| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,740,439 | Δ1 bp | coding (712/1071 nt) | aroF ← | 3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,740,437 | 0 | T | . | 98.3% | 241.3 / ‑1.9 | 59 | coding (714/1071 nt) | aroF | 3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (27/31); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
TGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGA > NC_000913/2740302‑2740572 | tGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttacc > 2:922308/1‑137 (MQ=255)tGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttacc > 2:64350/1‑137 (MQ=255) gCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGGGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttacca < 2:896685/140‑5 (MQ=255) ccACCGCAGGCTGACGCCGATAATCTTTATTGAAATTACCGTGGCTGCAATCTACCATCAGAGACGGACGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTaccac > 2:589426/1‑140 (MQ=255) cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACcgcg < 1:922308/140‑1 (MQ=255) cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACcgcg < 2:130072/140‑1 (MQ=255) cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACcgcg > 1:331534/1‑140 (MQ=255) cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACcgcg < 1:385875/140‑1 (MQ=255) aaTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGt < 1:1091264/140‑1 (MQ=255) tGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTcc > 2:728902/1‑140 (MQ=255) aTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTccctga > 1:476427/1‑137 (MQ=255) aTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTg > 2:200586/1‑140 (MQ=255) cGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCt < 1:974237/134‑1 (MQ=255) cGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGGTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCt > 2:974237/1‑134 (MQ=255) ggCTGCAATCTCCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTa < 1:589426/140‑1 (MQ=255) gCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAg > 1:172362/1‑140 (MQ=255) gCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTACGGATTCCCCTGAGTTTGTAg > 2:549824/1‑140 (MQ=255) cattCTTCCACCAGCGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaac < 1:563583/137‑1 (MQ=255) aCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcc > 2:417411/1‑140 (MQ=255) cATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctg < 1:64350/140‑1 (MQ=255) aTCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgc < 2:547228/140‑1 (MQ=255) aTCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATT‑CCCTGAGTTTGTAGCAACGCAAcctgcc < 2:476427/140‑1 (MQ=255) tCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc < 1:260350/140‑1 (MQ=255) tCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc < 2:172362/140‑1 (MQ=255) tCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc < 2:289179/140‑1 (MQ=255) tCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTACGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc < 1:549824/140‑1 (MQ=255) tCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGCCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc < 2:994545/140‑1 (MQ=255) gagaCGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc > 1:827054/1‑137 (MQ=255) gagaCGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc < 2:827054/137‑1 (MQ=255) agaCGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctgc > 1:716132/1‑140 (MQ=255) cGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaac > 1:185057/1‑123 (MQ=255) cGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaac < 2:185057/123‑1 (MQ=255) cGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcctg > 1:478250/1‑140 (MQ=255) gggCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctgc > 1:472169/1‑136 (MQ=255) gggCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctgc < 2:472169/136‑1 (MQ=255) gcgcAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc < 1:413431/130‑1 (MQ=255) gcgcAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc > 2:413431/1‑130 (MQ=255) gTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGc < 2:716132/140‑1 (MQ=255) ccGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACctgccctgcc > 1:533408/1‑127 (MQ=255) ccGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACctgccctgcc < 2:533408/127‑1 (MQ=255) ccGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaa < 2:478250/140‑1 (MQ=255) tGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa < 1:728902/140‑1 (MQ=255) tCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg > 2:579626/1‑140 (MQ=255) tCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg > 1:1073445/1‑140 (MQ=255) tCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg > 2:18615/1‑140 (MQ=255) tCCATCTCTTTTTCACATTGAGCAACATCCGTAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGCCTCACATGGCCGTCCGGATTCCCCTGAGCTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg > 2:766393/1‑140 (MQ=255) tttCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTggg < 2:681826/140‑1 (MQ=255) aTTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcggcg > 1:228820/1‑140 (MQ=255) aTTGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcggctg > 1:930534/1‑138 (MQ=255) tGCGCAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGgcggcggcg > 1:575734/1‑140 (MQ=255) cgcAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcggc > 1:913575/1‑134 (MQ=255) cgcAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcggc < 2:913575/134‑1 (MQ=255) cgcAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGgcgcg > 1:699780/1‑140 (MQ=255) cgcAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGgcgcg > 2:917714/1‑140 (MQ=255) cgcAACATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGgcgcg > 1:741259/1‑140 (MQ=255) aaCATCCGCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCAt < 1:740303/140‑1 (MQ=255) gCAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTa < 1:417411/140‑1 (MQ=255) cAGGGCTATAGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTaa < 1:200586/140‑1 (MQ=255) ataGTTCGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGt < 1:485681/140‑1 (MQ=255) cGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAg > 1:437406/1‑140 (MQ=255) cGGCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAg < 2:930534/140‑1 (MQ=255) ggCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGa < 1:579626/140‑1 (MQ=255) ggCGC‑TTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGa < 2:575734/140‑1 (MQ=255) | TGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGA > NC_000913/2740302‑2740572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |