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATgcg                            <  1:518549/140‑1 (MQ=255)                                                                                                              cAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAg                                         >  2:779532/1‑125 (MQ=255)                                                                                                              cAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAg                                         <  1:779532/125‑1 (MQ=255)                                                                                                                 gCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGcgccg                       >  2:111209/1‑140 (MQ=255)                                                                                                                 gCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGcgccg                       >  2:607601/1‑140 (MQ=255)                                                                                                                  ccGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGcgccgc                      >  1:157155/1‑140 (MQ=255)                                                                                                                  ccGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGcgccgc                      >  2:106099/1‑140 (MQ=255)                                                                                                                  ccGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGcgccg                       <  1:1138110/139‑1 (MQ=255)                                                                                                                  ccGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGcgccg                       >  2:1138110/1‑139 (MQ=255)                                                                                                                     tACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAgg                   >  2:696477/1‑140 (MQ=255)                                                                                                                      aCCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGt                  >  2:760279/1‑140 (MQ=255)                                                                                                                            gAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAgg            <  1:337038/140‑1 (MQ=255)                                                                                                                               gcgcCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAgg            >  1:642464/1‑137 (MQ=255)                                                                                                                               gcgcCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAgg            <  2:642464/137‑1 (MQ=255)                                                                                                                               gcgcCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGgcgc         >  1:725742/1‑140 (MQ=255)                                                                                                                                  ccACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTcc      >  1:907367/1‑140 (MQ=255)                                                                                                                                   cACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCa     <  1:607601/140‑1 (MQ=255)                                                                                                                                    aCGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCaa    >  2:499893/1‑140 (MQ=255)                                                                                                                                     cGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCaaa   >  2:451873/1‑140 (MQ=255)                                                                                                                                      gggCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAg  <  2:68803/140‑1 (MQ=255)                                                                                                                                      gggCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAg  <  2:73128/140‑1 (MQ=255)                                                                                                                                      gggCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAg  <  2:157155/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGTCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAG  >  NC_000913/2965845‑2966117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑