Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,976,831	T→C	V78A (GTG→GCG)	galR →	DNA‑binding transcriptional dual regulator GalR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,976,831	0	T	C	100.0%	185.9 / NA	51	V78A (GTG→GCG)	galR	DNA‑binding transcriptional dual regulator GalR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

CTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCC  >  NC_000913/2976698‑2976967                                                                                                                                      |                                                                                                                                         ctggctgTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGc                                                                                                                                    <  1:354342/140‑1 (MQ=255)   ggctgTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCgg                                                                                                                                  <  2:723133/140‑1 (MQ=255)    gctgTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGt                                                                                                                                 <  2:418145/140‑1 (MQ=255)              gTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTg                                                                                                                       <  1:328204/140‑1 (MQ=255)              gTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTg                                                                                                                       <  1:429612/140‑1 (MQ=255)               tGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTgg                                                                                                                      <  1:746720/140‑1 (MQ=255)                  aaTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGt                                                                                                                                 >  2:31709/1‑126 (MQ=255)                  aaTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGt                                                                                                                                 <  1:31709/126‑1 (MQ=255)                  aaTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCtt                                                                                                                   >  1:398802/1‑140 (MQ=255)                   aTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGTAACGGTCGGTCTGGTCGGTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTa                                                                                                                  >  2:1039272/1‑140 (MQ=255)                         tctcTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCacac                                                                                                            >  2:639493/1‑140 (MQ=255)                         tctcTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCacac                                                                                                            >  1:840092/1‑140 (MQ=255)                         tctcTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCacac                                                                                                            >  1:533407/1‑140 (MQ=255)                              tAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTgg                                                                                                                      >  1:443741/1‑125 (MQ=255)                              tAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTgg                                                                                                                      <  2:443741/125‑1 (MQ=255)                              tAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTa                                                                                                       >  1:246182/1‑140 (MQ=255)                                gCTATCACCCGAACGCCAACGCCCGTGTGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAt                                                                                                     >  2:47496/1‑140 (MQ=255)                                     cACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAtttttt                                                                                                >  1:494007/1‑140 (MQ=255)                                     cACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAtttttt                                                                                                >  1:654436/1‑140 (MQ=255)                                     cACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAtttttt                                                                                                >  2:1011634/1‑140 (MQ=255)                                      aCCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTa                                                                                               <  2:654436/140‑1 (MQ=255)                                      aCCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTa                                                                                               <  2:676138/140‑1 (MQ=255)                                       cccGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTGGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTat                                                                                              >  1:171134/1‑140 (MQ=255)                                       cccGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTat                                                                                              >  1:916536/1‑140 (MQ=255)                                       cccGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTat                                                                                              >  2:1129353/1‑140 (MQ=255)                                         cGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTattg                                                                                            <  1:639493/140‑1 (MQ=255)                                          gAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTattga                                                                                           <  1:635495/140‑1 (MQ=255)                                           aacgccaacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTattgat                                                                                          >  1:1089845/1‑140 (MQ=255)                                               ccaacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGc                                                                                      <  2:1089845/140‑1 (MQ=255)                                                caacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCa                                                                                     <  1:1011634/140‑1 (MQ=255)                                                caacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCa                                                                                     <  2:840092/140‑1 (MQ=255)                                                caacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCa                                                                                     >  1:72388/1‑140 (MQ=255)                                                  acgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTGGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAAc                                                                                   <  2:171134/140‑1 (MQ=255)                                                    gccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACgg                                                                                 <  2:494007/140‑1 (MQ=255)                                                                gcgcAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGa                                                                     >  1:282611/1‑140 (MQ=255)                                                                        gACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAag                                                             <  2:72388/140‑1 (MQ=255)                                                                          ccACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAagag                                                           <  2:398802/140‑1 (MQ=255)                                                                          ccACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAagag                                                           <  2:533407/140‑1 (MQ=255)                                                                                aaaCGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAg                                                     >  2:772017/1‑140 (MQ=255)                                                                                     gtcggtcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCAt                                                >  2:947321/1‑140 (MQ=255)                                                                                     gtcggtcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCAt                                                >  2:602600/1‑140 (MQ=255)                                                                                          tcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGc                                           >  2:864697/1‑140 (MQ=255)                                                                                                    ggTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATAGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCg                                 >  2:630213/1‑140 (MQ=255)                                                                                                             tCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCtg                        >  2:712939/1‑140 (MQ=255)                                                                                                              ccGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCtgt                       <  1:1014396/140‑1 (MQ=255)                                                                                                                gATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGtgc                     <  1:772017/140‑1 (MQ=255)                                                                                                                        tttCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATCGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTgg             >  2:141706/1‑140 (MQ=255)                                                                                                                        tttCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATCGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTgg             <  1:141706/140‑1 (MQ=255)                                                                                                                             gTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGgtcgtc        <  2:916536/140‑1 (MQ=255)                                                                                                                              tGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTcc       >  1:85451/1‑140 (MQ=255)                                                                                                                              tGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTcc       >  1:113801/1‑140 (MQ=255)                                                                                                                                   tGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGcc  <  2:85451/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCC  >  NC_000913/2976698‑2976967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑