Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,151,539	A→G	V149A (GTC→GCC)	exbB ←	Ton complex subunit ExbB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,151,539	0	A	G	100.0%	178.7 / NA	50	V149A (GTC→GCC)	exbB	Ton complex subunit ExbB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/20);  total (30/20)

TAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCTTTAATACCTTCG  >  NC_000913/3151405‑3151673                                                                                                                                       |                                                                                                                                       tAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTa                                                                                                                                   <  2:370986/140‑1 (MQ=255)    cGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCa                                                                                                                                >  1:998875/1‑140 (MQ=255)       ccGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAAc                                                                                                                             >  2:12185/1‑140 (MQ=255)       ccGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAAc                                                                                                                             >  1:943380/1‑140 (MQ=255)        cGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACa                                                                                                                            <  1:1084753/140‑1 (MQ=255)          cAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGa                                                                                                                          >  2:413790/1‑140 (MQ=255)          cAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGa                                                                                                                          >  1:545128/1‑140 (MQ=255)                tCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCaacg                                                                                                                    <  2:207798/140‑1 (MQ=255)                     gCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACgg                                                                                                               >  2:616267/1‑140 (MQ=255)                         cGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGaa                                                                                                           >  1:656314/1‑140 (MQ=255)                           aGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAAt                                                                                                         <  1:829721/140‑1 (MQ=255)                             gCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATcg                                                                                                       >  1:56301/1‑140 (MQ=255)                                   tGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATCCCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGa                                                                                                 <  2:422382/140‑1 (MQ=255)                                    gCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGAt                                                                                                <  2:195474/140‑1 (MQ=255)                                       gTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGt                                                                                             >  1:189948/1‑140 (MQ=255)                                                  agCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGc                                                                                  >  2:655404/1‑140 (MQ=255)                                                            aTACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACcgcg                                                                        <  2:656314/140‑1 (MQ=255)                                                                    cgcAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCAttt                                                                >  1:716521/1‑140 (MQ=255)                                                                    cgcAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCAttt                                                                >  2:558951/1‑140 (MQ=255)                                                                    cgcAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCAttt                                                                >  1:280734/1‑140 (MQ=255)                                                                      cAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTga                                                              <  1:50737/140‑1 (MQ=255)                                                                         cGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTgacga                                                           >  2:348640/1‑140 (MQ=255)                                                                             tGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCa                                                       <  1:616267/140‑1 (MQ=255)                                                                              gCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCAc                                                      <  1:558951/140‑1 (MQ=255)                                                                                  ggTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTgcg                                                  <  2:189948/140‑1 (MQ=255)                                                                                      aGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTgc                                                   >  2:285235/1‑135 (MQ=255)                                                                                      aGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTgc                                                   <  1:285235/135‑1 (MQ=255)                                                                                       gTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGAc                                             >  2:764082/1‑140 (MQ=255)                                                                                       gTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGAc                                             >  2:745160/1‑140 (MQ=255)                                                                                            cTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGAc                                        <  2:362263/140‑1 (MQ=255)                                                                                             tGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACg                                       >  2:293585/1‑140 (MQ=255)                                                                                             tGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACg                                       >  2:417247/1‑140 (MQ=255)                                                                                               cGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGcc                                     >  2:926588/1‑139 (MQ=255)                                                                                                      gcgATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACg                              <  2:280734/140‑1 (MQ=255)                                                                                                      gcgATACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACg                              <  1:417247/140‑1 (MQ=255)                                                                                                         aTACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGaa                           >  1:452630/1‑140 (MQ=255)                                                                                                          tACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAg                          <  1:764082/140‑1 (MQ=255)                                                                                                          tACCAATAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAg                          >  2:942875/1‑140 (MQ=255)                                                                                                               aTAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGt                     <  1:50943/140‑1 (MQ=255)                                                                                                               aTAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGt                     >  2:201422/1‑140 (MQ=255)                                                                                                                tAAAGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTa                    <  2:545128/140‑1 (MQ=255)                                                                                                                  aaGCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACg                  <  1:414077/140‑1 (MQ=255)                                                                                                                    gCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGtt                >  2:596585/1‑140 (MQ=255)                                                                                                                    gCTGTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGtt                >  1:869386/1‑140 (MQ=255)                                                                                                                       gTTCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCtt             >  1:476855/1‑140 (MQ=255)                                                                                                                        ttCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCttt            >  1:667360/1‑140 (MQ=255)                                                                                                                        ttCATGATGCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCttt            >  2:311556/1‑140 (MQ=255)                                                                                                                              atgCCCCAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCTTTAATAcc      <  2:667360/140‑1 (MQ=255)                                                                                                                                 ccccAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCTTTAATACCTTc   <  2:943380/140‑1 (MQ=255)                                                                                                                                  cccAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCTTTAATACCTTCg  >  1:434786/1‑140 (MQ=255)                                                                                                                                  cccAGGCCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCTTTAATACCTTCg  >  1:732538/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       TAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGGAAGCTGGTACGTTCTTTAATACCTTCG  >  NC_000913/3151405‑3151673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑