Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→9	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,086	1	.	G	84.0%	146.9 / 21.9	50	noncoding (80/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (3/5);  new base G (24/18);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTT  >  NC_000913/4605953‑4606219                                                                                                                                       |                                                                                                                                        aCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑gggggg                                                                                                                                  <  1:882542/140‑1 (MQ=255)  cAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggggggg                                                                                                                                 <  2:694531/140‑1 (MQ=255)   aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggggggg‑g                                                                                                                               >  1:939088/1‑140 (MQ=255)        cGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggggggg‑ggactt                                                                                                                          <  2:489519/140‑6 (MQ=255)          gCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑ggggggg‑gggacttg                                                                                                                        <  2:939088/140‑8 (MQ=255)                cATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCa                                                                                                                  <  2:154755/140‑1 (MQ=255)                 aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCa                                                                                                                  <  2:535649/140‑1 (MQ=255)                  ttttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCAc                                                                                                                 <  1:161542/140‑1 (MQ=255)                     tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAAccccc                                                                                                                   <  2:982737/135‑1 (MQ=255)                     tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAAccccc                                                                                                                   >  1:982737/1‑135 (MQ=255)                     tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCAc                                                                                                                 <  1:338777/137‑1 (MQ=255)                     tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCAc                                                                                                                 >  2:338777/1‑137 (MQ=255)                       cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCg                                                                                                            >  1:1007510/1‑140 (MQ=255)                       cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCg                                                                                                            >  1:107190/1‑140 (MQ=255)                          ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAAcccc                                                                                                                    >  2:894592/1‑129 (MQ=255)                          ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAAcccc                                                                                                                    <  1:894592/129‑1 (MQ=255)                                  ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAGGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                 >  2:295737/1‑140 (MQ=255)                                  ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                 >  2:876625/1‑140 (MQ=255)                                      gCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                             >  2:1038393/1‑140 (MQ=255)                                       cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                             >  1:643367/1‑140 (MQ=255)                                       cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                            <  1:797531/140‑1 (MQ=255)                                                cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                   <  1:197037/140‑1 (MQ=255)                                                cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACATTAACACCTGAAGCTAgc                                                                                  <  2:959805/140‑1 (MQ=255)                                                 tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                                                                  <  1:1038393/140‑1 (MQ=255)                                                  ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                <  1:1053022/125‑1 (MQ=255)                                                  ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                >  2:1053022/1‑125 (MQ=255)                                                  ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                 >  1:769440/1‑140 (MQ=255)                                                           cTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                        <  1:577513/140‑1 (MQ=255)                                                             gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                      >  1:217823/1‑140 (MQ=255)                                                             gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                                     >  1:162718/1‑140 (MQ=255)                                                                 atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                 <  2:162718/140‑1 (MQ=255)                                                                  tAATCAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGGTGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                 <  2:1007510/140‑1 (MQ=255)                                                                   aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                >  2:490187/1‑140 (MQ=255)                                                                   aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                >  1:347679/1‑140 (MQ=255)                                                                    aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                               >  2:1061979/1‑140 (MQ=255)                                                                        aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                            >  1:4251/1‑140 (MQ=255)                                                                        aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGGCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          >  2:173049/1‑140 (MQ=255)                                                                             aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                      <  1:713165/140‑1 (MQ=255)                                                                              aTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca                                                     <  1:607785/140‑1 (MQ=255)                                                                                       gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTa                                            <  2:769440/140‑1 (MQ=255)                                                                                             tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                       >  1:1000472/1‑123 (MQ=255)                                                                                             tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                       <  2:1000472/123‑1 (MQ=255)                                                                                             tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACttttt                                      >  2:633675/1‑140 (MQ=255)                                                                                                 ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                 >  1:185776/1‑140 (MQ=255)                                                                                                  gaagaaACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                                <  2:185776/140‑1 (MQ=255)                                                                                                     gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                              <  1:1061979/140‑1 (MQ=255)                                                                                                             aaaGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                      >  2:1067255/1‑140 (MQ=255)                                                                                                             aaaGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                      <  1:295737/140‑1 (MQ=255)                                                                                                               aGGAAGTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTg                    <  1:490187/140‑1 (MQ=255)                                                                                                                    gTAAAGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTATCGCGTCTACCAACTCCGCCACCTTCGCACAGACATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg               >  2:1018071/1‑140 (MQ=21)                                                                                                                       aaGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg            <  1:466792/140‑1 (MQ=255)                                                                                                                       aaGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg            >  2:564126/1‑140 (MQ=255)                                                                                                                       aaGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg            >  1:138072/1‑140 (MQ=255)                                                                                                                       aaGATAATTGGTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg            >  1:10649/1‑140 (MQ=255)                                                                                                                                 gTGCGAG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggggact  >  2:741657/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                        ACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTT  >  NC_000913/4605953‑4606219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑