Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,230,773	T→C	V629A (GTA→GCA)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,230,773	0	T	C	100.0%	188.3 / NA	53	V629A (GTA→GCA)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

TAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCGGTGGTGAAGGTG  >  NC_000913/3230636‑3230909                                                                                                                                          |                                                                                                                                         tAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgcac                                                                                                                                        >  1:962054/1‑140 (MQ=255) tAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgcac                                                                                                                                        <  2:962054/140‑1 (MQ=255) tAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgcac                                                                                                                                        <  2:730529/140‑1 (MQ=255)   aCCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGacgcacgt                                                                                                                                      >  1:268704/1‑140 (MQ=255)     cATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTAt                                                                                                                                    <  2:417661/140‑1 (MQ=255)             ggCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATa                                                                                                                            >  1:900108/1‑140 (MQ=255)             ggCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATa                                                                                                                            >  2:470562/1‑140 (MQ=255)               cGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATaaa                                                                                                                          >  1:112407/1‑140 (MQ=255)               cGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTAGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATaaa                                                                                                                          >  2:6833/1‑140 (MQ=255)                   gaTGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCAc                                                                                                                      <  1:14371/140‑1 (MQ=255)                    aTGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACt                                                                                                                     >  1:750369/1‑140 (MQ=255)                    aTGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACt                                                                                                                     >  1:341375/1‑140 (MQ=255)                             aTCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACgg                                                                                                            <  2:750369/140‑1 (MQ=255)                              tCCTCGGTAAGCCATAAGAGTCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGc                                                                                                           >  2:417410/1‑140 (MQ=255)                              tCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGc                                                                                                           >  2:499477/1‑140 (MQ=255)                                      aaGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCggg                                                                                                   <  1:113508/140‑1 (MQ=255)                                       aGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGc                                                                                                  <  2:547051/140‑1 (MQ=255)                                         ccAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCaa                                                                                                <  1:636506/140‑1 (MQ=255)                                                   ccAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGAttgtt                                                                                      >  1:1041935/1‑140 (MQ=255)                                                   ccAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGAttgtt                                                                                      <  1:1020206/140‑1 (MQ=255)                                                   ccAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGAttgtt                                                                                      >  2:384275/1‑140 (MQ=255)                                                   ccAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTAGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGAttgtt                                                                                      <  1:6833/140‑1 (MQ=255)                                                     aaaCGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGa                                                                                    >  2:714519/1‑140 (MQ=255)                                                      aaCGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAg                                                                                   <  1:922609/140‑1 (MQ=255)                                                      aaCGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAg                                                                                   <  1:470562/140‑1 (MQ=255)                                                        cGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAgcg                                                                                 <  2:900108/140‑1 (MQ=255)                                                                             cagcTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTg                                                            >  2:707502/1‑140 (MQ=255)                                                                              agcTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAg                                                                         >  1:693809/1‑126 (MQ=255)                                                                              agcTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAg                                                                         <  2:693809/126‑1 (MQ=255)                                                                                cTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTc                                                         >  1:514624/1‑140 (MQ=255)                                                                                  cgcgGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTcgc                                                       <  2:112407/140‑1 (MQ=255)                                                                                    cgGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTcgccg                                                     <  1:499477/140‑1 (MQ=255)                                                                                     ggACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTcgccgc                                                    >  1:1052887/1‑140 (MQ=255)                                                                                            aTCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTaa                                             <  1:417410/140‑1 (MQ=255)                                                                                            aTCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTaa                                             <  2:514624/140‑1 (MQ=255)                                                                                               aaCACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACgg                                          <  2:357376/140‑1 (MQ=255)                                                                                                     tGTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGc                                    >  2:929335/1‑140 (MQ=255)                                                                                                      gTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGTGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCa                                   >  1:146043/1‑140 (MQ=255)                                                                                                      gTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCa                                   >  2:1037060/1‑140 (MQ=255)                                                                                                      gTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCa                                   >  1:19689/1‑140 (MQ=255)                                                                                                      gTATGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCa                                   >  2:66744/1‑140 (MQ=255)                                                                                                        aTGTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAAc                                 >  1:301682/1‑140 (MQ=255)                                                                                                          gTTCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGc                               <  1:912256/140‑1 (MQ=255)                                                                                                           ttCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCa                              >  1:957451/1‑140 (MQ=255)                                                                                                            tCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCATCGCAg                             >  2:68472/1‑140 (MQ=255)                                                                                                            tCGACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAg                             >  1:137462/1‑140 (MQ=255)                                                                                                              gACCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGc                           >  1:219789/1‑140 (MQ=255)                                                                                                               aCCCGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGcc                          >  2:249623/1‑140 (MQ=255)                                                                                                                 ccGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCgg                                     >  1:957928/1‑127 (MQ=255)                                                                                                                 ccGACTACCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCgg                                     <  2:957928/127‑1 (MQ=255)                                                                                                                       aCCCAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGa                  <  2:513846/140‑1 (MQ=255)                                                                                                                        cccAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCATCGCAGGCCAATGCCGAc                 <  1:68472/140‑1 (MQ=255)                                                                                                                          cAGTTCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGAcgc               <  2:268704/140‑1 (MQ=255)                                                                                                                              tCTACTATGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCggtg           <  1:707502/140‑1 (MQ=255)                                                                                                                               ctactaTGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCggtgg          >  2:344088/1‑140 (MQ=255)                                                                                                                                 actaTGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCGgtggtg        <  1:384275/140‑1 (MQ=255)                                                                                                                                  ctaTGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCGGTGGTGa       >  1:221141/1‑140 (MQ=255)                                                                                                                                  ctaTGACGCACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCGGTGGTGa       >  1:580572/1‑140 (MQ=255)                                                                                                                                       acgcacgtATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGAcgc               <  1:51018/127‑1 (MQ=255)                                                                                                                                       acgcacgtATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGAcgc               <  1:423688/127‑1 (MQ=255)                                                                                                                                       acgcacgtATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCGGTGGTGAAGGTg  >  2:963399/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGCGGACTACATCAACACCTGTATGTTCGACCCGACTACCCAGTTCTACTATGACGTACGTATTGAAGATAAACCACTGGCGAACGGCTGCGCGGGCAAACCGATTGTTGAGCGCGGTAAAGGGCCGGAAGGCTGGTCGCCGCTGTTTAACGGTGCGGCAACGCAGGCCAATGCCGACGCGGTGGTGAAGGTG  >  NC_000913/3230636‑3230909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑