Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,897,937	Δ1 bp	intergenic (‑67/+27)	ygcS ← / ← ygcU	putative transporter YgcS/putative FAD‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,897,934	0	C	.	100.0%	146.5 / NA	35	intergenic (‑64/+30)	ygcS/ygcU	putative transporter YgcS/putative FAD‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (18/17);  total (18/17)

AAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGCCGTGTTC  >  NC_000913/2897798‑2898062                                                                                                                                         |                                                                                                                                 aaGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGcccg                                                                                                                               <  2:902557/140‑2 (MQ=255)                     cAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTg                                                                                                          >  1:871298/1‑140 (MQ=255)                       gTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGac                                                                                                        >  1:1040563/1‑140 (MQ=255)                                 ttAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTAtttt                                                                                              >  2:18638/1‑140 (MQ=255)                                    aGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCa                                                                                           <  1:986008/140‑1 (MQ=255)                                      cGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAAt                                                                                         <  1:950763/140‑1 (MQ=255)                                          aaaTCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGga                                                                                     <  1:18638/140‑1 (MQ=255)                                          aaaTCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGga                                                                                     <  2:871298/140‑1 (MQ=255)                                           aaTCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGgat                                                                                    <  1:1082734/140‑1 (MQ=255)                                                   aTTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTa                                                                            <  2:930132/140‑1 (MQ=255)                                                      cGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTAccc                                                                         <  1:317836/140‑1 (MQ=255)                                                           cGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTAtt                                                                    >  2:785936/1‑140 (MQ=255)                                                              tGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCAt                                                                 <  1:785936/140‑1 (MQ=255)                                                               gAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATa                                                                <  2:1040563/140‑1 (MQ=255)                                                                   tgtTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGc                                                            >  1:309037/1‑140 (MQ=255)                                                                     ttCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCa                                                          >  2:120998/1‑140 (MQ=255)                                                                        aTCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTa                                                       <  2:896055/140‑1 (MQ=255)                                                                                  cTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACt                                             <  2:309037/140‑1 (MQ=255)                                                                                    tCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGc                                           >  2:279260/1‑140 (MQ=255)                                                                                          tCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTc                                     >  2:273822/1‑140 (MQ=255)                                                                                          tCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTc                                     >  1:74622/1‑140 (MQ=255)                                                                                               taataaGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGccc                                >  2:742220/1‑140 (MQ=255)                                                                                                    aGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCa                           >  1:519693/1‑140 (MQ=255)                                                                                                      aaCGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGc                                  >  2:309017/1‑131 (MQ=255)                                                                                                      aaCGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGc                                  <  1:309017/131‑1 (MQ=255)                                                                                                      aaCGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGc                         <  1:715381/140‑1 (MQ=255)                                                                                                        cGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCaa                       >  1:114216/1‑140 (MQ=255)                                                                                                         ggCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGc                                  <  1:571865/128‑1 (MQ=255)                                                                                                         ggCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGc                                  >  2:571865/1‑128 (MQ=255)                                                                                                         ggCTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAAc                      >  2:107329/1‑140 (MQ=255)                                                                                                           cTTCTCGCCTGAGAAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGc                    >  2:733982/1‑140 (MQ=255)                                                                                                                     gagaAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCAcgcg             <  1:734626/137‑1 (MQ=255)                                                                                                                     gagaAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCAcgcg             >  2:734626/1‑137 (MQ=255)                                                                                                                       gaAGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGcc        <  1:733982/140‑1 (MQ=255)                                                                                                                         aGCCGAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGCCgt      >  2:610071/1‑140 (MQ=255)                                                                                                                             gAAAAGGCAACG‑CCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGCCGTGTTc  <  1:107329/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 AAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGCCGTGTTC  >  NC_000913/2897798‑2898062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑