Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,292,985 C→T V117V (GTG→GTA)  rsmI ← 16S rRNA 2'‑O‑ribose C1402 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,292,9850CT98.3% 197.1 / ‑5.3 58V117V (GTG→GTA) rsmI16S rRNA 2'‑O‑ribose C1402 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/29);  minor base A (1/0);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGGCTTTTAGCGCATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCTT  >  NC_000913/3292859‑3293118
                                                                                                                              |                                                                                                                                     
aaTGGCTTTTAGCGCATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATcc                                                                                                                          <  1:704136/140‑1 (MQ=255)
 aTGGCTTTTAGCGCATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTTGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATccc                                                                                                                         <  1:921073/140‑1 (MQ=255)
            cgcATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGc                                                                                                              <  1:729032/140‑1 (MQ=255)
              cATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATccc                                                                                                                         >  1:213978/1‑127 (MQ=255)
              cATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATccc                                                                                                                         <  2:213978/127‑1 (MQ=255)
              cATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAg                                                                                                            >  1:122297/1‑140 (MQ=255)
                tCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAggg                                                                                                          >  2:375931/1‑139 (MQ=255)
                tCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGt                                                                                                          >  1:627422/1‑140 (MQ=255)
                 cACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTa                                                                                                         <  2:18586/140‑1 (MQ=255)
                   cggcggCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACg                                                                                                       <  2:122297/140‑1 (MQ=255)
                   cggcggCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACg                                                                                                       <  2:676056/140‑1 (MQ=255)
                         ccTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAg                                                                                                 >  1:692117/1‑140 (MQ=255)
                         ccTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAACACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAg                                                                                                 >  1:1061016/1‑140 (MQ=255)
                          ctgttGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGa                                                                                                <  2:1005636/137‑1 (MQ=255)
                            tttGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATg                                                                                              <  1:136855/140‑1 (MQ=255)
                              tGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGt                                                                                            <  1:828755/140‑1 (MQ=255)
                              tGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGt                                                                                            >  2:985374/1‑140 (MQ=255)
                                 tttGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGc                                                                                         <  2:21319/140‑1 (MQ=255)
                                  ttGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGcc                                                                                        <  1:399306/140‑1 (MQ=255)
                                    ggCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAg                                                                                      <  2:569147/140‑1 (MQ=255)
                                    ggCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAg                                                                                      >  2:438186/1‑140 (MQ=255)
                                    ggCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAg                                                                                      <  1:985374/140‑1 (MQ=255)
                                                   ttCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAgcg                                                                       <  1:864582/140‑1 (MQ=255)
                                                            gAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCgg                                                              <  2:692117/140‑1 (MQ=255)
                                                            gAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAACACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCgg                                                              <  2:1061016/140‑1 (MQ=255)
                                                             aaaCGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGAACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGc                                                             >  1:72799/1‑140 (MQ=255)
                                                               aCGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCAt                                                           >  1:177718/1‑140 (MQ=255)
                                                                  gTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCgg                                                        >  1:682072/1‑140 (MQ=255)
                                                                  gTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGGACGCACCAGAAGGTTGCCAGGGTCGTTAATTAGCGGCGTTCCGGCATCgg                                                        >  2:1028295/1‑140 (MQ=255)
                                                                     agagGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACA‑GGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAgcgg                                                                      >  2:986272/1‑122 (MQ=255)
                                                                     agagGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACA‑GGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAgcgg                                                                      <  1:986272/122‑1 (MQ=255)
                                                                      gagGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACAACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAAc                                                    >  2:565463/1‑140 (MQ=255)
                                                                             aaCCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCa                                                            >  1:385077/1‑125 (MQ=255)
                                                                             aaCCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCa                                                            <  2:385077/125‑1 (MQ=255)
                                                                             aaCCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCa                                             <  1:1028295/140‑1 (MQ=255)
                                                                                   cTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGttt                                       >  1:592546/1‑140 (MQ=255)
                                                                                   cTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGttt                                       >  1:1062317/1‑140 (MQ=255)
                                                                                   cTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGttt                                       >  2:967595/1‑140 (MQ=255)
                                                                                   cTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGttt                                       >  2:910324/1‑140 (MQ=255)
                                                                                   cTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGttt                                       >  1:778760/1‑140 (MQ=255)
                                                                                   cTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGttt                                       >  1:291750/1‑140 (MQ=255)
                                                                                      cgcTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGc                                    <  1:375931/140‑1 (MQ=255)
                                                                                       gcTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGcc                                   <  1:967595/140‑1 (MQ=255)
                                                                                       gcTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGcc                                   <  2:778760/140‑1 (MQ=255)
                                                                                                aGTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCa                          <  2:1062317/140‑1 (MQ=255)
                                                                                                 gTGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAg                         >  2:29628/1‑140 (MQ=255)
                                                                                                  tGATAGCAGCACACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGc                        >  1:791877/1‑140 (MQ=255)
                                                                                                          gcaCACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCcag                >  1:584521/1‑140 (MQ=255)
                                                                                                          gcaCACGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCcag                >  2:804637/1‑140 (MQ=255)
                                                                                                           cacaCGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCcagc               >  1:67022/1‑140 (MQ=255)
                                                                                                           cacaCGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCcagc               <  2:584521/140‑1 (MQ=255)
                                                                                                            acacGGCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCcagca              >  1:493432/1‑140 (MQ=255)
                                                                                                                ggCCCGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGt          >  1:845644/1‑140 (MQ=255)
                                                                                                                    cGGGTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCa      <  1:438186/140‑1 (MQ=255)
                                                                                                                       gTAGCGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCt   <  2:67022/140‑1 (MQ=255)
                                                                                                                        tAGCGGTACCACGCGGATCCCAGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCtt  <  2:250329/140‑1 (MQ=255)
                                                                                                                           cGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGttt        >  1:193852/1‑131 (MQ=255)
                                                                                                                           cGGTACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGttt        <  2:193852/131‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
AATGGCTTTTAGCGCATCACGGCGGCCTTTTGATTTGGCAGGTAAAAAGCCTTCGTAACAGAAACGGTCAGAGGGTAAACCCGCTGCGCTTAACGCAGTGATAGCAGCACACGGCCCGGGTAGCGGCACCACGCGGATCCCCGCTTCACGGCAGGTACGCACCAGATGGTAGCCAGGATCGTTAATTAGCGGCGTTCCGGCATCGGAAACCAGCGCAATGTTTTGCCCCTCTTGCAGCTTCGCCAGCAGCGTTTCAGCTT  >  NC_000913/3292859‑3293118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑