Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	301,619	A→G	V90A (GTA→GCA)	paoB ←	aldehyde dehydrogenase, FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	301,619	0	A	G	100.0%	205.1 / NA	58	V90A (GTA→GCA)	paoB	aldehyde dehydrogenase, FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/28);  total (30/28)

CCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGCCCGAGGCCGTTCACATCGAT  >  NC_000913/301483‑301743                                                                                                                                         |                                                                                                                             cccGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCcg                                                                                                                           >  1:870416/1‑140 (MQ=255)      gCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTaa                                                                                                                      >  2:417997/1‑140 (MQ=255)      gCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTaa                                                                                                                      >  2:398244/1‑140 (MQ=255)      gCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTaa                                                                                                                      >  1:516990/1‑140 (MQ=255)         ggCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCa                                                                                                                   <  1:417997/140‑1 (MQ=255)         ggCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCa                                                                                                                   <  1:398244/140‑1 (MQ=255)          gcgcTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCac                                                                                                                  >  2:553278/1‑140 (MQ=255)          gcgcTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTCCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCac                                                                                                                  >  1:359136/1‑140 (MQ=255)                aTTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGacgca                                                                                                            <  2:516990/140‑1 (MQ=255)                      gggCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCt                                                                                                      <  1:288464/140‑1 (MQ=255)                            aTTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCacg                                                                                                                 >  1:1011420/1‑123 (MQ=255)                            aTTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAg                                                                                                >  1:294007/1‑140 (MQ=255)                            aTTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAg                                                                                                >  2:197471/1‑140 (MQ=255)                            aTTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAg                                                                                                >  2:1034749/1‑140 (MQ=255)                            aTTGGTGTCGTAAAAATAGGGGCAGAGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCacg                                                                                                                 <  2:1011420/123‑1 (MQ=255)                                 tgtCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAgccgcc                                                                                           >  2:13012/1‑140 (MQ=255)                                   tCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAg                                                                                         >  1:240716/1‑140 (MQ=255)                                     gTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGt                                                                                       <  2:240716/140‑1 (MQ=255)                                     gTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGt                                                                                       <  2:870416/140‑1 (MQ=255)                                     gTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGt                                                                                       <  1:13012/140‑1 (MQ=255)                                            tAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGtt                                                                                <  2:294007/140‑1 (MQ=255)                                               gggCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCg                                                                                     <  1:1031889/132‑1 (MQ=255)                                               gggCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCg                                                                                     >  2:1031889/1‑132 (MQ=255)                                                   aGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTAcc                                                                         >  2:441506/1‑140 (MQ=255)                                                    gcgcGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCa                                                                        >  1:51026/1‑140 (MQ=255)                                                         tGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTgcg                                                                   >  1:1065305/1‑140 (MQ=255)                                                          gcgcTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTgcgc                                                                  >  2:413907/1‑140 (MQ=255)                                                                    aGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAg                                                        <  2:511645/140‑1 (MQ=255)                                                                      aTTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGcc                                                      >  2:455479/1‑140 (MQ=255)                                                                      aTTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGcc                                                      >  2:601487/1‑140 (MQ=255)                                                                      aTTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGcc                                                      >  2:128959/1‑140 (MQ=255)                                                                          ccTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCAcc                                                  >  1:134598/1‑140 (MQ=255)                                                                              cGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCcgcg                                              <  1:1034749/140‑1 (MQ=255)                                                                                gtggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTc                                            >  2:590845/1‑140 (MQ=255)                                                                                  ggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCgg                                          <  1:441506/140‑1 (MQ=255)                                                                                  ggtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCgg                                          <  2:51026/140‑1 (MQ=255)                                                                                   gtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt                                         <  1:553278/140‑1 (MQ=255)                                                                                   gtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt                                         <  1:590845/140‑1 (MQ=255)                                                                                   gtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt                                         <  1:128959/140‑1 (MQ=255)                                                                                   gtTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt                                         <  1:455479/140‑1 (MQ=255)                                                                                    ttGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCACGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTc                                        >  1:516356/1‑140 (MQ=255)                                                                                      gCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCAc                                      <  2:359136/140‑1 (MQ=255)                                                                                         tGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt                                         <  2:914302/134‑1 (MQ=255)                                                                                         tGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt                                         >  1:914302/1‑134 (MQ=255)                                                                                           aTTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCaa                                 >  2:371885/1‑140 (MQ=255)                                                                                            ttACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAAt                                <  1:256576/140‑1 (MQ=255)                                                                                                  aaCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATc                          <  1:413907/140‑1 (MQ=255)                                                                                                  aaCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATc                          <  1:197471/140‑1 (MQ=255)                                                                                                     tGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAg                       >  1:211223/1‑140 (MQ=255)                                                                                                      gACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGc                      >  1:958538/1‑140 (MQ=255)                                                                                                       aCCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGcc                     >  2:418910/1‑140 (MQ=255)                                                                                                       aCCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGcc                     <  2:567221/140‑1 (MQ=255)                                                                                                         cAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATc                          <  2:994452/133‑1 (MQ=255)                                                                                                         cAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATc                          >  1:994452/1‑133 (MQ=255)                                                                                                         cAGACGCGCCAGCGAGCAGGGCGCGGGAGAGGGCCGTGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGCCGGTGTTCCGCACCAGTGCGCCGATCCGCAGACCACCCGCGCCGGTCACTTCAATCTTATCGAGCCCg                   >  2:833907/1‑140 (MQ=255)                                                                                                           gACGCGCCAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGCCCGAg                 <  2:958538/140‑1 (MQ=255)                                                                                                                 ccAGCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGCCCGAGGCCGtt           <  1:584950/140‑1 (MQ=255)                                                                                                                    gCGAGCAGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGCCCGAGGCCGTTcac        <  2:134598/140‑1 (MQ=255)                                                                                                                          aGGGCGCGGGAGAGTGCCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGCCCGAGGCCGTTCACATCGAt  >  1:627410/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             CCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTTCAATCTTATCGAGCCCGAGGCCGTTCACATCGAT  >  NC_000913/301483‑301743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑