| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 777,145 | (A)5→6 | coding (804/1266 nt) | tolA → | Tol‑Pal system protein TolA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 777,140 | 1 | . | A | 90.5% | 63.9 / 0.3 | 21 | coding (799/1266 nt) | tolA | Tol‑Pal system protein TolA |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (11/8); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
AGAAGAAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAG‑AA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGG > NC_000913/777012‑777273 | agaagaAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAgccgc > 1:268316/1‑140 (MQ=255)agaagaAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAgccgc > 1:355430/1‑140 (MQ=255) ctgctgAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACaaaaa > 1:381928/1‑140 (MQ=255) gCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCaa > 1:106758/1‑140 (MQ=255) aTAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGc > 2:345915/1‑140 (MQ=255) ggCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGaaaaa > 1:226407/1‑140 (MQ=255) gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTg < 1:1027725/140‑1 (MQ=255) aGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTgcagca < 2:312081/140‑1 (MQ=255) gACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCg > 2:614357/1‑140 (MQ=255) aCAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCACCGGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCCGAAAAAGCCGCTGCAGCAAAAGCGGCCGc < 2:106758/140‑1 (MQ=38) gCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCg < 2:425309/140‑1 (MQ=255) gCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCg < 1:865497/140‑1 (MQ=255) cGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAG‑AG‑AAAGCTGCAGCCGACAAAAAAGCAGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGt < 1:1056645/140‑1 (MQ=21) ggCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGt < 1:786370/140‑1 (MQ=255) tgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAg > 1:1064988/1‑140 (MQ=255) ctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt < 2:226407/140‑1 (MQ=255) ctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt < 1:345915/140‑1 (MQ=255) aaaaGGCAGCAGCTGATAAGAAAGCAGCGGCAG‑AATAAAGCCTCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGTAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg < 2:752825/140‑1 (MQ=255) agcagcTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGt > 2:1084369/1‑140 (MQ=255) agcagcTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGt < 1:614357/140‑1 (MQ=255) agcTGATAAGAAAGCAGCGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGGaaa > 2:168113/1‑139 (MQ=255) cGGCAGAAA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggg > 2:78030/1‑140 (MQ=255) | AGAAGAAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAG‑AA‑AAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGG > NC_000913/777012‑777273 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |