Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,863,987	(A)7→8	coding (89/717 nt)	yidP →	putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,863,980	1	.	A	100.0%	288.1 / NA	80	E28K (GAA→AAA)	yidP	putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (39/41);  total (39/41)

ACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTG‑AAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACT  >  NC_000913/3863851‑3864110                                                                                                                                   |                                                                                                                                   aCTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTG‑aaaaaaaagc                                                                                                                          >  2:167322/1‑137 (MQ=255) aCTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTG‑aaaaaaaagc                                                                                                                          >  2:1083719/1‑137 (MQ=255)  cTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTG‑aaaaaaaagct                                                                                                                         >  1:1053776/1‑136 (MQ=255)  cTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTG‑aaaaaaaagct                                                                                                                         >  2:92536/1‑136 (MQ=255)     gtgtgCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGc                                                                                                                       >  2:899301/1‑140 (MQ=255)          cgcATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAag                                                                                                                  >  2:303494/1‑140 (MQ=255)           gcATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAaga                                                                                                                 <  1:1082413/140‑1 (MQ=255)               tACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGttt                                                                                                             >  2:723707/1‑140 (MQ=255)                  ccGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCg                                                                                                          <  1:1083719/140‑1 (MQ=255)                  ccGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCg                                                                                                          <  1:723707/140‑1 (MQ=255)                     ggCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGa                                                                                                                    <  2:1020100/127‑1 (MQ=255)                     ggCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGa                                                                                                                    >  1:1020100/1‑127 (MQ=255)                     ggCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTa                                                                                                       <  2:937571/140‑1 (MQ=255)                     ggCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTa                                                                                                       >  2:788396/1‑140 (MQ=255)                     ggCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTACGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTa                                                                                                       >  2:45663/1‑140 (MQ=255)                       cTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAaga                                                                                                                 >  2:484816/1‑128 (MQ=255)                       cTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAaga                                                                                                                 <  1:484816/128‑1 (MQ=255)                       cTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATc                                                                                                     >  2:517318/1‑140 (MQ=255)                       cTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATc                                                                                                     <  1:99387/140‑1 (MQ=255)                       cTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATc                                                                                                     >  1:613491/1‑140 (MQ=255)                         tGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATcgc                                                                                                   >  1:480778/1‑140 (MQ=255)                           cAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCgg                                                                                                 >  1:659978/1‑140 (MQ=255)                                 aaaaaGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGAcc                                                                                           <  2:613491/140‑1 (MQ=255)                                 aaaaaGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGAcc                                                                                           <  2:659978/140‑1 (MQ=255)                                  aaaaGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCa                                                                                          <  1:92536/140‑1 (MQ=255)                                        gaTCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATg                                                                                              <  2:414150/130‑1 (MQ=255)                                        gaTCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATg                                                                                              >  1:414150/1‑130 (MQ=255)                                        gaTCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTa                                                                                    <  2:124377/140‑1 (MQ=255)                                        gaTCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTa                                                                                    <  1:517318/140‑1 (MQ=255)                                         aTCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTaa                                                                                   <  1:145925/140‑1 (MQ=255)                                         aTCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTaa                                                                                   <  2:480778/140‑1 (MQ=255)                                                 atgatCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTg                                                                           >  1:70720/1‑140 (MQ=255)                                                 atgatCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTg                                                                           >  1:577450/1‑140 (MQ=255)                                                   gatCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGAc                                                                         >  1:916872/1‑140 (MQ=255)                                                    atCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGAcc                                                                        >  2:170866/1‑140 (MQ=255)                                                    atCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGAcc                                                                        >  1:638516/1‑140 (MQ=255)                                                    atCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGAcc                                                                        >  1:54651/1‑140 (MQ=255)                                                    atCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGAcc                                                                        >  2:936083/1‑140 (MQ=255)                                                    atCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGAcc                                                                        <  2:70720/140‑1 (MQ=255)                                                    atCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGAcc                                                                        >  2:789613/1‑140 (MQ=255)                                                     tCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACct                                                                       >  1:819748/1‑140 (MQ=255)                                                     tCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACct                                                                       >  2:208446/1‑140 (MQ=255)                                                        aCAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACctgct                                                                    >  1:634730/1‑140 (MQ=255)                                                              gCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGc                                                              >  1:831250/1‑140 (MQ=255)                                                              gCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGc                                                              >  2:246164/1‑140 (MQ=255)                                                              gCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGc                                                              <  1:303494/140‑1 (MQ=255)                                                                aTTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGt                                                            >  2:363018/1‑140 (MQ=255)                                                                aTTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGt                                                            >  2:617403/1‑140 (MQ=255)                                                                 ttacGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTg                                                           <  1:45663/137‑1 (MQ=255)                                                                 ttGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTg                                                           <  2:569499/140‑1 (MQ=255)                                                                 ttGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTg                                                           >  2:875932/1‑140 (MQ=255)                                                                 ttGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTg                                                           <  1:788396/140‑1 (MQ=255)                                                                  tGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTgg                                                          >  2:25840/1‑140 (MQ=255)                                                                   gCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTggg                                                         >  2:16131/1‑140 (MQ=255)                                                                    cGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTgggg                                                        <  2:1053776/140‑1 (MQ=255)                                                                     ggAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTggggg                                                       <  1:936083/140‑1 (MQ=255)                                                                          ggTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCtggt                                                  <  2:577450/140‑1 (MQ=255)                                                                              aaGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTc                                              >  2:668688/1‑140 (MQ=255)                                                                               aGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTcc                                             <  1:25840/140‑1 (MQ=255)                                                                                   ttCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTccgccg                                         <  1:789613/140‑1 (MQ=255)                                                                                    tCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCACCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCcgccgc                                        <  2:35747/140‑1 (MQ=255)                                                                                      gACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCa                                      <  1:167322/140‑1 (MQ=255)                                                                                      gACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCa                                      <  1:363018/140‑1 (MQ=255)                                                                                      gACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCa                                      <  1:176517/140‑1 (MQ=255)                                                                                       aCTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCAc                                     <  1:170866/140‑1 (MQ=255)                                                                                            aCTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAg                                <  2:207234/140‑1 (MQ=255)                                                                                            aCTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAg                                <  2:54651/140‑1 (MQ=255)                                                                                            aCTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAg                                <  2:831250/140‑1 (MQ=255)                                                                                            aCTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAg                                <  2:638516/140‑1 (MQ=255)                                                                                              tCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTg                              <  1:875932/140‑1 (MQ=255)                                                                                               ccGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTgg                             <  1:246164/140‑1 (MQ=255)                                                                                                 gCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCa                           >  1:777251/1‑140 (MQ=255)                                                                                                 gCAGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCa                           >  1:279540/1‑140 (MQ=255)                                                                                                   aGATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACt                         >  2:58942/1‑140 (MQ=255)                                                                                                       ttCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTAcc                     >  1:134894/1‑140 (MQ=255)                                                                                                           cGCTAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGt                 <  1:899301/140‑1 (MQ=255)                                                                                                              tAAACAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTgcg              <  2:777251/140‑1 (MQ=255)                                                                                                                 aCAGCCTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCaa           >  1:211992/1‑140 (MQ=255)                                                                                                                     ccTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTg                   >  2:1087140/1‑128 (MQ=255)                                                                                                                     ccTTCTTCCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTg                   <  1:1087140/128‑1 (MQ=255)                                                                                                                        tcttcCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTAc                      <  1:180689/122‑1 (MQ=255)                                                                                                                        tcttcCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTAc                      >  2:180689/1‑122 (MQ=255)                                                                                                                        tcttcCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTa    <  2:916872/140‑1 (MQ=255)                                                                                                                          ttcCCGGTGAAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACt  >  1:954014/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   ACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTTAACTCCGCAGATTTCACGCTAAACAGCCTTCTTCCCGGTG‑AAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACT  >  NC_000913/3863851‑3864110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑