| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,999,680 | (G)7→8 | coding (212/756 nt) | ygeR ← | LysM domain‑containing putative peptidase lipoprotein YgeR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,999,673 | 1 | . | G | 94.1% | 171.7 / 3.5 | 51 | coding (219/756 nt) | ygeR | LysM domain‑containing putative peptidase lipoprotein YgeR |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (20/28); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTG > NC_000913/2999536‑2999791 | ggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑gg > 2:995066/1‑140 (MQ=255) cAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCACCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑ggggg > 1:317471/1‑140 (MQ=255) ggATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑ggggg‑gg > 1:377804/1‑140 (MQ=255) ggATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑ggggg‑gg > 2:373478/1‑140 (MQ=255) gTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGtt > 2:625764/1‑140 (MQ=255) cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTcg > 1:878354/1‑140 (MQ=255) cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTcg < 2:446436/140‑1 (MQ=255) tgatgaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTcgc < 1:625764/140‑1 (MQ=255) atgaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCgcgc < 1:715282/140‑1 (MQ=255) ggTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGtt > 2:10263/1‑140 (MQ=255) aaCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCtttt > 1:810476/1‑140 (MQ=255) aaCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCtttt < 2:135858/140‑1 (MQ=255) aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt < 1:428210/140‑1 (MQ=255) aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt < 2:878354/140‑1 (MQ=255) gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg > 2:16080/1‑140 (MQ=255) gTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTc < 2:617435/140‑1 (MQ=255) ttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCg < 1:474571/140‑1 (MQ=255) tGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AGGGGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTGCGCTGGTTCCCg < 1:294300/140‑1 (MQ=255) gTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AGGGGGGG‑GGAAAGCCCGTTCA‑TCGCGCCCGGTCTTTTTAGCTTGGTTCCgtgg > 1:928074/1‑140 (MQ=255) ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCACCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg < 2:317471/140‑1 (MQ=255) gttttGGGGGTTGATTTACGTGTAATACTGTTCCTTTTCGCCCCCCCAATTTTTAGTTTCTGCCAAACTCAAATGGTGTAGG‑‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 2:928074/138‑1 (MQ=255) gATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 1:995066/140‑1 (MQ=255) tttGGCGGTTGATTTACGTGTAATACTGCTAC‑TTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaaa < 2:15197/140‑1 (MQ=255) tGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAAt < 1:671597/140‑1 (MQ=255) ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa < 1:10263/140‑1 (MQ=255) ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa > 2:718443/1‑140 (MQ=255) aCGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTa < 1:718443/140‑1 (MQ=255) aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccccc > 2:1029515/1‑140 (MQ=255) tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTACCCCCCCGttt > 1:494061/1‑140 (MQ=255) gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc > 2:10000/1‑140 (MQ=255) gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc > 2:842734/1‑140 (MQ=255) gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc < 2:448875/140‑1 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcc > 1:381214/1‑130 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcc < 2:381214/130‑1 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa < 1:936285/140‑1 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa > 1:76655/1‑140 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa > 2:602808/1‑140 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa > 2:413493/1‑140 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑GGGGGTGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCa > 1:179684/1‑140 (MQ=255) tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc < 1:602808/140‑1 (MQ=255) aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACg > 1:73379/1‑140 (MQ=255) tttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGgtg < 1:373478/140‑1 (MQ=255) cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc < 2:840125/133‑1 (MQ=255) cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc > 1:840125/1‑133 (MQ=255) ccccACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAAc > 1:230557/1‑140 (MQ=255) acccaGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAg < 1:71104/140‑1 (MQ=255) caGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTACCCCCCCGTTTCACGGTGTAAACGGAGCCg < 2:494061/140‑1 (MQ=255) aGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCgg > 2:333678/1‑140 (MQ=255) ttttAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑GGGGGTGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa < 2:179684/140‑1 (MQ=255) ttAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 2:377804/140‑1 (MQ=255) ttAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 1:513847/140‑1 (MQ=255) ttAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 1:333678/140‑1 (MQ=255) ttAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 2:85563/140‑1 (MQ=255) ttAGTTTCTGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 1:312894/140‑1 (MQ=255) tGACCAACTTCAATGGTGTA‑AG‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTg > 2:154571/1‑140 (MQ=255) | GGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTA‑A‑‑GGGGG‑GGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTG > NC_000913/2999536‑2999791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |