Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,242,404	(A)5→4	intergenic (‑150/+222)	xylE ← / ← malG	D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,242,400	0	A	.	100.0%	182.1 / NA	43	intergenic (‑146/+226)	xylE/malG	D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/21);  total (22/21)

CCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGATAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAA  >  NC_000913/4242264‑4242529                                                                                                                                         |                                                                                                                                  ccTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGATaaaa                                                                                                                                <  1:436509/140‑1 (MQ=255)          aTTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTAt                                                                                                                      <  1:538983/140‑1 (MQ=255)           ttCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGATaaaacaa                                                                                                                             <  1:931921/133‑4 (MQ=255)           ttCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGATaaaacaa                                                                                                                             >  2:931921/1‑130 (MQ=255)           ttCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTAtt                                                                                                                     >  2:766864/1‑140 (MQ=255)           ttCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTAtt                                                                                                                     >  2:731743/1‑140 (MQ=255)           ttCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTAtt                                                                                                                     >  2:60397/1‑140 (MQ=255)           ttCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTAtt                                                                                                                     >  2:395410/1‑140 (MQ=255)              tGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCCGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGcc                                                                                                                  <  1:1028045/140‑1 (MQ=255)                    tttATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACt                                                                                                            <  1:255893/140‑1 (MQ=255)                    tttATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACt                                                                                                            <  2:725269/140‑1 (MQ=255)                    tttATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACt                                                                                                            <  1:60397/140‑1 (MQ=255)                        tCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCaga                                                                                                        >  1:56316/1‑140 (MQ=255)                            tGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCAc                                                                                                    >  1:921978/1‑140 (MQ=255)                                 tGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAag                                                                                               >  2:157000/1‑140 (MQ=255)                                      ttGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAAt                                                                                          >  1:310410/1‑140 (MQ=255)                                      ttGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAAt                                                                                          >  1:534516/1‑140 (MQ=255)                                        ggAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTa                                                                                        >  2:315648/1‑140 (MQ=255)                                              gTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGa                                                                                                  <  2:591886/124‑1 (MQ=255)                                              gTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGa                                                                                                  >  1:591886/1‑124 (MQ=255)                                              gTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTaa                                                                                  >  2:464785/1‑140 (MQ=255)                                                    cAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGa                                                                            <  1:579335/140‑1 (MQ=255)                                                       ttAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGaaaa                                                                         >  1:1057883/1‑140 (MQ=255)                                                                 tATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGt                                                               >  2:756391/1‑140 (MQ=255)                                                                          ttACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAaataat                                                      <  1:157000/140‑1 (MQ=255)                                                                          ttACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAaataat                                                      <  2:785836/140‑1 (MQ=255)                                                                             cAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAg                                                   <  1:464785/140‑1 (MQ=255)                                                                               aTGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGct                                                 <  1:145785/140‑1 (MQ=255)                                                                                 gCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGctct                                               >  2:679603/1‑140 (MQ=255)                                                                                     ttACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTatga                                           <  1:766864/140‑1 (MQ=255)                                                                                        cGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATgg                                        <  1:33820/140‑1 (MQ=255)                                                                                        cGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATgg                                        <  2:1057883/140‑1 (MQ=255)                                                                                                        tgATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGa                        <  2:534516/140‑1 (MQ=255)                                                                                                         gATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGaa                       <  2:310410/140‑1 (MQ=255)                                                                                                         gATCTTAATTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGaa                       <  1:961288/140‑1 (MQ=255)                                                                                                               aaTTGTGATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTc                 >  2:897245/1‑140 (MQ=255)                                                                                                                  tgtgATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGa              <  1:1094343/140‑1 (MQ=255)                                                                                                                    tgATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGaa                       <  1:137116/129‑1 (MQ=255)                                                                                                                    tgATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGaa                       >  2:137116/1‑129 (MQ=255)                                                                                                                    tgATAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGAtt            >  1:548883/1‑140 (MQ=255)                                                                                                                      aTAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGc          >  1:665473/1‑140 (MQ=255)                                                                                                                      aTAATTATCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGc          >  1:311871/1‑140 (MQ=255)                                                                                                                      aTAATTATACAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGc          >  1:21623/1‑140 (MQ=255)                                                                                                                             tCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACgaa   <  2:311871/140‑1 (MQ=255)                                                                                                                             tCCAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACgaa   <  2:972585/140‑1 (MQ=255)                                                                                                                              ccAAAATTGAT‑AAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACgaaa  >  1:175997/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  CCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCAAAATTGATAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAA  >  NC_000913/4242264‑4242529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑