| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,875,056 | (C)6→7 | coding (521/822 nt) | nimR ← | DNA‑binding transcriptional repressor NimR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,875,050 | 1 | . | C | 97.7% | 150.7 / ‑3.4 | 44 | coding (527/822 nt) | nimR | DNA‑binding transcriptional repressor NimR |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (15/28); total (15/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGG > NC_000913/1874912‑1875184 | ccTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑c > 2:689233/1‑140 (MQ=255) cACTAATCCCTGGAGCGCCATAATCAGCTGTAACTGCTGACGCCGCTGGCGAAAACTCAACCCCGTCTCTTTTATTATCAGTCGGGCAAGATTGCGTTCACTCATCTCAAAAATGCCAGCCCACTGCCCCAATC‑ccaccc > 2:994852/1‑140 (MQ=255) cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑cccccc > 1:943346/1‑140 (MQ=255) cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑cccccc > 2:434831/1‑140 (MQ=255) cTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑ccccccca < 2:89075/140‑3 (MQ=255) cTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑ccccccca > 1:298289/1‑138 (MQ=255) ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑ccc < 1:425661/129‑1 (MQ=255) ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑ccc > 2:425661/1‑129 (MQ=255) tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAATCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAg > 2:770963/1‑140 (MQ=255) tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAg > 2:414097/1‑140 (MQ=255) gAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCTAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAgg < 1:685043/140‑1 (MQ=255) gcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCtt < 1:298283/140‑1 (MQ=255) ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCttt > 2:896888/1‑140 (MQ=255) ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑ccccccca > 2:87271/1‑123 (MQ=255) ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑ccccccca < 1:87271/125‑3 (MQ=255) aTAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCg < 1:832549/140‑1 (MQ=255) aaTCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGcc < 1:414097/140‑1 (MQ=255) cTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCttt < 1:400472/130‑1 (MQ=255) cTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCttt > 2:400472/1‑130 (MQ=255) tGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGcc < 1:131045/133‑1 (MQ=255) tGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGcc > 2:131045/1‑133 (MQ=255) tAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTcc > 1:1024996/1‑140 (MQ=255) tAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTcc > 2:438401/1‑140 (MQ=255) aaCTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTcca < 1:438401/140‑1 (MQ=255) aCTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTATTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcatc > 1:722786/1‑132 (MQ=255) aCTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTATTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcatc < 2:722786/132‑1 (MQ=255) cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcatc > 2:290193/1‑124 (MQ=255) cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcatc < 1:290193/124‑1 (MQ=255) cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc < 1:434831/140‑1 (MQ=255) cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc < 1:689233/140‑1 (MQ=255) ccACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCgg < 1:896888/140‑1 (MQ=255) cGAAACCTCAACCCCGTCTCTTTTATTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg < 1:994852/140‑1 (MQ=255) gAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg < 1:592426/140‑1 (MQ=255) aaaCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGAt < 2:298289/140‑1 (MQ=255) cTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgag < 2:1024996/140‑1 (MQ=255) aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCGTCTCCACCATCGTGCGGATTTTAGGATGagaag < 1:393739/140‑1 (MQ=255) aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGagaag < 1:68025/140‑1 (MQ=255) gTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg > 2:502802/1‑140 (MQ=255) ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc < 2:911876/140‑1 (MQ=255) ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc < 2:943346/140‑1 (MQ=255) ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc < 1:741841/140‑1 (MQ=255) ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc < 1:990439/140‑1 (MQ=255) ttttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAgg < 1:431115/140‑1 (MQ=255) tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtg > 2:889330/1‑140 (MQ=255) cAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgt > 2:217130/1‑140 (MQ=255) aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgtt > 1:758342/1‑140 (MQ=255) tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg < 2:347581/130‑1 (MQ=255) tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg > 1:347581/1‑130 (MQ=255) gATTGCGTTCACCCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgctg < 1:770963/140‑1 (MQ=255) cATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtca < 1:453545/140‑1 (MQ=255) cAAAAAAGCCAGCCCATTACCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaaa < 1:558576/140‑1 (MQ=255) agccagccCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc < 1:217130/140‑1 (MQ=255) cccAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGc < 1:502802/140‑1 (MQ=255) cAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCgg > 2:881569/1‑140 (MQ=255) | CCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGG > NC_000913/1874912‑1875184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |