| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,137,213 | C→T | C437Y (TGC→TAC) | gss ← | fused glutathionylspermidine amidase/glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,137,213 | 0 | C | T | 98.4% | 217.6 / ‑4.2 | 64 | C437Y (TGC→TAC) | gss | fused glutathionylspermidine amidase/glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (28/35); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
GGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCATAAACTGCGCGTGATAG > NC_000913/3137078‑3137344 | gggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGtt < 1:161856/140‑1 (MQ=255) cgcAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAAc > 1:924515/1‑140 (MQ=255) aaTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCg < 1:898454/140‑1 (MQ=255) aaTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCg < 2:1052620/140‑1 (MQ=255) aaTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCg < 1:331699/140‑1 (MQ=255) aCGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTACCCAGTCGccc < 2:132880/140‑1 (MQ=255) cGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAg < 2:693363/130‑1 (MQ=255) cGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAg > 1:693363/1‑130 (MQ=255) cGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTcc < 2:382845/140‑1 (MQ=255) aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTcccc > 2:908684/1‑140 (MQ=255) aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTcccc > 1:999314/1‑140 (MQ=255) tAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCa < 2:383090/140‑1 (MQ=255) aaGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCat < 1:258740/140‑1 (MQ=255) cACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCatcaatca > 1:290776/1‑140 (MQ=255) cTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTATCTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGt > 1:423457/1‑140 (MQ=255) ttttGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGccc < 2:638799/140‑1 (MQ=255) gCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgca > 1:706517/1‑140 (MQ=255) cGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTATCTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgcag < 2:423457/140‑1 (MQ=255) cGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgcag < 1:908684/140‑1 (MQ=255) cGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgcag < 2:924515/140‑1 (MQ=255) gACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcc < 1:734192/140‑1 (MQ=255) gACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcc > 1:901086/1‑140 (MQ=255) aCCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATccc > 1:451977/1‑140 (MQ=255) ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagc < 2:352751/140‑1 (MQ=255) ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagc < 2:901086/140‑1 (MQ=255) ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagc < 1:1088722/140‑1 (MQ=255) gTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagcc > 1:241488/1‑140 (MQ=255) cGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagcccag < 2:51707/140‑1 (MQ=255) ttGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCa > 1:382381/1‑140 (MQ=255) cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagc > 1:1026319/1‑128 (MQ=255) cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagc < 2:1026319/128‑1 (MQ=255) cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcca > 1:417811/1‑140 (MQ=255) cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcca > 2:629089/1‑140 (MQ=255) cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcca < 2:621848/140‑1 (MQ=255) ccGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaat < 2:706517/140‑1 (MQ=255) ccGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaat < 2:382381/140‑1 (MQ=255) ccGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaat < 2:373133/140‑1 (MQ=255) gcagcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaatcc > 2:332716/1‑140 (MQ=255) cagcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaatcca > 2:649790/1‑140 (MQ=255) gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACg < 1:629089/140‑1 (MQ=255) gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACg < 2:451977/140‑1 (MQ=255) gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACg < 2:290776/140‑1 (MQ=255) gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCACCCAATCCACg < 1:671059/140‑1 (MQ=255) gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTACCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACg < 1:222414/140‑1 (MQ=255) gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACg < 1:553681/140‑1 (MQ=255) cGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATAcg < 2:241488/140‑1 (MQ=255) ggTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATAcgc < 2:417811/140‑1 (MQ=255) cACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGccc > 1:749505/1‑140 (MQ=255) cACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGccc > 2:42154/1‑140 (MQ=255) cACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGccc > 2:630007/1‑140 (MQ=255) cGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGc > 1:429875/1‑140 (MQ=255) cGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGc > 2:1050772/1‑140 (MQ=255) tGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTg > 2:715935/1‑140 (MQ=255) tGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTg > 1:962319/1‑140 (MQ=255) tGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTg > 2:126124/1‑140 (MQ=255) gATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGc < 2:108415/140‑1 (MQ=255) aaCGCGGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAgcgc < 1:630007/140‑1 (MQ=255) cgcgGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCt < 2:749505/140‑1 (MQ=255) cgcgGTTTCCCACGCCCAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCt < 2:101667/140‑1 (MQ=255) cccAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCATAAACTgc < 1:649790/140‑1 (MQ=255) ccAGGTTTTCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCATAAACTgcg > 2:1016016/1‑140 (MQ=255) tCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCATAAACTGCGCGTGATAg > 1:341373/1‑140 (MQ=255) tCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCATAAACTGCGCGTGATAg > 2:540659/1‑140 (MQ=255) tCCACACGTAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCATAAACTGCGCGTGATAg > 1:1097123/1‑140 (MQ=255) | GGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCATAAACTGCGCGTGATAG > NC_000913/3137078‑3137344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |